ES2484665B2 - Electrode device for the detection of gluconic acid, manufacturing process and use of said device - Google Patents
Electrode device for the detection of gluconic acid, manufacturing process and use of said device Download PDFInfo
- Publication number
- ES2484665B2 ES2484665B2 ES201300149A ES201300149A ES2484665B2 ES 2484665 B2 ES2484665 B2 ES 2484665B2 ES 201300149 A ES201300149 A ES 201300149A ES 201300149 A ES201300149 A ES 201300149A ES 2484665 B2 ES2484665 B2 ES 2484665B2
- Authority
- ES
- Spain
- Prior art keywords
- detection
- electrode
- gluconic acid
- active area
- work
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La presente invención consiste en un dispositivo electródico para la detección de ácido glucónico, con tres electrodos, uno de referencia, otro de trabajo y otro auxiliar, obtenidos todos ellos por serigrafía, el área activa del electrodo de referencia incluye tinta de plata/cloruro de plata, la del contraelectrodo tinta de carbono y la del electrodo de trabajo con una tinta de carbono que incorpora tetratiafulvaleno (TTF) al 3%, sobre una lámina plástica de poliéster, modificándose el área activa del trabajo con la enzima GADH utilizando un agente bifuncional en presencia de seroalbúmina bovina (BSA), así como su procedimiento de fabricación y su uso en la detección citada, de manera simple, con bajo coste y en relativo corto tiempo de análisis.The present invention consists of an electrode device for the detection of gluconic acid, with three electrodes, one reference, one working and one auxiliary, all obtained by screen printing, the active area of the reference electrode includes silver ink / chloride silver, the carbon ink counter electrode and the work electrode with a carbon ink incorporating 3% tetrathiafulvalene (TTF) on a plastic polyester sheet, modifying the active area of work with the GADH enzyme using a bifunctional agent in the presence of bovine serum albumin (BSA), as well as its manufacturing procedure and its use in the detection mentioned, in a simple way, with low cost and in a relatively short time of analysis.
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300149A ES2484665B2 (en) | 2013-02-11 | 2013-02-11 | Electrode device for the detection of gluconic acid, manufacturing process and use of said device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300149A ES2484665B2 (en) | 2013-02-11 | 2013-02-11 | Electrode device for the detection of gluconic acid, manufacturing process and use of said device |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2484665A1 ES2484665A1 (en) | 2014-08-11 |
ES2484665B2 true ES2484665B2 (en) | 2015-03-16 |
Family
ID=51265999
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201300149A Active ES2484665B2 (en) | 2013-02-11 | 2013-02-11 | Electrode device for the detection of gluconic acid, manufacturing process and use of said device |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2484665B2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2638737B1 (en) * | 2016-04-22 | 2018-08-14 | Universidad De Burgos | Electrode device and measurement procedure for the detection of lactic acid |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001090733A1 (en) * | 2000-05-23 | 2001-11-29 | Radiometer Medical A/S | A sensor membrane, a method for the preparation thereof, a sensor and a layered membrane structure for such sensor |
DE60141214D1 (en) * | 2000-09-25 | 2010-03-18 | Asahi Chemical Ind | ENZYME ELECTRODE |
ATE350775T1 (en) * | 2000-11-09 | 2007-01-15 | Ird As | MEMBRANE ELECTRODE ARRANGEMENTS FOR DIRECT METHANOL FUEL CELLS AND METHOD FOR THE PRODUCTION THEREOF |
CN101802597B (en) * | 2007-09-18 | 2013-08-21 | 究极酵素国际股份有限公司 | Enzyme electrode |
-
2013
- 2013-02-11 ES ES201300149A patent/ES2484665B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
ES2484665A1 (en) | 2014-08-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2487268T3 (en) | Functional element with protection characteristics against rotation, as well as assembly piece formed by the functional element and a sheet metal part | |
ES2425316B1 (en) | Auxiliary wheelchair mobility system | |
ES2646949T3 (en) | Motorized transport roller | |
EP2874185A3 (en) | Organic light emitting display panel and organic light emitting display device including the same | |
MX2017013947A (en) | Tag layout for industrial vehicle operation. | |
CL2012001860E1 (en) | Industrial drawing applicable to the screen of a mobile device, made up of a vertical rectangle that has a central circle of width similar to the width of the screen, with a thin vertical rectangle on its right side that has five smaller circles inside. | |
AR073988A1 (en) | SYSTEM AND METHOD OF USER CONTROLS AND ITS CORRESPONDING LEGIBLE STORAGE BY COMPUTER | |
ES2639801T3 (en) | Mine for writing, drawing and / or painting instruments, as well as production procedure | |
BR112014027489A2 (en) | an electrode and its use | |
JP2019511757A5 (en) | ||
ES2667094T3 (en) | Airplane access tunnel | |
ES2567323T3 (en) | Polymer laser workpiece | |
TW201614629A (en) | Organic light-emitting display device | |
AR090178A1 (en) | PRINTING METHOD WITH OXIDATION DRY CALCOGRAPHIC INK AND UV-VIS CURABLE CALCOGRAPHIC INKS | |
CL2013002844S1 (en) | Car with a parallelepipedic body, which has an isoscelic trapezoidal bonnet, which in its frontal part of the vehicle and below the bonnet, has a first inverted isoscellic trapecial frontal recess arranged centered on said frontal strip, whose lower edge includes a square projection. | |
JP2014197184A5 (en) | ||
ES2484665B2 (en) | Electrode device for the detection of gluconic acid, manufacturing process and use of said device | |
CO2017001477A2 (en) | Graphotechnical test with obstacle to endorsing signatures with the naked eye | |
AR096937A1 (en) | SET OF DISPLAYATION DISPLAY AND DISPLAY | |
CO2018002064A2 (en) | Multilayer single-use ticket and redemption technique | |
AR105216A1 (en) | FRIENDLY INK AND FOUNTAIN SOLUTION WITH THE ENVIRONMENT FOR THE OFFSET PRINTING PROCESS IN WET AND OFFSET PRINTING PROCESS IN WET | |
ES2634088T3 (en) | Device system and procedure for use in machines for electrochemical pattern replication | |
CL2019002903A1 (en) | Graphic arts set with magnetic support structure. | |
ES2481450T3 (en) | Ubiquitin specific proteases responsible for the stability of mcl 1 and its use | |
AR096170A1 (en) | POINT FOR CAPACITIVE DISPLAY |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 2484665 Country of ref document: ES Kind code of ref document: B2 Effective date: 20150316 |