ES470254A1 - A method of preparation of a transfer vector (Machine-translation by Google Translate, not legally binding) - Google Patents
A method of preparation of a transfer vector (Machine-translation by Google Translate, not legally binding)Info
- Publication number
- ES470254A1 ES470254A1 ES470254A ES470254A ES470254A1 ES 470254 A1 ES470254 A1 ES 470254A1 ES 470254 A ES470254 A ES 470254A ES 470254 A ES470254 A ES 470254A ES 470254 A1 ES470254 A1 ES 470254A1
- Authority
- ES
- Spain
- Prior art keywords
- transfer vector
- nucleotide sequence
- protein
- mrna
- translation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Abstract
A method of preparing a dna transfer vector with a nucleotide sequence for a specific protein, which method comprises: a) isolating cells containing mrnas that encode the protein from an organism that produces the protein. B) extracting the mrna from the cells in the presence of an rnase inhibitor composition, thereby preventing degradation of the rnase mrna; c) separating the mrna essentially free of protein, dna and other rna; d) synthesizing a double helix cdna, in which a strand contains a nucleotide sequence complementary to the mrna, thus producing a cdna with a nucleotide sequence encoding the desired protein; e) providing a dna transfer vector with reactive ends capable of being linked together or of being joined with double-stranded cdna, f) attaching the transfer vector of the adb with the double-stranded cdna containing a nucleotide sequence that it encodes insulin, thereby producing a dna transfer vector that contains a nucleotide sequence that encodes the desired protein. (Machine-translation by Google Translate, not legally binding)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US80134377A | 1977-05-27 | 1977-05-27 | |
US80502377A | 1977-06-09 | 1977-06-09 | |
US89770978A | 1978-04-19 | 1978-04-19 | |
US05897710 US4363877B1 (en) | 1977-09-23 | 1978-04-19 | Recombinant dna transfer vectors |
Publications (1)
Publication Number | Publication Date |
---|---|
ES470254A1 true ES470254A1 (en) | 1979-10-01 |
Family
ID=27505812
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES470254A Expired ES470254A1 (en) | 1977-05-27 | 1978-05-26 | A method of preparation of a transfer vector (Machine-translation by Google Translate, not legally binding) |
ES470253A Expired ES470253A1 (en) | 1977-05-27 | 1978-05-26 | A method to obtain a modified microorganism (Machine-translation by Google Translate, not legally binding) |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES470253A Expired ES470253A1 (en) | 1977-05-27 | 1978-05-26 | A method to obtain a modified microorganism (Machine-translation by Google Translate, not legally binding) |
Country Status (1)
Country | Link |
---|---|
ES (2) | ES470254A1 (en) |
-
1978
- 1978-05-26 ES ES470254A patent/ES470254A1/en not_active Expired
- 1978-05-26 ES ES470253A patent/ES470253A1/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES470253A1 (en) | 1979-09-16 |
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