FI3760705T3 - Novel microalgal strain of thraustochytrium genus and method of producing polyunsaturated fatty acid using the same - Google Patents

Novel microalgal strain of thraustochytrium genus and method of producing polyunsaturated fatty acid using the same Download PDF

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Publication number
FI3760705T3
FI3760705T3 FIEP19825193.6T FI19825193T FI3760705T3 FI 3760705 T3 FI3760705 T3 FI 3760705T3 FI 19825193 T FI19825193 T FI 19825193T FI 3760705 T3 FI3760705 T3 FI 3760705T3
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Finland
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microalgae
docosahexaenoic acid
cjm01
genus thraustochytrium
product
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FIEP19825193.6T
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Finnish (fi)
Swedish (sv)
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Ji Young Kim
Myung Geun Park
Hye Min Park
Jung Woon Choi
Sang Min Park
Sang Young Bae
Jin Sook Chang
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C12N1/125—Unicellular algae isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (9)

PATENTTIVAATIMUKSETPATENT CLAIMS 1. Thraustochytrium-suvun CJM01-mikrolevä, jonka tallennusnumero on KCTC 13538BP, jolla dokosahek- saeenihappotuotto (DHA) kohoaa ja aminohappotuotto ale- nee verrattuna emokantaan.1. CJM01 microalgae of the genus Thraustochytrium, whose registration number is KCTC 13538BP, with which docosahexaenoic acid (DHA) production increases and amino acid production decreases compared to the parent strain. 2. Patenttivaatimuksen 1 mukainen mikrolevä, jossa Thraustochytrium-suvun CJM01-mikrolevä sisältää dokosaheksaeenihappoa määränä, joka on 40 - 60 paino- prosenttia rasvahappojen kokonaispainoon perustuen.2. The microalgae according to claim 1, wherein the CJM01 microalgae of the genus Thraustochytrium contains docosahexaenoic acid in an amount that is 40 to 60 percent by weight based on the total weight of fatty acids. 3. Patenttivaatimuksen 1 mukainen mikrolevä, jossa Thraustochytrium-suvun CJM01-mikrolevällä dokosa- heksaeenihapon tuottavuus on 0,5 - 0,7 g/1/h.3. The microalgae according to claim 1, where the productivity of docosahexaenoic acid is 0.5 to 0.7 g/1/h with the CJM01 microalgae of the genus Thraustochytrium. 4. Menetelmä biomassan tuottamiseksi, menetel- män käsittäessä: patenttivaatimuksen 1 mukaisen Thraustochyt- rium-suvun CJM01-mikrolevän viljelemisen; ja mikrolevän dokosaheksaeenihappoa sisältävän biomassan, sen vilje- lytuotteen, sen kuivatun tuotteen tai sen jauhetun tuot- teen talteenottamisen mikrolevästä.4. A method for producing biomass, the method comprising: cultivating the CJM01 microalgae of the genus Thraustochytrium according to claim 1; and recovering the docosahexaenoic acid-containing biomass of the microalgae, its culture product, its dried product or its ground product from the microalgae. 5. Patenttivaatimuksen 4 mukainen menetelmä, jossa viljeleminen suoritetaan heterotrofisissa olosuh- teissa.5. The method according to claim 4, in which the cultivation is carried out under heterotrophic conditions. 6. Patenttivaatimuksen 4 mukainen menetelmä, jossa viljeleminen suoritetaan käyttämällä ravintoalus- taa, joka sisältää hiilenlähdettä ja typenlähdettä.6. The method according to claim 4, in which cultivation is carried out using a nutrient medium that contains a carbon source and a nitrogen source. 7. Patenttivaatimuksen 6 mukainen menetelmä, jossa hiilenlähde on ainakin yksi, joka valitaan ryh- mästä, joka koostuu glukoosista, fruktoosista, maltoo- sista, galaktoosista, mannoosista, sakkaroosista, ara- binoosista, ksyloosista ja glyserolista.7. The method according to claim 6, wherein the carbon source is at least one selected from the group consisting of glucose, fructose, maltose, galactose, mannose, sucrose, arabinose, xylose and glycerol. 8. Patenttivaatimuksen 6 mukainen menetelmä, jossa typenlähde on i) orgaaninen typenlähde, joka va- litaan ryhmästä, joka koostuu hiivauutteesta, 1i- hauutteesta, peptonista ja tryptonista, tai ii) epäor- gaaninen typenlähde, joka valitaan ryhmästä, joka koos- tuu ammoniumasetaatista, ammoniumnitraatista,8. The method according to claim 6, in which the nitrogen source is i) an organic nitrogen source selected from the group consisting of yeast extract, 1i extract, peptone and tryptone, or ii) an inorganic nitrogen source selected from the group consisting of ammonium acetate , of ammonium nitrate, ammoniumkloridista, ammoniumsulfaatista, natriumnitraa- tista, ureasta ja mononatriumglutamaatista (MSC).of ammonium chloride, ammonium sulfate, sodium nitrate, urea and monosodium glutamate (MSC). 9. Menetelmä bioöljyn tuottamiseksi, menetel- män käsittäessä: patenttivaatimuksen 1 mukaisen Thraus- tochytrium-suvun CJM01-mikrolevän viljelemisen; ja mik- rolevän dokosaheksaeenihappoa (DHA) sisältävän lipidin, sen viljelytuotteen, sen kuivatun tuotteen tai sen jau- hetun tuotteen talteenottamisen mikrolevästä.9. A method for producing bio-oil, the method comprising: cultivating the CJM01 microalgae of the genus Thraustochytrium according to claim 1; and recovering the docosahexaenoic acid (DHA)-containing lipid of the microalgae, its culture product, its dried product or its powdered product from the microalgae.
FIEP19825193.6T 2018-06-29 2019-06-26 Novel microalgal strain of thraustochytrium genus and method of producing polyunsaturated fatty acid using the same FI3760705T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020180075945A KR102100650B1 (en) 2018-06-29 2018-06-29 Novel microalgal strain of Thraustochytrium genus, and producing polyunsaturated fatty acids using the same
PCT/KR2019/007697 WO2020004924A1 (en) 2018-06-29 2019-06-26 Novel strain of thraustochytrium genus and polyunsaturated fatty acid production method using same

