FI20002114A - Rekombinanttientsyymien käyttö GDP-L-fukoosin ja fukosyloitujen glykaanien valmistamiseksi - Google Patents

Rekombinanttientsyymien käyttö GDP-L-fukoosin ja fukosyloitujen glykaanien valmistamiseksi Download PDF

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Publication number
FI20002114A
FI20002114A FI20002114A FI20002114A FI20002114A FI 20002114 A FI20002114 A FI 20002114A FI 20002114 A FI20002114 A FI 20002114A FI 20002114 A FI20002114 A FI 20002114A FI 20002114 A FI20002114 A FI 20002114A
Authority
FI
Finland
Prior art keywords
gdp
fucose
preparation
recombinant enzymes
fucosylated glycans
Prior art date
Application number
FI20002114A
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English (en)
Swedish (sv)
Other versions
FI109811B (fi
FI20002114A0 (fi
Inventor
Risto Renkonen
Pirkko Mattila
Laura Hirves
Solveing Hortling
Tuula Kallioinen
Sirkka-Liisa Kauranen
Nina Jaervinen
Minna Maeki
Jaana Niittymaeki
Jarkko Raebinae
Original Assignee
Medicel Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicel Oy filed Critical Medicel Oy
Priority to FI20002114A priority Critical patent/FI109811B/fi
Publication of FI20002114A0 publication Critical patent/FI20002114A0/fi
Priority to EP01660180A priority patent/EP1199364A3/en
Priority to US09/962,805 priority patent/US20020058313A1/en
Publication of FI20002114A publication Critical patent/FI20002114A/fi
Application granted granted Critical
Publication of FI109811B publication Critical patent/FI109811B/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0703—Fucose-1-phosphate guanylyltransferase (2.7.7.30)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FI20002114A 2000-09-26 2000-09-26 Menetelmä GDP-L-fukoosin valmistamiseksi ja menetelmässä käytettäviä välineitä FI109811B (fi)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FI20002114A FI109811B (fi) 2000-09-26 2000-09-26 Menetelmä GDP-L-fukoosin valmistamiseksi ja menetelmässä käytettäviä välineitä
EP01660180A EP1199364A3 (en) 2000-09-26 2001-09-25 Use of recombinant enzymes for preparing GDP-L-fucose and fucosylated glycans
US09/962,805 US20020058313A1 (en) 2000-09-26 2001-09-26 Use of recombinant enzymes for preparing GDP-L-fucose and fucosylated glycans

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20002114 2000-09-26
FI20002114A FI109811B (fi) 2000-09-26 2000-09-26 Menetelmä GDP-L-fukoosin valmistamiseksi ja menetelmässä käytettäviä välineitä

Publications (3)

Publication Number Publication Date
FI20002114A0 FI20002114A0 (fi) 2000-09-26
FI20002114A true FI20002114A (fi) 2002-03-27
FI109811B FI109811B (fi) 2002-10-15

Family

ID=8559160

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20002114A FI109811B (fi) 2000-09-26 2000-09-26 Menetelmä GDP-L-fukoosin valmistamiseksi ja menetelmässä käytettäviä välineitä

Country Status (3)

Country Link
US (1) US20020058313A1 (fi)
EP (1) EP1199364A3 (fi)
FI (1) FI109811B (fi)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020132320A1 (en) * 2001-01-10 2002-09-19 Wang Peng George Glycoconjugate synthesis using a pathway-engineered organism
ES2381104T3 (es) * 2001-10-29 2012-05-23 Crucell Holland B.V. Métodos y medios para producir proteínas con modificaciones postraduccionales predeterminadas
JP5236189B2 (ja) 2003-12-05 2013-07-17 チルドレンズ ホスピタル メディカル センター オリゴ糖組成物および感染症の治療における該組成物の使用
US7326770B2 (en) * 2004-01-22 2008-02-05 Neose Technologies, Inc. H. pylori fucosyltransferases
FI20055398A0 (fi) 2005-07-08 2005-07-08 Suomen Punainen Risti Veripalv Menetelmä solupopulaatioiden evaluoimiseksi
CA2698374C (en) 2007-09-07 2018-04-03 Children's Hospital Medical Center Use of secretor, lewis and sialyl antigen levels as predictors for disease
EP2166085A1 (en) 2008-07-16 2010-03-24 Suomen Punainen Risti Veripalvelu Divalent modified cells
CA2767043C (en) 2009-07-06 2020-07-14 Children's Hospital Medical Center Inhibiting inflammation with milk oligosaccharides
EP2895592A1 (en) 2011-03-23 2015-07-22 Société Industrielle Limousine d'Application Biologique (SILAB) A yeast recombinant cell capable of producing gdp-fucose
KR101152876B1 (ko) 2011-04-15 2012-06-12 서울대학교산학협력단 구아노신-이노신 키나아제의 추가 발현에 의한 재조합 대장균에서 ｇｄｐ-푸코즈의 생산방법
KR101152878B1 (ko) 2011-04-15 2012-06-12 서울대학교산학협력단 글루코오스-6-포스페이트-데하이드로지나아제 및 구아노신-이노신 키나아제의 추가 발현에 의한 재조합 대장균에서 ｇｄｐ-푸코즈의 생산방법
EP2852610B1 (en) 2012-05-23 2018-07-11 Glykos Finland Oy Production of fucosylated glycoproteins
WO2014130789A1 (en) 2013-02-21 2014-08-28 Children's Hospital Medical Center Use of glycans and glycosyltransferases for diagnosing/monitoring inflammatory bowel disease
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
WO2016176484A1 (en) 2015-04-28 2016-11-03 Children's Hospital Medical Center Use of oligosaccharide compositions to enhance weight gain
EP3719135A1 (en) * 2019-04-01 2020-10-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Enzymatic method for preparation of gdp-fucose

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100436593C (zh) * 1996-09-17 2008-11-26 协和发酵工业株式会社 复合糖类的制备方法
US7244601B2 (en) * 1997-12-15 2007-07-17 National Research Council Of Canada Fusion proteins for use in enzymatic synthesis of oligosaccharides
AU5559399A (en) * 1998-08-14 2000-03-06 Research Foundation Of The State University Of New York, The Vanadate resistance glycosylation 4 gene

Also Published As

Publication number Publication date
US20020058313A1 (en) 2002-05-16
FI109811B (fi) 2002-10-15
FI20002114A0 (fi) 2000-09-26
EP1199364A2 (en) 2002-04-24
EP1199364A3 (en) 2004-03-24

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