ES434100A1 - Procedure for the obtaining or enrichment of immunoglobulins igg, iga and igm from serum or plasma. (Machine-translation by Google Translate, not legally binding) - Google Patents

Procedure for the obtaining or enrichment of immunoglobulins igg, iga and igm from serum or plasma. (Machine-translation by Google Translate, not legally binding)

Info

Publication number
ES434100A1
ES434100A1 ES434100A ES434100A ES434100A1 ES 434100 A1 ES434100 A1 ES 434100A1 ES 434100 A ES434100 A ES 434100A ES 434100 A ES434100 A ES 434100A ES 434100 A1 ES434100 A1 ES 434100A1
Authority
ES
Spain
Prior art keywords
igm
iga
igg
serum
plasma
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES434100A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES434100A1 publication Critical patent/ES434100A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells

Abstract

Procedure for obtaining or enriching immunoglobulins igg, iga and igm (igg, iga, igm, immunoglobulins of classes g, a and m respectively), from serum or plasma, characterized in that a) from serum or defibrinated plasma is precipitated, with a short-chain primary alcohol, a crude fraction of immunoglobulins, b) the precipitate from step (a) is brought to a state of aqueous solution, and from it the euglobulins are precipitated by adjustment of low conductivity and a weakly acid ph value, c) of the supernatant portion of step (b) the proteolytic ferments are separated by adsorption in a mineral adsorbent, d) in the supernatant portion of step (c) is adsorb the predominant part of the igm, together with other proteins, in a hardly soluble phosphate, and the adsorbate is separated, e) from the adsorbate of step (d) a fraction, which contains igm, is desorbed, and the igm is enriched by precipitation with an alcohol pri short-chain marium, f) the precipitate from step (e) is brought to the state of aqueous solution, mixed with aluminum hydroxide for the removal of impurities, and again that, g) of the supernatant portion of the step (d), released from igm by adsorption, igg and iga are precipitated with a short-chain primary alcohol, and h) if desired, for the separation of igg and iga, the iga is it absorbs selectively in aluminum hydroxide. (Machine-translation by Google Translate, not legally binding)
ES434100A 1974-01-30 1975-01-24 Procedure for the obtaining or enrichment of immunoglobulins igg, iga and igm from serum or plasma. (Machine-translation by Google Translate, not legally binding) Expired ES434100A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19742404265 DE2404265C3 (en) 1974-01-30 1974-01-30 Method of delivering immunoglobulins

Publications (1)

Publication Number Publication Date
ES434100A1 true ES434100A1 (en) 1976-12-01

Family

ID=5906008

Family Applications (1)

Application Number Title Priority Date Filing Date
ES434100A Expired ES434100A1 (en) 1974-01-30 1975-01-24 Procedure for the obtaining or enrichment of immunoglobulins igg, iga and igm from serum or plasma. (Machine-translation by Google Translate, not legally binding)

Country Status (2)

Country Link
DE (1) DE2404265C3 (en)
ES (1) ES434100A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2658334B2 (en) * 1976-12-23 1980-06-04 Behringwerke Ag, 3550 Marburg Process for the production of immunoglobulin preparations with reduced complement binding and a pharmaceutical containing the same
DE3310150A1 (en) * 1983-03-21 1984-09-27 Lentia GmbH Chem. u. pharm. Erzeugnisse - Industriebedarf, 8000 München METHOD FOR PRODUCING A IGG IMMUNOGLOBULIN SOLUTION WITHOUT SIDE EFFECTS FOR THE INTRAVENOUS APPLICATION
JPS6112629A (en) * 1984-06-28 1986-01-21 Lion Corp Composition for oral cavity application
DE3927111C3 (en) * 1989-08-17 1994-09-01 Biotest Pharma Gmbh Process for the preparation of unmodified intravenous IgM and / or IgA-containing immunoglobulin preparations
DE4115910A1 (en) * 1991-05-15 1992-11-19 Schweigle Bernhard Dipl Chem Prodn. of native and stable immunoglobulin - by extraction form total globulin with aq. methanol at specified pH, to provide IgG as prophylactic agent
DE19600939C1 (en) 1996-01-12 1997-08-28 Immuno Ag Procedure for the separation of IgG and IgA

Also Published As

Publication number Publication date
DE2404265C3 (en) 1980-05-29
DE2404265A1 (en) 1975-07-31
DE2404265B2 (en) 1979-08-23

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