ES2525195A1 - Lacasse with high redox potential, functional in blood, by means of directed evolution, method for the production thereof, and applications of same - Google Patents

Lacasse with high redox potential, functional in blood, by means of directed evolution, method for the production thereof, and applications of same Download PDF

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Publication number
ES2525195A1
ES2525195A1 ES201330222A ES201330222A ES2525195A1 ES 2525195 A1 ES2525195 A1 ES 2525195A1 ES 201330222 A ES201330222 A ES 201330222A ES 201330222 A ES201330222 A ES 201330222A ES 2525195 A1 ES2525195 A1 ES 2525195A1
Authority
ES
Spain
Prior art keywords
lacasse
applications
production
redox potential
blood
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES201330222A
Other languages
Spanish (es)
Other versions
ES2525195B1 (en
Inventor
Miguel Alcalde Galeote
Diana Mate Mate
David GONZALEZ PEREZ
Marcos Pita Martinez
Antonio Lopez De Lacey
Roland Ludwig
Roman Kittl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES201330222A priority Critical patent/ES2525195B1/en
Priority to PCT/ES2014/070113 priority patent/WO2014128325A1/en
Publication of ES2525195A1 publication Critical patent/ES2525195A1/en
Application granted granted Critical
Publication of ES2525195B1 publication Critical patent/ES2525195B1/en
Withdrawn - After Issue legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

The invention relates to a lacasse with high redox potential produced by means of directed molecular evolution, which is active in electrophysiological conditions, resists high halide concentrations, has a significant neutral/alkaline pH activity and is active in human blood and plasma. The invention relates to the amino-acidic sequence of said lacasse, to the nucleotide sequence encoding for said lacasse and cells that allow the production thereof. The lacasse according to the invention has applications in various sectors: nanobiotechnology, biomedicine, bioremediation processes, and paper and fine chemistry industries.

Description

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Claims (1)

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ES201330222A 2013-02-19 2013-02-19 HIGH POTENTIAL REDOX FUNCTIONAL IN BLOOD THROUGH DIRECT EVOLUTION METHOD OF OBTAINING AND ITS APPLICATIONS Withdrawn - After Issue ES2525195B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201330222A ES2525195B1 (en) 2013-02-19 2013-02-19 HIGH POTENTIAL REDOX FUNCTIONAL IN BLOOD THROUGH DIRECT EVOLUTION METHOD OF OBTAINING AND ITS APPLICATIONS
PCT/ES2014/070113 WO2014128325A1 (en) 2013-02-19 2014-02-14 Lacasse with high redox potential, functional in blood, by means of directed evolution, method for the production thereof, and applications of same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201330222A ES2525195B1 (en) 2013-02-19 2013-02-19 HIGH POTENTIAL REDOX FUNCTIONAL IN BLOOD THROUGH DIRECT EVOLUTION METHOD OF OBTAINING AND ITS APPLICATIONS

Publications (2)

Publication Number Publication Date
ES2525195A1 true ES2525195A1 (en) 2014-12-18
ES2525195B1 ES2525195B1 (en) 2015-09-24

Family

ID=51390533

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201330222A Withdrawn - After Issue ES2525195B1 (en) 2013-02-19 2013-02-19 HIGH POTENTIAL REDOX FUNCTIONAL IN BLOOD THROUGH DIRECT EVOLUTION METHOD OF OBTAINING AND ITS APPLICATIONS

Country Status (2)

Country Link
ES (1) ES2525195B1 (en)
WO (1) WO2014128325A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4174174A1 (en) 2021-10-29 2023-05-03 Consejo Superior de Investigaciones Cientificas Tailor-made extremophilic fungal laccases

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200948964A (en) * 2008-04-17 2009-12-01 Novozymes As Laccase variants
ES2370216B1 (en) * 2010-05-17 2012-10-19 Consejo Superior De Investigaciones Científicas (Csic) REDOX HIGH POTENTIAL LACASA.

Also Published As

Publication number Publication date
WO2014128325A1 (en) 2014-08-28
ES2525195B1 (en) 2015-09-24

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