FI88309B - Ny biotest - Google Patents
Ny biotest Download PDFInfo
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- FI88309B FI88309B FI891899A FI891899A FI88309B FI 88309 B FI88309 B FI 88309B FI 891899 A FI891899 A FI 891899A FI 891899 A FI891899 A FI 891899A FI 88309 B FI88309 B FI 88309B
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- cell
- plasmid
- recombinant dna
- protein
- dna
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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Claims (21)
1. Förfarande för bestämning av en faktor, som inverkar pä en encellig organism, säsom tili exempel en mikrob- eller jästcell, vilken faktor inverkar direkt eller indirekt pä 5 cellens DNA, RNA och/eller proteiner eller deras syntesme-kanismer, kännetecknat av att a) intill cellen införs en rekombinant-DNA-plasmid, vars initiationsställe eller -ställen av dupliceringsmekanism 10 ansvarig för replikationen är avhängiga av en regulerbar promotor, som kan kontrolleras antingen med en positiv eller en negativ feedback; b) cellen innehällande rekombinant-DNA-plasmiden bringas i 15 kontakt med en effektiv faktor, tili exempel genom att inku- bera dem tillsammans; c) den effektiva faktorn tkr inverka pä cellen innehdllande rekombinant-DNA-plasmiden för en lämplig tid, varefter pro- 20 motorn, som reglerar initiationsstället av dupliceringsme- kanismen ansvarig för rekombinant-DNA-plasmidens replikation aktiveras, varvid plasmidens kopital börjar öka i cellen, om den effektiva faktorn inte har inverkat pä ett hindrande sätt pä plasmidens replikation; 25 d) ändring av rekombinant-DNA-plasmidens kopital i cellen bestäms direkt eller indirekt; e) kopitalet jämförs med ett kontrollprov, väri ingen effek-30 tiv faktor var närvarande och/eller den fanns i en känd mängd i reaktionen, varvid närvaron och/eller mängden av den effektiva faktorn kan bestämmas.
2. Förfarande enligt patentkravet l, kännetecknat av att <’> rekombinant-DNA-plasmiden innehäller en DNA-sekvens, som ko- dar en eller flera selektiva proteiner eller en för biolo-gisk aktivitet väsentlig del därav av en virus, prokariot-cell eller eukariotcell och plasmiden kan innehälla en eller 4i 8 8 309 flera DNA-sekvenser, som gör cellen resistent mot anti-biotika, tungmetaller och/eller toxiner.
3. Förfarande enligt patentkravet 2, k&nnetecknat av att 5 DNA-sekvensen som kodar proteinet är avhängig av en reguler- bar promotor, som kontrolleras antingen med en positiv eller en negativ feedback och är regulerbar i använda mikrobcellen samt aktiveras samtidigt eller pä önskad tidpunkt efter ak-tiveringen av promotorn som reglerar initiationsstället av 10 replikationen.
4. Förfarande enligt patentkravet 3, k&nnetecknat av att faktoren som skall bestämmas är en faktor som inverkar pä rekombinant-DNA-plasmidens DNA biosyntes eller replikation,
5. Förfarande enligt patentkravet 4, k&nnetecknat av att cellen är Escherichia coli och dupliceringsmekanismen ansva- 25 rig för rekombinant-DNA-plasmidens replikation i bakterie kan strängt regleras med en kraftig promotor, säsom lambda λ. PL- och lac-promotorer.
6. Förfarande för bestämning av en faktor som inverkar pä 30 en cell, vilken faktor inverkar direkt eller indirekt pä cellens DNA, RNA och/eller proteiner eller deras syntesme-kanismer,'k&nnetecknat av att a) intill cellen införs en rekombinant-DNA-plasmid, som 35 finns i cellen pä flera kopior och plasmiden innehäller som markerprotein en DNA-sekvens, som kodar ett protein eller en för biologisk aktivitet väsentlig del därav av en virus, prokariotcell eller eukariotcell, vilken DNA-sekvens är av- 42 8 8 3 ϋ 9 hängiga av en regulerbar promotor och är kontrollerad antin- gen med en positiv eller en negativ feedback; b) cellen innehällande rekombinant-DNA-plasmiden bringas i 5 kontakt med en effektiv faktor, tili exempel genom att inku-bera dem tillsammans; c) den effektiva faktorn fär inverka pä cellen innehällande rekombinant-DNA-plasmiden för en lämplig tid, varefter den 10 regulerbara promotorn, som kontrollerar expression av rekom-binant-DNA-plasmidens markerprotein aktiveras, varvid mar-kerproteinets mängd börjar oka i cellen, om den effektiva faktorn inte har inverkat direkt eller indirekt pä protein-syntesen; 15 d) ändring av markerproteinet i rekombinant-DNA-plasmiden mäts; e) markerproteinets mängd jämförs med ett kontrollprov, väri 20 ingen effektiv faktor var närvarande och/eller den fanns i en känd mängd i reaktionen, varvid närvaron och/eller mäng-den av den effektiva faktorn kan bestämmas.
