Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SAfiledCriticalAntibioticos SA
Priority to ES545213ApriorityCriticalpatent/ES8604974A1/en
Publication of ES545213A0publicationCriticalpatent/ES545213A0/en
Publication of ES8604974A1publicationCriticalpatent/ES8604974A1/en
Priority to EP86305447Aprioritypatent/EP0209363B1/en
Priority to DE8686305447Tprioritypatent/DE3685160D1/en
Priority to AT86305447Tprioritypatent/ATE75751T1/en
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines
(AREA)
Abstract
The process comprises (a) incubating free extracts of cells of Penicillium chysogenum AS-P-78 with Isopenicillin N-synthesase activity, in presence of phenylacetyl L-cysteinyl D-valine in a reaction medium of Tris-HCl buffer 30 mM, ditrotreitol (DTT) 6 mm, FeSO4 0.1 mM and sodium ascorbate 1 mm at pH 8-10 and 20-37 deg.C for 2 hrs. to cyclise the synthetic hetero-peptide and obtain Penicillin G.
Streptomyces mutant strains lacking branched-chain 2-oxo acid dehydrogenase activity and avermectin b o-methyl-transferase activity and their use for production of b avermectins