ES8606654A1 - Un procedimiento para la preparacion de un glucoronido. - Google Patents

Un procedimiento para la preparacion de un glucoronido.

Info

Publication number
ES8606654A1
ES8606654A1 ES529118A ES529118A ES8606654A1 ES 8606654 A1 ES8606654 A1 ES 8606654A1 ES 529118 A ES529118 A ES 529118A ES 529118 A ES529118 A ES 529118A ES 8606654 A1 ES8606654 A1 ES 8606654A1
Authority
ES
Spain
Prior art keywords
steroid hormone
alkaline phosphatase
glucoronides
phosphatase labeled
labeled steroid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES529118A
Other languages
English (en)
Other versions
ES529118A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of ES529118A0 publication Critical patent/ES529118A0/es
Publication of ES8606654A1 publication Critical patent/ES8606654A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PREPARACION DE HORMONAS ESTEROIDES-GLUCORONIDOS MARCADAS CON ENZIMAS. CONSISTENTE EN ESTERIFICAR EL GRUPO CARBONILO LIBRE DEL GLUCONIDO DE UNA HORMONA ESTEROIDICA CON N-HIDROXISUCCIMIDA EN PRESENCIA DE HIDROCLORURO DE 1-ETIL-3-(3-DIMETILAMINOPROPIL)CARBODIIMIDA EN DIMETIL FORMAMIDA, Y HACER REACCIONAR EL ESTER ACTIVO OBTENIDO CON FOSFATASA ALCALINA EN PROPORCION 200:1 Y 100:1 FORMANDOSE ASI LA CORRESPONDIENTE UNION AMIDO. LA HORMONA ESTEROIDICA PUEDE SER ESTRADIOL, ESTRIOL, PREGNANODIOL O ESTRONA. UTILES COMO TRAZADORES EN INMUNOENSAYOS ENZIMATICOS.
ES529118A 1983-01-25 1984-01-24 Un procedimiento para la preparacion de un glucoronido. Expired ES8606654A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US46073483A 1983-01-25 1983-01-25

Publications (2)

Publication Number Publication Date
ES529118A0 ES529118A0 (es) 1986-04-01
ES8606654A1 true ES8606654A1 (es) 1986-04-01

Family

ID=23829873

Family Applications (1)

Application Number Title Priority Date Filing Date
ES529118A Expired ES8606654A1 (es) 1983-01-25 1984-01-24 Un procedimiento para la preparacion de un glucoronido.

Country Status (3)

Country Link
EP (1) EP0114615A2 (es)
JP (1) JPS59138957A (es)
ES (1) ES8606654A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0389164A (ja) * 1989-08-31 1991-04-15 Meidensha Corp 酵素免疫測定用試薬及びその調製方法
CN100360938C (zh) * 2003-04-23 2008-01-09 北京科美东雅生物技术有限公司 甾体类激素-碱性磷酸酶结合物制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54135759A (en) * 1978-04-14 1979-10-22 Kureha Chem Ind Co Ltd Amino sugar-steroid hormone combination

Also Published As

Publication number Publication date
EP0114615A2 (en) 1984-08-01
ES529118A0 (es) 1986-04-01
JPS59138957A (ja) 1984-08-09

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970612