ES8603568A1 - Procedimiento para obtener un cultivo celular - Google Patents

Procedimiento para obtener un cultivo celular

Info

Publication number
ES8603568A1
ES8603568A1 ES542143A ES542143A ES8603568A1 ES 8603568 A1 ES8603568 A1 ES 8603568A1 ES 542143 A ES542143 A ES 542143A ES 542143 A ES542143 A ES 542143A ES 8603568 A1 ES8603568 A1 ES 8603568A1
Authority
ES
Spain
Prior art keywords
cell cultures
obtaining cell
kidneys
obtaining
contg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES542143A
Other languages
English (en)
Other versions
ES542143A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of ES8603568A1 publication Critical patent/ES8603568A1/es
Publication of ES542143A0 publication Critical patent/ES542143A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Chemical And Physical Treatments For Wood And The Like (AREA)

Abstract

PROCEDIMIENTO PARA OBTENER UN CULTIVO CELULAR. CONSISTE EN: A) DISGREGAR RIÑONES DE CERDOS SACRIFICADOS Y FORMAR UNA SUSPENSION ACUOSA; B) AÑADIR AL RESULTADO DE A) UNA SOLUCION DE COLAGENASA HASTA ALCANZAR UNA CONCENTRACION DE 100-150 MG POR LITRO DE COLAGENASA, Y AGITAR DURANTE 12 A 24 HORAS A UNA TEMPERATURA ENTRE 16 Y 22JC; C) SEPARAR POR CENTRIFUGACION A 800 D G, Y LAVADO CON PBS LAS CELULAS INDIVIDUALES OBTENIDAS EN B), Y D) RESUSPENDER EL SEDIMENTO CELULAR CENTRIFUGADO EN MEDIOS DE CULTIVO COMO MEM DE EAGLE O EN TCM 199 EN RELACION 1:250 A 1:350, SE ADICIONA SUERO DE TERNERO EN PROPORCION 100 ML/L Y SE DISTRIBUYE EN RECIPIENTES DE CULTIVO QUE SE MANTIENEN ESTACIONARIOS A 37JC. SE UTILIZAN PARA LA OBTENCION DE SUSPENSIONES DE VIRUS COMO ANTIGENOS PARA VACUNAS.
ES542143A 1984-04-13 1985-04-11 Procedimiento para obtener un cultivo celular Granted ES542143A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843413960 DE3413960A1 (de) 1984-04-13 1984-04-13 Verfahren zur gewinnung von zellkulturen

Publications (2)

Publication Number Publication Date
ES8603568A1 true ES8603568A1 (es) 1985-12-16
ES542143A0 ES542143A0 (es) 1985-12-16

Family

ID=6233485

Family Applications (1)

Application Number Title Priority Date Filing Date
ES542143A Granted ES542143A0 (es) 1984-04-13 1985-04-11 Procedimiento para obtener un cultivo celular

Country Status (7)

Country Link
EP (1) EP0158285B1 (es)
AT (1) ATE61815T1 (es)
DE (2) DE3413960A1 (es)
DK (1) DK171576B1 (es)
ES (1) ES542143A0 (es)
IE (1) IE57767B1 (es)
PT (1) PT80265B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009022916B4 (de) * 2009-05-27 2011-05-19 Dst Dauermagnet-System Technik Gmbh Magnetkupplung sowie Spalttopf für eine Magnetkupplung

Also Published As

Publication number Publication date
ATE61815T1 (de) 1991-04-15
PT80265A (de) 1985-05-01
DK167685D0 (da) 1985-04-12
EP0158285A2 (de) 1985-10-16
IE57767B1 (en) 1993-04-07
IE850932L (en) 1985-10-13
DK171576B1 (da) 1997-01-20
DK167685A (da) 1985-10-14
DE3582186D1 (de) 1991-04-25
EP0158285B1 (de) 1991-03-20
EP0158285A3 (en) 1987-10-21
ES542143A0 (es) 1985-12-16
PT80265B (pt) 1987-09-30
DE3413960A1 (de) 1985-10-17

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