ES2537784B1 - Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program - Google Patents
Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program Download PDFInfo
- Publication number
- ES2537784B1 ES2537784B1 ES201300750A ES201300750A ES2537784B1 ES 2537784 B1 ES2537784 B1 ES 2537784B1 ES 201300750 A ES201300750 A ES 201300750A ES 201300750 A ES201300750 A ES 201300750A ES 2537784 B1 ES2537784 B1 ES 2537784B1
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- multiple wavelengths
- holographic
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- computer program
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- 238000000034 method Methods 0.000 title abstract 5
- 238000004590 computer program Methods 0.000 title abstract 3
- 238000000386 microscopy Methods 0.000 title abstract 2
- 238000001228 spectrum Methods 0.000 abstract 1
Classifications
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/08—Synthesising holograms, i.e. holograms synthesized from objects or objects from holograms
- G03H1/0866—Digital holographic imaging, i.e. synthesizing holobjects from holograms
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
- G03H2001/0447—In-line recording arrangement
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/22—Processes or apparatus for obtaining an optical image from holograms
- G03H1/2249—Holobject properties
- G03H2001/2263—Multicoloured holobject
- G03H2001/2271—RGB holobject
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/26—Processes or apparatus specially adapted to produce multiple sub- holograms or to obtain images from them, e.g. multicolour technique
- G03H1/2645—Multiplexing processes, e.g. aperture, shift, or wavefront multiplexing
- G03H2001/266—Wavelength multiplexing
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2222/00—Light sources or light beam properties
- G03H2222/10—Spectral composition
- G03H2222/17—White light
- G03H2222/18—RGB trichrome light
Abstract
Método de reconstrucción holográfico basado en microscopia sin lentes en línea con múltiples longitudes de onda, microscopio holográfico sin lentes en línea basado en múltiples longitudes de onda y programa de ordenador#El método comprende:#- iluminar, de manera simultánea, un objeto (O) con luz divergente que incluye tres o más longitudes de onda ({la}{sub,1} + ({la}{sub,2} + ({la}{sub,3}+ );#- registrar un holograma múltiple del objeto iluminado (O); y#- obtener, por reconstrucción digital, una imagen holográfica compuesta mediante un procesado digital de la información multiespectral incluida en el holograma múltiple relativa a las tres o más longitudes de onda ({la}{sub,1} + ({la}{sub,2} + ({la}{sub,3}+ ), que incluye realizar una mezcla ponderada en el dominio de Fourier de los espectros correspondientes a cada longitud de onda.#El microscopio está adaptado para implementar el método del primer aspecto.#El programa de ordenador implementa la reconstrucción digital del método de la invención.Holographic reconstruction method based on microscopy without in-line lenses with multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program # The method comprises: # - simultaneously illuminate an object (O ) with divergent light that includes three or more wavelengths ({la} {sub, 1} + ({la} {sub, 2} + ({la} {sub, 3} +…); # - register a multiple hologram of the illuminated object (O); and # - obtain, by digital reconstruction, a holographic image composed by digital processing of the multispectral information included in the multiple hologram relative to the three or more wavelengths ({la} {sub, 1 } + ({la} {sub, 2} + ({la} {sub, 3} +…), which includes performing a weighted mix in the Fourier domain of the spectra corresponding to each wavelength. # The microscope is adapted to implement the first aspect method. # The computer program implements the reconstruction of gital of the method of the invention.
Description
Claims (1)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300750A ES2537784B1 (en) | 2013-08-02 | 2013-08-02 | Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program |
PCT/ES2014/000135 WO2015015023A1 (en) | 2013-08-02 | 2014-07-25 | Holographic reconstruction method using lensless in-line microscopy with multiple wavelengths, holographic lensless in-line microscope using multiple wavelengths and computer program |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300750A ES2537784B1 (en) | 2013-08-02 | 2013-08-02 | Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2537784A1 ES2537784A1 (en) | 2015-06-12 |
ES2537784B1 true ES2537784B1 (en) | 2016-04-12 |
Family
ID=52431045
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201300750A Active ES2537784B1 (en) | 2013-08-02 | 2013-08-02 | Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program |
Country Status (2)
Country | Link |
---|---|
ES (1) | ES2537784B1 (en) |
WO (1) | WO2015015023A1 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR3036800B1 (en) * | 2015-05-28 | 2020-02-28 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | METHOD OF OBSERVING A SAMPLE |
FR3049348B1 (en) * | 2016-03-23 | 2023-08-11 | Commissariat Energie Atomique | METHOD FOR CHARACTERIZING A PARTICLE IN A SAMPLE |
FR3056749B1 (en) | 2016-09-28 | 2018-11-23 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | METHOD FOR NUMBERING LEUKOCYTES IN A SAMPLE |
WO2018064660A1 (en) | 2016-09-30 | 2018-04-05 | University Of Utah Research Foundation | Lensless imaging device |
EP3339963B1 (en) | 2016-12-21 | 2020-08-12 | IMEC vzw | An apparatus and a method for in-line holographic imaging |
CN111273533B (en) * | 2019-09-27 | 2021-08-06 | 中国工程物理研究院激光聚变研究中心 | Coaxial digital holographic automatic focusing method and system |
CN110836867A (en) * | 2019-10-18 | 2020-02-25 | 南京大学 | Non-lens holographic microscopic particle characterization method based on convolutional neural network |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3695744A (en) * | 1971-01-14 | 1972-10-03 | Rca Corp | Holographic multicolor technique |
US9767341B2 (en) * | 2010-12-14 | 2017-09-19 | The Regents Of The University Of California | Method and device for holographic opto-fluidic microscopy |
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2013
- 2013-08-02 ES ES201300750A patent/ES2537784B1/en active Active
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2014
- 2014-07-25 WO PCT/ES2014/000135 patent/WO2015015023A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2015015023A1 (en) | 2015-02-05 |
ES2537784A1 (en) | 2015-06-12 |
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