ES8403521A1 - "procedimiento para la obtencion del plasmido pac 1". - Google Patents

"procedimiento para la obtencion del plasmido pac 1".

Info

Publication number
ES8403521A1
ES8403521A1 ES516639A ES516639A ES8403521A1 ES 8403521 A1 ES8403521 A1 ES 8403521A1 ES 516639 A ES516639 A ES 516639A ES 516639 A ES516639 A ES 516639A ES 8403521 A1 ES8403521 A1 ES 8403521A1
Authority
ES
Spain
Prior art keywords
production
plasmid pac
pac
plasmid
kilobases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES516639A
Other languages
English (en)
Other versions
ES516639A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES8403521A1 publication Critical patent/ES8403521A1/es
Publication of ES516639A0 publication Critical patent/ES516639A0/es
Granted legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

CONSISTE EN LA PURIFICACION MEDIANTE CENTRIFUGACION EN CLORURO DE CESIO, DEL ADN TOTAL OBTENIDO DE LISADOS DE PROTOPLASTOS O MICELIOS DISGREGADOS MECANICAMENTE DE ACREMONIUM CHRYSOGENUM ATCC 14533; SEPARACION CROMATOGRAFICA DEL ADN CIRCULAR; AISLAMIENTO DEL ADN DE PLASMIDO Y PURIFICACION DE ESTE POR CENTRIFUGACIONES SUCESIVAS EN CLORURO DE CESIO.ESTE COMPUESTO TIENE APLICACIONES PARA LA OBTENCION DE UN VECTOR HIBRIDO ACTIVADOR DE LA BIOSINTESIS DE ANTIBIOTICOS DEL TIPO DE B-LACTAMA.
ES516639A 1981-10-21 1982-10-19 "procedimiento para la obtencion del plasmido pac 1". Granted ES516639A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19813141691 DE3141691A1 (de) 1981-10-21 1981-10-21 Plasmid pac 1, verfahren zu seiner gewinnung und seine verwendung

Publications (2)

Publication Number Publication Date
ES8403521A1 true ES8403521A1 (es) 1983-07-16
ES516639A0 ES516639A0 (es) 1983-07-16

Family

ID=6144526

Family Applications (1)

Application Number Title Priority Date Filing Date
ES516639A Granted ES516639A0 (es) 1981-10-21 1982-10-19 "procedimiento para la obtencion del plasmido pac 1".

Country Status (10)

Country Link
US (1) US4506014A (es)
EP (1) EP0077557A3 (es)
JP (1) JPS5879996A (es)
AU (1) AU8960982A (es)
DE (1) DE3141691A1 (es)
DK (1) DK466282A (es)
ES (1) ES516639A0 (es)
FI (1) FI823572L (es)
IL (1) IL67024A0 (es)
ZA (1) ZA827664B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4847200A (en) * 1983-11-25 1989-07-11 Queen's University At Kingston Biosynthesis of unnatural cephalosporins
US4753877A (en) * 1984-11-07 1988-06-28 Penalva Miguel A Cephalosporium acremonium
US4925794A (en) * 1985-01-02 1990-05-15 Fujisawa Pharmaceutical Co., Ltd. Hybrid plasmids and microorganisms containing them
US4952502A (en) * 1987-02-24 1990-08-28 Eli Lilly And Company Carbomycin biosynthetic gene, designated carG, for use in streptomyces and other organisms
US5236708A (en) * 1989-11-03 1993-08-17 Iowa State University Research Foundation, Inc. Reduced-protein subunit vaccine for swine dysentery
GB9226905D0 (en) * 1992-12-24 1993-02-17 Wellcome Found Pharmaceutical preparation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB938758A (en) * 1961-01-05 1963-10-09 Nat Res Dev Production of cephalosporin c.
EP0092388B2 (en) * 1982-04-16 1994-10-26 Eli Lilly And Company Cloning vectors

Also Published As

Publication number Publication date
FI823572A0 (fi) 1982-10-19
DK466282A (da) 1983-04-22
US4506014A (en) 1985-03-19
AU8960982A (en) 1983-04-28
IL67024A0 (en) 1983-02-23
EP0077557A3 (de) 1983-08-03
JPS5879996A (ja) 1983-05-13
ES516639A0 (es) 1983-07-16
FI823572L (fi) 1983-04-22
EP0077557A2 (de) 1983-04-27
DE3141691A1 (de) 1983-05-19
ZA827664B (en) 1983-08-31

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