FI109811B - Förfarande för framställning av GDP-L-fukos och vid förfarandet använda medel - Google Patents

Förfarande för framställning av GDP-L-fukos och vid förfarandet använda medel Download PDF

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FI109811B
FI109811B FI20002114A FI20002114A FI109811B FI 109811 B FI109811 B FI 109811B FI 20002114 A FI20002114 A FI 20002114A FI 20002114 A FI20002114 A FI 20002114A FI 109811 B FI109811 B FI 109811B
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Finland
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gdp
fucose
gmd
mannose
gfs
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FI20002114A
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English (en)
Finnish (fi)
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FI20002114A0 (sv
FI20002114A (sv
Inventor
Risto Renkonen
Pirkko Mattila
Laura Hirvas
Solveig Hortling
Tuula Kallioinen
Sirkka-Liisa Kauranen
Nina Jaervinen
Minna Maeki
Jaana Niittymaeki
Jarkko Raebinae
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Medicel Oy
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Priority to FI20002114A priority Critical patent/FI109811B/sv
Publication of FI20002114A0 publication Critical patent/FI20002114A0/sv
Priority to EP01660180A priority patent/EP1199364A3/en
Priority to US09/962,805 priority patent/US20020058313A1/en
Publication of FI20002114A publication Critical patent/FI20002114A/sv
Application granted granted Critical
Publication of FI109811B publication Critical patent/FI109811B/sv

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0703—Fucose-1-phosphate guanylyltransferase (2.7.7.30)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (15)

1. Förfarande för framställning av GDP-L-fukos av GDP-D-mannos, kännetecknat avatt man framställer GDP-L-fukos frän inneboende
5 GDP-D-mannos genom att odla celler av jäst eller mögel, vilka har transforme-rats med en DNA-sekvens som kodar för GDP-mannos-4,6-dehydratas (GMD) och en DNA-sekvens som kodar för GDP~4-keto-6-deoxi-D-mannos-3,5-epimeras/4-reduktas (GFS) för att funktionellt uttrycka nämnda enzym, och tar tillvara GDP-L-fukosen som bildas av GDP-D-mannosen som förekommer na-10 turligt i nämnda celler.
2. Förfarande enligt patentkrav 1, kännetecknat av att det GMD och GFS som uttrycks är enzym av Escherichia coli eller Helicobacter pylori.
3. Förfarande enligt patentkrav 1, kännetecknat av att man 15 transformerar jästceller med en vektor som innehäller nämnda DNA-sekvenser inkorporerade i en enda vektor.
4. Förfarande enligt patentkrav 3, kännetecknat av att man transformerar cellerna med en vektor som kodar en chimärisk molekyl av GMD ;:· och GFS. : ’ ·!* 20 5. Förfarande enligt nägot av patentkraven 1-4, känneteck- ·"*· n a t av att jästcellerna är Saccharomyces cerevisiae. :· 6. Förfarande enligt patentkrav 1, kännetecknat av att man :: ytterligare framställer fukosylerade glykaner av den GDP-L-fukos som bildas. «»*
7. Förfarande enligt patentkrav 6, kännetecknat av att de 25 fukosylerade glykanerna framställs av GDP-L-fukos medelst rekombinant fu- : 1: kosyltransferas.
8. Förfarande enligt patentkrav 7, kännetecknat av att fuko- ; ' · · syltransferaset är a-1,3-fukosyltransferas.
9. Förfarande enligt patentkrav 8, kännetecknat av att a- : ’: 30 1,3-fukosyltransferaset är a-1,3-fukosyltransferas av rätta eller bakterie. : 10. Förfarande enligt patentkrav 9, kännetecknat av att a- 1,3-fukosyltransferaset är Helicobacter felis a-1,3-fukosyltransferas. 34 10981 1
11. Förfarande enligt patentkrav 1, kännetecknat avatt cel-lerna transformeras med en DNA-sekvens som kodar Helicobacter pylori GDP-mannos-4,6-dehydratas (GMD).
12. Förfarande enligt patentkrav 1 eller 11, kännetecknat av 5 att cellerna transformeras med en DNA-sekvens som kodar Helicobacter pylori GDP-4-keto-6-deoxi-D-mannos-3,5-epimeras/4-reduktas (GFS).
13. Chimäriskt enzym, kännetecknat av att det är en chimä-risk molekyl av GDP-mannos-4,6-dehydratas (GMD) och GDP-4-keto-6-deoxi-D-mannos-3,5-epimeras/4-reduktas (GFS).
14. Vektor, kännetecknad av att den innehäller en DNA- sekvens som kodar en chimerisk molekyl av GDP-mannos-4,6-dehydratas (GMD) och GDP-4-keto-6-deoxi-D-mannos-3,5-epimeras/4-reduktas (GFS).
15. Jäst- eller mögelcell, kännetecknad av att den innehäller en vektor enligt patentkrav 14. 15 « • · a • · « · · • · · « * • · • i i t · • · • I · • · ti» f * · » « * » · • · · t · I t * • · · • · tl» • i > · ·
FI20002114A 2000-09-26 2000-09-26 Förfarande för framställning av GDP-L-fukos och vid förfarandet använda medel FI109811B (sv)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FI20002114A FI109811B (sv) 2000-09-26 2000-09-26 Förfarande för framställning av GDP-L-fukos och vid förfarandet använda medel
EP01660180A EP1199364A3 (en) 2000-09-26 2001-09-25 Use of recombinant enzymes for preparing GDP-L-fucose and fucosylated glycans
US09/962,805 US20020058313A1 (en) 2000-09-26 2001-09-26 Use of recombinant enzymes for preparing GDP-L-fucose and fucosylated glycans

