ES2556144B1 - Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado - Google Patents

Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado Download PDF

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Publication number
ES2556144B1
ES2556144B1 ES201400536A ES201400536A ES2556144B1 ES 2556144 B1 ES2556144 B1 ES 2556144B1 ES 201400536 A ES201400536 A ES 201400536A ES 201400536 A ES201400536 A ES 201400536A ES 2556144 B1 ES2556144 B1 ES 2556144B1
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Spain
Prior art keywords
sea urchin
procedure
bioassay
cryopreservation
embryos
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Active
Application number
ES201400536A
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English (en)
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ES2556144A1 (es
Inventor
Ricardo BEIRAS GARCÍA-SABELL
Juan BELLAS BEREIJO
Estefanía PAREDES ROSENDO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidade de Vigo
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Universidade de Vigo
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Priority to ES201400536A priority Critical patent/ES2556144B1/es
Publication of ES2556144A1 publication Critical patent/ES2556144A1/es
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Publication of ES2556144B1 publication Critical patent/ES2556144B1/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G01N33/186—Water using one or more living organisms, e.g. a fish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado. El procedimiento se basa en el uso de un protocolo de criopreservación para blástulas de 8 horas de erizo de mar para poder almacenar en nitrógeno líquido embriones para su uso fuera de la temporada de reproducción natural de esta especie. Se ha desarrollado además, un bioensayo ecotoxicológico con embriones de erizo de mar criopreservados para la evaluación de la contaminación marina. El procedimiento objeto de esta patente permite el uso durante todo el año de embriones de erizo de mar tanto en bioensayos ecotoxicológicos como con aplicaciones en la industria de la acuicultura.
ES201400536A 2014-07-07 2014-07-07 Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado Active ES2556144B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201400536A ES2556144B1 (es) 2014-07-07 2014-07-07 Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201400536A ES2556144B1 (es) 2014-07-07 2014-07-07 Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado

Publications (2)

Publication Number Publication Date
ES2556144A1 ES2556144A1 (es) 2016-01-13
ES2556144B1 true ES2556144B1 (es) 2016-07-14

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ES201400536A Active ES2556144B1 (es) 2014-07-07 2014-07-07 Procedimiento para la criopreservación de embriones de erizo de mar y bioensayo asociado

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ES (1) ES2556144B1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111587876A (zh) * 2020-05-25 2020-08-28 大连海洋大学 中间球海胆胚胎的快速玻璃化冷冻保存与复苏方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2057337C1 (ru) * 1991-09-24 1996-03-27 Александр Маркович Грищенко Способ определения токсичности окружающей среды

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111587876A (zh) * 2020-05-25 2020-08-28 大连海洋大学 中间球海胆胚胎的快速玻璃化冷冻保存与复苏方法
WO2021238039A1 (zh) * 2020-05-25 2021-12-02 大连海洋大学 中间球海胆胚胎的快速玻璃化冷冻保存与复苏方法
CN111587876B (zh) * 2020-05-25 2023-03-21 大连海洋大学 中间球海胆胚胎的快速玻璃化冷冻保存与复苏方法

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Publication number Publication date
ES2556144A1 (es) 2016-01-13

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