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CN (1) CN112513247B (en)
AR (1) AR115667A1 (en)
AU (1) AU2019292277B2 (en)
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CA (1) CA3095279C (en)
CL (1) CL2020002416A1 (en)
DK (1) DK3760705T3 (en)
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HU (1) HUE060352T2 (en)
MY (1) MY194114A (en)
RU (1) RU2754686C1 (en)
TW (1) TWI715088B (en)
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KR20230066973A (en) 2021-11-08 2023-05-16 씨제이제일제당 (주) Novel strain of Schizochytrium sp. with easy intracellular oil extraction and a method for producing oil containing omega3 using the same
KR20230066972A (en) 2021-11-08 2023-05-16 씨제이제일제당 (주) Novel strain of Schizochytrium sp. with easy intracellular oil extraction and a method for producing oil containing omega3 using the same

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WO2020004924A1 (en) 2020-01-02
AU2019292277A1 (en) 2021-02-04
RU2754686C1 (en) 2021-09-06
BR112020019053A2 (en) 2021-01-12
AR115667A1 (en) 2021-02-10
CL2020002416A1 (en) 2020-12-28
CA3095279C (en) 2023-04-04
UY38286A (en) 2019-08-30
US11773417B2 (en) 2023-10-03
HUE060352T2 (en) 2023-02-28
DK3760705T3 (en) 2023-01-09
US20210002680A1 (en) 2021-01-07
EP3760705B1 (en) 2022-11-09
AU2019292277B2 (en) 2022-09-01
JP2021514645A (en) 2021-06-17
CN112513247A (en) 2021-03-16
JP6947941B2 (en) 2021-10-13
CN112513247B (en) 2023-11-14
TWI715088B (en) 2021-01-01
BR112020019053B1 (en) 2021-12-07
EP3760705A1 (en) 2021-01-06
CA3095279A1 (en) 2020-01-02
TW202016289A (en) 2020-05-01
EP3760705A4 (en) 2021-05-19
MY194114A (en) 2022-11-14
BR112020019053B8 (en) 2022-01-25
ES2932536T3 (en) 2023-01-20
KR20200002448A (en) 2020-01-08

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