ES8602834A1 - Nuevo procedimiento de extraccion y purificacion de ganglio-sidos - Google Patents
Nuevo procedimiento de extraccion y purificacion de ganglio-sidosInfo
- Publication number
- ES8602834A1 ES8602834A1 ES540861A ES540861A ES8602834A1 ES 8602834 A1 ES8602834 A1 ES 8602834A1 ES 540861 A ES540861 A ES 540861A ES 540861 A ES540861 A ES 540861A ES 8602834 A1 ES8602834 A1 ES 8602834A1
- Authority
- ES
- Spain
- Prior art keywords
- alkyl
- types
- methanol
- concns
- chloroform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Abstract
PROCEDIMIENTO DE EXTRACCION Y PURIFICACION GANGLIOSIDOS. CONSISTE EN: A) HOMOGENEIZAR UNA CANTIDAD DE CEREBRO BOBINO, OVINO O PORCINO EN METANOL/AGUA (70:30) CON AGENTES TENSIOACTIVOS ANIONICOS, CATIONICOS Y ANFOTEROS A CONCENTRACIONES POR DEBAJO Y POR ENCIMA DE SUS CORRESPONDIENTES CONCENTRACIONES MICELARES CRITICAS INORGANICAS; B) PURIFICAR EL EXTRACTO OBTENIDO EN HPLC/TLC EN COLUMNA DE GEL DE SILICE Y CON ELUCION EN GRADIENTE UTILIZANDO COMO SOLVENTE A: CLOROFORMO/METANOL/H2O 0,05 TFA (60:35:8) Y COMO SOLVENTE B: CLOROFORMO/METANOL/H2O 00,5 TFA (65:25:4) Y C) DESALINIZAR EL EXTRACTO DE GANGLIOSIDO LIBRE DE CONTAMINANTE EN COLUMNA C-18 SEMIPREPARATIVA O EN CARTUCHO SEP-PACK C-18 ELUYENDO CON H20 Y CLOROFORMO/METANOL (2:1); LOS AGENTES TENSIOACTIVOS PUEDEN SER: ANIONICOS TIPO ALQUIL SULFATO O ALQUIL ETER SULFATO; CATIONICOS TIPO HALUROS DE N-ALQUIL TRIMETIL AMONIO O HALUROS DE DIMETIL ALQUIL BENCIL AMONIO; NO IONICOS TIPO ALQUIL FENOL O CONDENSADOS DE OXIDO DE ETILENO; ANFOTEROS DEL TIPO ALQUIL AMIDO BETAINAS. SE UTILIZAN POR PARTICIPACION EN ENFERMEDADES DEL SISTEMA NERVIOSO Y TUMORES CANCEROSOS.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES540861A ES8602834A1 (es) | 1985-03-01 | 1985-03-01 | Nuevo procedimiento de extraccion y purificacion de ganglio-sidos |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES540861A ES8602834A1 (es) | 1985-03-01 | 1985-03-01 | Nuevo procedimiento de extraccion y purificacion de ganglio-sidos |
Publications (2)
Publication Number | Publication Date |
---|---|
ES540861A0 ES540861A0 (es) | 1985-11-16 |
ES8602834A1 true ES8602834A1 (es) | 1985-11-16 |
Family
ID=8488779
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES540861A Expired ES8602834A1 (es) | 1985-03-01 | 1985-03-01 | Nuevo procedimiento de extraccion y purificacion de ganglio-sidos |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES8602834A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2750308T3 (es) | 2015-05-22 | 2020-03-25 | Bioiberica | Proceso para preparar un extracto de cerebro de un animal |
-
1985
- 1985-03-01 ES ES540861A patent/ES8602834A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES540861A0 (es) | 1985-11-16 |
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