7. Förfarande enligt patentkrav l eller 6, kannetecknat av 25 att cellen är en gramnegativ eller grampositiv bakterie som hör tili gruppen Enterobacteriaceae, företrädesvis Escherichia coli eller tili gruppen Bacillus, företrädesvis Bacillus subtilis. 30
8. Förfarande enligt patentkraven 1-3, 6 eller 7, kanne tecknat av att promotorns reguleringsfaktorer produceras utav värdcellens kromosomala DNA, utav en andra tili en annan inkombatibilitetsklass hörande plasmid i cellen, utav densamma plasmiden, utav en lytisk eller lysogenisk fag el-35 ler virus eller reguleringsfaktorer tillsätts utifrän celler säsom tili exempel genom tillsättning av kemiska föreningar, förändring av temperaturen eller genom strälning. 43 8 8 3 C 9
9. Förfarande enligt patentkravet 8, kannetecknat av att rekombinant-DNA-plasmiden kan innehälla en eller flera DNA-sekvenser, som gör cellen resistent mot antibiotika, tungme-taller och/eller toxiner. 5
10. Förfarande enligt patentkravet 1 eller 6, kannetecknat av att provet som skall undersökas är i en flytande eller fast form eller provet innehäller radioaktiv strälning eller ultravioletsträlning. 10
11. Förfarande enligt patentkravet 1 eller 6, k&nnetecknat av att cellerna är lyofiliserade och de rehydreras före bes-tämningen med en lämplig buffert eller odlingslösning. 15
12. Förfarande enligt patentkravet 6, kännetecknat av att den effektiva faktorn är en faktor som inverkar pä rekom-binant-DNA-plasmidens DNA-biosyntes, RNA-biosyntes, transkription, translation, cellmembraner eller metabolism, säsom mutagener, antibiotika, tungmetaller eller toxiner. 20
13. Förfarande enligt patentkravet 12, k&nnetecknat av att cellen är Bacillus subtilis.
14. Förfarande enligt patentkravet 13, k&nnetecknat av att 25 markerproteinet i rekombinant-DNA-plasmiden är avhängig av en regulerbar stark promotor, säsom ol05, och markerproteinet är en alfa-amylas, alkalisk fosfatas eller luciferas.
15. Förfarande enligt patentkravet 12, k&nnetecknat av att 30 cellen är Escherichia coli.
15 RNA-biosyntes, transkription, translation, cellmembraner, metabolism eller enzyxnaktivitet, säsom tili exempel aflato-xiner, tungmetaller, etidiumbromid, nalidixinsyra, trimeto-prim, fluorokinoloner, aminoglykosider, penicilliner, kefa-losporiner, rifampicin, kloramfenikol, tetracykliner, sul-20 fonamider och den använda cellen är sensitiv mot de bestämda antibiotika.
16. Förfarande enligt patentkravet 5 eller 15, k&nnetecknat av att rekombinant-DNA-plasmiden innehäller en DNA-sek-vens, som kodar ett protein eller en för biologisk aktivi- '35 tet väsentlig del därav av en virus, prokariotcell eller eukariotcell, och proteinets expression kan kontrolleras med en regulerbar promotor, säsom tili exempel lac eller lambda 44 8 8 3 G 9
17. Förfarande enligt patentkravet 16, kännetecknat av att proteinet är luciferas, beta-galaktosidas, alkalisk fosfa-tas, peroksidas eller T4-lysozym. 5
18. Förfarande enligt patentkravet 17, kännetecknat av att rekombinant-DNA-plasmiden kodar luciferas av Vibrio harvey eller en annan bakterieluciferas.
