ES8602834A1 - Nuevo procedimiento de extraccion y purificacion de ganglio-sidos - Google Patents

Nuevo procedimiento de extraccion y purificacion de ganglio-sidos

Info

Publication number
ES8602834A1
ES8602834A1 ES540861A ES540861A ES8602834A1 ES 8602834 A1 ES8602834 A1 ES 8602834A1 ES 540861 A ES540861 A ES 540861A ES 540861 A ES540861 A ES 540861A ES 8602834 A1 ES8602834 A1 ES 8602834A1
Authority
ES
Spain
Prior art keywords
alkyl
types
methanol
concns
chloroform
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES540861A
Other languages
English (en)
Other versions
ES540861A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES540861A priority Critical patent/ES8602834A1/es
Publication of ES540861A0 publication Critical patent/ES540861A0/es
Publication of ES8602834A1 publication Critical patent/ES8602834A1/es
Expired legal-status Critical Current

Links

Landscapes

  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCEDIMIENTO DE EXTRACCION Y PURIFICACION GANGLIOSIDOS. CONSISTE EN: A) HOMOGENEIZAR UNA CANTIDAD DE CEREBRO BOBINO, OVINO O PORCINO EN METANOL/AGUA (70:30) CON AGENTES TENSIOACTIVOS ANIONICOS, CATIONICOS Y ANFOTEROS A CONCENTRACIONES POR DEBAJO Y POR ENCIMA DE SUS CORRESPONDIENTES CONCENTRACIONES MICELARES CRITICAS INORGANICAS; B) PURIFICAR EL EXTRACTO OBTENIDO EN HPLC/TLC EN COLUMNA DE GEL DE SILICE Y CON ELUCION EN GRADIENTE UTILIZANDO COMO SOLVENTE A: CLOROFORMO/METANOL/H2O 0,05 TFA (60:35:8) Y COMO SOLVENTE B: CLOROFORMO/METANOL/H2O 00,5 TFA (65:25:4) Y C) DESALINIZAR EL EXTRACTO DE GANGLIOSIDO LIBRE DE CONTAMINANTE EN COLUMNA C-18 SEMIPREPARATIVA O EN CARTUCHO SEP-PACK C-18 ELUYENDO CON H20 Y CLOROFORMO/METANOL (2:1); LOS AGENTES TENSIOACTIVOS PUEDEN SER: ANIONICOS TIPO ALQUIL SULFATO O ALQUIL ETER SULFATO; CATIONICOS TIPO HALUROS DE N-ALQUIL TRIMETIL AMONIO O HALUROS DE DIMETIL ALQUIL BENCIL AMONIO; NO IONICOS TIPO ALQUIL FENOL O CONDENSADOS DE OXIDO DE ETILENO; ANFOTEROS DEL TIPO ALQUIL AMIDO BETAINAS. SE UTILIZAN POR PARTICIPACION EN ENFERMEDADES DEL SISTEMA NERVIOSO Y TUMORES CANCEROSOS.
ES540861A 1985-03-01 1985-03-01 Nuevo procedimiento de extraccion y purificacion de ganglio-sidos Expired ES8602834A1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES540861A ES8602834A1 (es) 1985-03-01 1985-03-01 Nuevo procedimiento de extraccion y purificacion de ganglio-sidos

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES540861A ES8602834A1 (es) 1985-03-01 1985-03-01 Nuevo procedimiento de extraccion y purificacion de ganglio-sidos

Publications (2)

Publication Number Publication Date
ES540861A0 ES540861A0 (es) 1985-11-16
ES8602834A1 true ES8602834A1 (es) 1985-11-16

Family

ID=8488779

Family Applications (1)

Application Number Title Priority Date Filing Date
ES540861A Expired ES8602834A1 (es) 1985-03-01 1985-03-01 Nuevo procedimiento de extraccion y purificacion de ganglio-sidos

Country Status (1)

Country Link
ES (1) ES8602834A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2750308T3 (es) 2015-05-22 2020-03-25 Bioiberica Proceso para preparar un extracto de cerebro de un animal

Also Published As

Publication number Publication date
ES540861A0 (es) 1985-11-16

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