FR2313394A1 - Enzymes immobilised on polymers contg. aldehyde and acid gps. - with pH - deppedent solubility - Google Patents

Enzymes immobilised on polymers contg. aldehyde and acid gps. - with pH - deppedent solubility

Info

Publication number
FR2313394A1
FR2313394A1 FR7517762A FR7517762A FR2313394A1 FR 2313394 A1 FR2313394 A1 FR 2313394A1 FR 7517762 A FR7517762 A FR 7517762A FR 7517762 A FR7517762 A FR 7517762A FR 2313394 A1 FR2313394 A1 FR 2313394A1
Authority
FR
France
Prior art keywords
gps
aldehyde
enzyme
acid
solubility
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7517762A
Other languages
French (fr)
Other versions
FR2313394B1 (en
Inventor
Ekkehard Van Leemputten
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe des Produits Nestle SA
Nestle SA
Original Assignee
Societe des Produits Nestle SA
Nestle SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Societe des Produits Nestle SA, Nestle SA filed Critical Societe des Produits Nestle SA
Priority to FR7517762A priority Critical patent/FR2313394A1/en
Publication of FR2313394A1 publication Critical patent/FR2313394A1/en
Application granted granted Critical
Publication of FR2313394B1 publication Critical patent/FR2313394B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers

Abstract

Products with enzymatic activity are produced by reacting an enzyme with a polymer contg. both free aldehyde gps and free acid gps. The products have variable solubility i.e. they can be used to carry out enzyme-catalysed reaction-in a homogeneous liq. medium but are readily sepd from the medium after reaction by adjusting the pH. The polymer is pref. a copolymer of acrolein and acrylic acid contg 10% free aldehyde gps and >=5% free acid gps. the latter based on the total content of free aldehyde and acid gps. The enzyme is pref. trypsin. The products are pref. prepd by mixing the enzyme and polymer in a buffer soln. (esp. pH8), precipitating the product and filtering it off.
FR7517762A 1975-06-06 1975-06-06 Enzymes immobilised on polymers contg. aldehyde and acid gps. - with pH - deppedent solubility Granted FR2313394A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7517762A FR2313394A1 (en) 1975-06-06 1975-06-06 Enzymes immobilised on polymers contg. aldehyde and acid gps. - with pH - deppedent solubility

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7517762A FR2313394A1 (en) 1975-06-06 1975-06-06 Enzymes immobilised on polymers contg. aldehyde and acid gps. - with pH - deppedent solubility

Publications (2)

Publication Number Publication Date
FR2313394A1 true FR2313394A1 (en) 1976-12-31
FR2313394B1 FR2313394B1 (en) 1979-05-04

Family

ID=9156155

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7517762A Granted FR2313394A1 (en) 1975-06-06 1975-06-06 Enzymes immobilised on polymers contg. aldehyde and acid gps. - with pH - deppedent solubility

Country Status (1)

Country Link
FR (1) FR2313394A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2905154A1 (en) * 1978-02-13 1979-08-30 Technicon Instr PARTICULATE REAGENTS FOR IMMUNOASSAYS
DE2900736A1 (en) * 1979-01-10 1980-08-14 Ir I Orch Chimii Sib Otdel Aka Hydroxy-sulphonic acid deriv. of acrolein!-acrylic! acid copolymer - useful as blood coagulating inhibitor to prevent thrombogenic and cardiac diseases
EP0087786A1 (en) * 1982-02-28 1983-09-07 Yeda Research And Development Company, Ltd. Agarose-polyaldehyde beads, process for their production and their use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NEANT *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2905154A1 (en) * 1978-02-13 1979-08-30 Technicon Instr PARTICULATE REAGENTS FOR IMMUNOASSAYS
DE2900736A1 (en) * 1979-01-10 1980-08-14 Ir I Orch Chimii Sib Otdel Aka Hydroxy-sulphonic acid deriv. of acrolein!-acrylic! acid copolymer - useful as blood coagulating inhibitor to prevent thrombogenic and cardiac diseases
EP0087786A1 (en) * 1982-02-28 1983-09-07 Yeda Research And Development Company, Ltd. Agarose-polyaldehyde beads, process for their production and their use

Also Published As

Publication number Publication date
FR2313394B1 (en) 1979-05-04

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