ES2527255B2 - Compuestos fluorescentes - Google Patents

Compuestos fluorescentes Download PDF

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Publication number
ES2527255B2
ES2527255B2 ES201300610A ES201300610A ES2527255B2 ES 2527255 B2 ES2527255 B2 ES 2527255B2 ES 201300610 A ES201300610 A ES 201300610A ES 201300610 A ES201300610 A ES 201300610A ES 2527255 B2 ES2527255 B2 ES 2527255B2
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ES
Spain
Prior art keywords
compounds
cell
fluorescent compounds
staining
cultures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES201300610A
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English (en)
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ES2527255A1 (es
Inventor
José Luis Mascareñas Cid
Eugenio Vazquez Sentis
José MARTINEZ COSTAS
Mateo Isidro Sanchez Lopez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidade de Santiago de Compostela
Original Assignee
Universidade de Santiago de Compostela
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidade de Santiago de Compostela filed Critical Universidade de Santiago de Compostela
Priority to ES201300610A priority Critical patent/ES2527255B2/es
Priority to PCT/ES2014/070503 priority patent/WO2014202814A1/es
Publication of ES2527255A1 publication Critical patent/ES2527255A1/es
Application granted granted Critical
Publication of ES2527255B2 publication Critical patent/ES2527255B2/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C255/00—Carboxylic acid nitriles
    • C07C255/49—Carboxylic acid nitriles having cyano groups bound to carbon atoms of six-membered aromatic rings of a carbon skeleton
    • C07C255/58—Carboxylic acid nitriles having cyano groups bound to carbon atoms of six-membered aromatic rings of a carbon skeleton containing cyano groups and singly-bound nitrogen atoms, not being further bound to other hetero atoms, bound to the carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C257/00—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines
    • C07C257/10—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines
    • C07C257/18—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines having carbon atoms of amidino groups bound to carbon atoms of six-membered aromatic rings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Plural Heterocyclic Compounds (AREA)

Abstract

Compuestos fluorescentes. La presente invención se dirige a los compuestos de formula I, a un kit que comprende dichos compuestos y a su aplicación como agentes de tinción. Dichos compuestos están diseñados para que sean capaces de internalizarse en la célula viva, acumularse selectivamente en las mitocondrias y teñirlas de forma preferente a otros orgánulos celulares. Además tienen una elevada fotoestabilidad. Adicionalmente, los compuestos de la invención permiten ser aplicados en cultivos celulares, tanto cultivos primarios como líneas celulares, bien en células vivas o bien fijadas con parafornaldehido, manteniendo la eficacia de la tinción y su selectividad, permitiendo su uso en experimentos de visualización en tiempo real.

Description

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Claims (1)

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ES201300610A 2013-06-20 2013-06-20 Compuestos fluorescentes Active ES2527255B2 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201300610A ES2527255B2 (es) 2013-06-20 2013-06-20 Compuestos fluorescentes
PCT/ES2014/070503 WO2014202814A1 (es) 2013-06-20 2014-06-19 Compuestos fluorescentes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201300610A ES2527255B2 (es) 2013-06-20 2013-06-20 Compuestos fluorescentes

Publications (2)

Publication Number Publication Date
ES2527255A1 ES2527255A1 (es) 2015-01-21
ES2527255B2 true ES2527255B2 (es) 2015-06-09

Family

ID=52103994

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201300610A Active ES2527255B2 (es) 2013-06-20 2013-06-20 Compuestos fluorescentes

Country Status (2)

Country Link
ES (1) ES2527255B2 (es)
WO (1) WO2014202814A1 (es)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011007038A2 (es) * 2009-07-15 2011-01-20 Universidade De Santiago De Compostela Uso de compuestos derivados de bis-benzamidinios como agentes fiuorogénícos para señalizar secuencias específicas de adn de doble cadena, y nuevos compuestos
ES2368276B2 (es) * 2010-04-27 2012-06-28 Universidad De Santiago De Compostela Compuestos derivados de bis-benzamidinios como agentes fluorogénicos para señalizar secuencias específicas de adn de doble cadena.
ES2396076B2 (es) * 2011-08-24 2013-12-05 Universidade De Santiago De Compostela Internalización y activación fotocontrolada de moléculas pequeñas capaces de unirse al adn de doble hebra.

Also Published As

Publication number Publication date
WO2014202814A1 (es) 2014-12-24
ES2527255A1 (es) 2015-01-21

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