Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Antibioticos SA
Original Assignee
Antibioticos SA
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Filing date
Publication date
Application filed by Antibioticos SAfiledCriticalAntibioticos SA
Priority to ES545213ApriorityCriticalpatent/ES8604974A1/en
Publication of ES8604974A1publicationCriticalpatent/ES8604974A1/en
Publication of ES545213A0publicationCriticalpatent/ES545213A0/en
Priority to DE8686305447Tprioritypatent/DE3685160D1/en
Priority to EP86305447Aprioritypatent/EP0209363B1/en
Priority to AT86305447Tprioritypatent/ATE75751T1/en
The process comprises (a) incubating free extracts of cells of Penicillium chysogenum AS-P-78 with Isopenicillin N-synthesase activity, in presence of phenylacetyl L-cysteinyl D-valine in a reaction medium of Tris-HCl buffer 30 mM, ditrotreitol (DTT) 6 mm, FeSO4 0.1 mM and sodium ascorbate 1 mm at pH 8-10 and 20-37 deg.C for 2 hrs. to cyclise the synthetic hetero-peptide and obtain Penicillin G.