ES468720A1 - Separating method of urokinase and medicin containg urokinase as useful component - Google Patents

Separating method of urokinase and medicin containg urokinase as useful component

Info

Publication number
ES468720A1
ES468720A1 ES468720A ES468720A ES468720A1 ES 468720 A1 ES468720 A1 ES 468720A1 ES 468720 A ES468720 A ES 468720A ES 468720 A ES468720 A ES 468720A ES 468720 A1 ES468720 A1 ES 468720A1
Authority
ES
Spain
Prior art keywords
urokinase
medicin
containg
contg
separating method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES468720A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Choay SA
Original Assignee
Choay SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Choay SA filed Critical Choay SA
Publication of ES468720A1 publication Critical patent/ES468720A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

Sepn. of a urokinase with a mol. wt. of ca. 54,000 (urokinase II) and a fraction contg. a urokinase with a mol. wt. of ca. 33,000 (urokinase I) from mixts. contg. them is effected by chromatographing a buffer soln. of the mixt. on an ion exchange resin, eluting with a concn. gradient of buffer salt. Also claimed is a pharmaceutical compsn. contg. urokinase II and 1 wt. % urokinase I. Since urokinase II has higher activity than I w.r.t. natural glutamyl-plasminogen and is equally active w.r.t. lysyl-plasminogen, it can be used as a thrombolytic agent at lower doses than conventional mixts. of I and II.
ES468720A 1977-04-12 1978-04-12 Separating method of urokinase and medicin containg urokinase as useful component Expired ES468720A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7711004A FR2387242A1 (en) 1977-04-12 1977-04-12 Purified thrombolytic urokinase products - isolated by ion-exchange chromatography

Publications (1)

Publication Number Publication Date
ES468720A1 true ES468720A1 (en) 1978-12-16

Family

ID=9189313

Family Applications (1)

Application Number Title Priority Date Filing Date
ES468720A Expired ES468720A1 (en) 1977-04-12 1978-04-12 Separating method of urokinase and medicin containg urokinase as useful component

Country Status (10)

Country Link
JP (1) JPS53130485A (en)
BE (1) BE865926A (en)
DE (1) DE2815853A1 (en)
DK (1) DK159878A (en)
ES (1) ES468720A1 (en)
FR (1) FR2387242A1 (en)
IT (1) IT1156188B (en)
LU (1) LU79426A1 (en)
NL (1) NL7803888A (en)
SE (1) SE7804141L (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5411219A (en) * 1977-06-25 1979-01-27 Green Cross Corp:The Thrombolytic agent and its preparation
CY1382A (en) * 1979-11-13 1987-12-18 Syed Shaukat Husain Isolation of plasminogen activators useful as therapeutic and diagnostic agents
JPS606191A (en) * 1983-06-02 1985-01-12 Wakamoto Pharmaceut Co Ltd Separation of low-molecular urokinase and high- molecular urokinase
DE3439980A1 (en) * 1984-11-02 1986-05-07 Behringwerke Ag, 3550 Marburg METHOD FOR CLEANING AND PASTEURIZING UROKINASE
DE3512910A1 (en) * 1985-04-11 1986-10-16 Behringwerke Ag, 3550 Marburg METHOD FOR CLEANING PLASMINOGEN ACTIVATORS
US20030040095A1 (en) * 2001-03-16 2003-02-27 Achille Arini Method for the production of pharmaceutically active recombinant proteins

Also Published As

Publication number Publication date
SE7804141L (en) 1978-10-13
LU79426A1 (en) 1978-11-28
DE2815853A1 (en) 1978-10-19
IT1156188B (en) 1987-01-28
NL7803888A (en) 1978-10-16
JPS53130485A (en) 1978-11-14
FR2387242A1 (en) 1978-11-10
BE865926A (en) 1978-10-12
IT7848859A0 (en) 1978-04-12
DK159878A (en) 1978-10-13

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