FI73317B - KYVETTENHET. - Google Patents

Description

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The present invention relates to a cuvette for reading the results of virological, bacteriological and hematological reactions or for spectrophotometric measurement of chemical color reactions, in which light is passed to the

10 Such quartet systems are already known e.g.

in accordance with Finnish patent 57665 (corresponding to British patent 1,436,210). However, they have often had the disadvantage of diffuse light entering the detector if the area of the cuvette bottom is considerably larger than the area of the light beam passing through it.

U.S. Pat. No. 3,773,426 discloses a test plate for monitoring bacterial growth, in which the bottom of the cup-shaped sample containers has a very small transparent window of different material. The device therefore has to be made of two 20 different materials, which already makes manufacturing cumbersome.

A cuvette as defined in the claims has now been invented.

In the present invention, the bottom of the cuvette is in two parts, with a highly light-transmitting area polished in the middle of the bottom and a dull matte annular area at the edges of the bottom, of which only a small part of the scattered light is scattered.

The area is preferably annular, but other shapes are also possible, e.g. one which forms a square of the measuring window.

An additional advantage of the cuvette according to the invention is that the so-called In EIA technology, the so-called sensitization procedure can be used to adhere more biological molecules to the matte area of the bottom of the cuvette than to the glossy surface. This is due to the fact that the contact surface of the liquid with the plastic in my matte nnns is larger than k i i11 intake-1a a 1ueel1 a.

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The invention will become more apparent from the following description and the accompanying drawings, in which Figure 1 shows a cuvette in side view, Figure 2 shows a cuvette in plan view, Figure 3 shows a single cuvette in three-dimensional view and section, Figure 1 shows a single cuvette with light sources and detectors in section .

The support plate 2 of the cuvette 1 has several vertical cuvettes 3 at certain intervals 3. These act as cuvette paths for measuring the absorbance of the solution in the cuvette 3 or the change in light intensity caused by the precipitate or turbidity at the bottom of the cuvette 3. The light passes from the light guide 14 through the lens 13 15 through the bottom 5 of the cuvette 3 and the light intensity is registered through the lens 15 by a detector 6 above the cuvette.

The measuring window 5 of the cuvettes 3 of the cuvette 1 is surrounded on the outside by a cylindrical part or edge 20 7, which protects the measuring window from dirt and scratches, whereby the measuring windows 5 of the cuvettes of the cuvette remain optically high. If the bottom 5 of the cuvettes 3 of the cuvette 1 is planar, the bottom of the cuvette is easy to manufacture by conventional means and does not have an optical error of 25% £. nor the effect of the lens on the passage of light.

The measuring windows 5 of the cuvettes 3 are provided with an annular, matt surface area 3 and 9 at their edges, either on one or both sides of the window.

The beam of the measuring light passing through the bottom window 5 of the cuvette is most preferably clearly smaller in diameter than the annular matt surface area 3, whereby the matt surface 3 does not affect the passage of the measuring light. Instead, the stray light 11 possibly coming from the environment or reflected from the bottom is scattered from the matte surface 3 in all directions *, whereby only a fraction of the stray light 11 is exposed to the detector 35.

The bottom of the cuvette has an annular matte intestinal part 3 en

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3 7331 7 larger than a smooth surface. This can be utilized in the so-called. In EIA technology, where the so-called the sensitization procedure is used to attach biological molecules, such as antigens or antibodies, to the plastic walls of the cuvette. As the contact between the liquid and the plastic increases in the nat-5 pin region, more molecules adhere to the surface of the plastic during sensitization than if it had been polished smooth. The roughness of the matt surface can thus be used to control the properties of the cuvettes sensitized for EIA technology. Most preferably, the roughness, i.e. the E-value, can be adjusted by the so-called by spark machining to suit and desired.

Claims (4)

7331 7 Cuvette system made of a single light-transmitting material, in which the measuring windows are at the bottom of the cuvettes, characterized in that the measuring window is surrounded by a matt light-scattering area (8). Cuvette system according to Claim 1, characterized in that the matt surface area is annular (8). Cuvette system according to Claim 1 or 2, characterized in that the matt surface area (8) is inside the cuvette. Cuvette system according to Claim 1 or 2, characterized in that there is a matt surface area (8) inside and outside the measuring window.