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20002114A FI109811B (sv) 2000-09-26 2000-09-26 Förfarande för framställning av GDP-L-fukos och vid förfarandet använda medel
FI20002114 2000-09-26

Publications (3)

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FI20002114A0 FI20002114A0 (sv) 2000-09-26
FI20002114A FI20002114A (sv) 2002-03-27
FI109811B true FI109811B (sv) 2002-10-15

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US (1) US20020058313A1 (sv)
EP (1) EP1199364A3 (sv)
FI (1) FI109811B (sv)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020132320A1 (en) * 2001-01-10 2002-09-19 Wang Peng George Glycoconjugate synthesis using a pathway-engineered organism
CA2465007C (en) * 2001-10-29 2012-01-17 Crucell Holland B.V. Methods and means for producing proteins with predetermined post-translational modifications
US7893041B2 (en) * 2003-12-05 2011-02-22 Children's Hospital Medical Center Oligosaccharide compositions and use thereof in the treatment of infection
US7326770B2 (en) * 2004-01-22 2008-02-05 Neose Technologies, Inc. H. pylori fucosyltransferases
FI20055398A0 (sv) 2005-07-08 2005-07-08 Suomen Punainen Risti Veripalv Förfarande för att evaluera cellpopulationer
CA2698374C (en) 2007-09-07 2018-04-03 Children's Hospital Medical Center Use of secretor, lewis and sialyl antigen levels as predictors for disease
EP2166085A1 (en) 2008-07-16 2010-03-24 Suomen Punainen Risti Veripalvelu Divalent modified cells
CA2767043C (en) 2009-07-06 2020-07-14 Children's Hospital Medical Center Inhibiting inflammation with milk oligosaccharides
EP2895592A1 (en) 2011-03-23 2015-07-22 Société Industrielle Limousine d'Application Biologique (SILAB) A yeast recombinant cell capable of producing gdp-fucose
KR101152878B1 (ko) 2011-04-15 2012-06-12 서울대학교산학협력단 글루코오스-6-포스페이트-데하이드로지나아제 및 구아노신-이노신 키나아제의 추가 발현에 의한 재조합 대장균에서 ｇｄｐ-푸코즈의 생산방법
KR101152876B1 (ko) 2011-04-15 2012-06-12 서울대학교산학협력단 구아노신-이노신 키나아제의 추가 발현에 의한 재조합 대장균에서 ｇｄｐ-푸코즈의 생산방법
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
WO2014130789A1 (en) 2013-02-21 2014-08-28 Children's Hospital Medical Center Use of glycans and glycosyltransferases for diagnosing/monitoring inflammatory bowel disease
US10513724B2 (en) 2014-07-21 2019-12-24 Glykos Finland Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
US10857167B2 (en) 2015-04-28 2020-12-08 Children's Hospital Medical Center Use of oligosaccharide compositions to enhance weight gain
EP3719135A1 (en) * 2019-04-01 2020-10-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Enzymatic method for preparation of gdp-fucose

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Publication number Priority date Publication date Assignee Title
CN100342025C (zh) * 1996-09-17 2007-10-10 协和发酵工业株式会社 糖核苷酸类及复合糖类的制备方法
US7244601B2 (en) * 1997-12-15 2007-07-17 National Research Council Of Canada Fusion proteins for use in enzymatic synthesis of oligosaccharides
WO2000009550A1 (en) * 1998-08-14 2000-02-24 The Research Foundation Of State University Of New York Vanadate resistance glycosylation 4 gene

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FI20002114A0 (sv) 2000-09-26
EP1199364A2 (en) 2002-04-24
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EP1199364A3 (en) 2004-03-24
US20020058313A1 (en) 2002-05-16

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