19. Förfarande enligt patentkravet 18, kännetecknat av att 10 rekombinant-DNA-plasmiden är plasmiden pCSS112.
20. Förfarande enligt nägot av patentkraven 17-19, känne-tecknad av att aldehyd tillsätts tili reaktionen när mängden av det uttryckta luciferasenzymet i bakterien mäts. 15
21. Förfarande enligt patentkravet 4 eller 12, kännetecknat av att bestämningen görs utav mjölk, serum eller vatten.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI891899A FI88309C (sv) | 1989-04-20 | 1989-04-20 | Ny biotest |
| PCT/FI1990/000112 WO1990012887A1 (en) | 1989-04-20 | 1990-04-20 | Determination of factors affecting gene regulation and/or gene replication |
| DE69023642T DE69023642T2 (de) | 1989-04-20 | 1990-04-20 | Bestimmung der die genregelung und/oder genreplikation betreffenden faktoren. |
| US07/768,741 US6475719B1 (en) | 1989-04-20 | 1990-04-20 | Determination of factors affecting gene regulation and/or gene replication |
| EP90906232A EP0469021B1 (en) | 1989-04-20 | 1990-04-20 | Determination of factors affecting gene regulation and/or gene replication |
| ES90906232T ES2081981T3 (es) | 1989-04-20 | 1990-04-20 | Determinacion de los factores que afectan la regulacion y/o replicacion de genes. |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI891899A FI88309C (sv) | 1989-04-20 | 1989-04-20 | Ny biotest |
| FI891899 | 1989-04-20 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI891899A0 FI891899A0 (fi) | 1989-04-20 |
| FI891899A FI891899A (fi) | 1990-10-21 |
| FI88309B true FI88309B (fi) | 1993-01-15 |
| FI88309C FI88309C (sv) | 1993-04-26 |
Family
ID=8528282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI891899A FI88309C (sv) | 1989-04-20 | 1989-04-20 | Ny biotest |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6475719B1 (sv) |
| EP (1) | EP0469021B1 (sv) |
| DE (1) | DE69023642T2 (sv) |
| ES (1) | ES2081981T3 (sv) |
| FI (1) | FI88309C (sv) |
| WO (1) | WO1990012887A1 (sv) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE280219T1 (de) * | 1992-03-16 | 2004-11-15 | Jacob Nathaniel Wohlstadter | Mutagenese und selektionsmethoden in den selben wirtszellen |
| FI95484C (sv) * | 1994-01-17 | 1996-02-12 | Marko Virta | Förvarande för bestämning av metall i provet |
| WO1996001899A1 (en) * | 1994-07-08 | 1996-01-25 | Schering Corporation | METHOD FOR IDENTIFYING NUCLEIC ACIDS ENCODING c-fos PROMOTER ACTIVATING PROTEINS |
| US5504093A (en) * | 1994-08-01 | 1996-04-02 | Otsuka Pharmaceutical Co., Ltd. | Method for inhibiting nucleoside and nucleobase transport in mammalian cells, and method for inhibition of DNA virus replication |
| US5736331A (en) * | 1995-07-08 | 1998-04-07 | Schering Corporation | Method for identifying nucleic acids encoding c-fos promoter activating proteins |
| US7132247B1 (en) | 1998-09-17 | 2006-11-07 | Regents Of The University Of Minnesota | Composite devices incorporating biological material and methods |
| WO2000037676A1 (en) * | 1998-12-22 | 2000-06-29 | Isis Innovation Limited | Method of sequence identification |
| GB9910499D0 (en) * | 1999-05-06 | 1999-07-07 | Azur Env Ltd | Assay reagent |
| DE602004029868D1 (de) * | 2003-03-05 | 2010-12-16 | Chemo Sero Therapeut Res Inst | Verfahren zur herstellung eines heterologen proteins in e. coli |
| EP2363025A1 (en) | 2003-03-27 | 2011-09-07 | PTC Therapeutics, Inc. | Targeting enzymes of the TRNA splicing pathway for identification of anti-fungal and/or anti-proliferative molecules |
| JP4634371B2 (ja) | 2003-03-27 | 2011-02-16 | ピーティーシー セラピューティクス，インコーポレーテッド | ｔＲＮＡスプライシングエンドヌクレアーゼに標的化する化合物の同定方法、および該化合物の抗増殖剤としての使用 |
| WO2005014805A1 (en) | 2003-08-08 | 2005-02-17 | Regents Of The University Of Minnesota | A structured material for the production of hydrogen |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE424090B (sv) | 1981-08-18 | 1982-06-28 | Technion Res & Dev Foundation | Forfarande for bestemning av en antibiotikakoncentration |
| US4806471A (en) * | 1982-09-16 | 1989-02-21 | A/S Alfred Benzon | Plasmids with conditional uncontrolled replication behavior |
| GB8308236D0 (en) | 1983-03-25 | 1983-05-05 | Celltech Ltd | Dna vectors |
| DE3882394T2 (de) * | 1987-03-03 | 1993-12-16 | Light Oil Utilization Res Ass | Plasmide. |
-
1989
- 1989-04-20 FI FI891899A patent/FI88309C/sv active IP Right Grant
-
1990
- 1990-04-20 EP EP90906232A patent/EP0469021B1/en not_active Expired - Lifetime
- 1990-04-20 US US07/768,741 patent/US6475719B1/en not_active Expired - Fee Related
- 1990-04-20 DE DE69023642T patent/DE69023642T2/de not_active Expired - Fee Related
- 1990-04-20 WO PCT/FI1990/000112 patent/WO1990012887A1/en active IP Right Grant
- 1990-04-20 ES ES90906232T patent/ES2081981T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69023642D1 (de) | 1995-12-21 |
| EP0469021A1 (en) | 1992-02-05 |
| US6475719B1 (en) | 2002-11-05 |
| FI891899A0 (fi) | 1989-04-20 |
| DE69023642T2 (de) | 1996-10-17 |
| WO1990012887A1 (en) | 1990-11-01 |
| FI891899A (fi) | 1990-10-21 |
| ES2081981T3 (es) | 1996-03-16 |
| FI88309C (sv) | 1993-04-26 |
| EP0469021B1 (en) | 1995-11-15 |
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