ES2484665B2 - Electrode device for the detection of gluconic acid, manufacturing process and use of said device - Google Patents

Electrode device for the detection of gluconic acid, manufacturing process and use of said device Download PDF

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Publication number
ES2484665B2
ES2484665B2 ES201300149A ES201300149A ES2484665B2 ES 2484665 B2 ES2484665 B2 ES 2484665B2 ES 201300149 A ES201300149 A ES 201300149A ES 201300149 A ES201300149 A ES 201300149A ES 2484665 B2 ES2484665 B2 ES 2484665B2
Authority
ES
Spain
Prior art keywords
detection
electrode
gluconic acid
active area
work
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES201300149A
Other languages
Spanish (es)
Other versions
ES2484665A1 (en
Inventor
Lorena DEL TORNO DE ROMÁN
María Asunción ALONSO LOMILLO
Olga DOMÍNGUEZ RENEDO
Arrate JAUREGUIBEITIA
María Julia ARCOS MARTÍNEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Burgos
Original Assignee
Universidad de Burgos
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Universidad de Burgos filed Critical Universidad de Burgos
Priority to ES201300149A priority Critical patent/ES2484665B2/en
Publication of ES2484665A1 publication Critical patent/ES2484665A1/en
Application granted granted Critical
Publication of ES2484665B2 publication Critical patent/ES2484665B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invención consiste en un dispositivo electródico para la detección de ácido glucónico, con tres electrodos, uno de referencia, otro de trabajo y otro auxiliar, obtenidos todos ellos por serigrafía, el área activa del electrodo de referencia incluye tinta de plata/cloruro de plata, la del contraelectrodo tinta de carbono y la del electrodo de trabajo con una tinta de carbono que incorpora tetratiafulvaleno (TTF) al 3%, sobre una lámina plástica de poliéster, modificándose el área activa del trabajo con la enzima GADH utilizando un agente bifuncional en presencia de seroalbúmina bovina (BSA), así como su procedimiento de fabricación y su uso en la detección citada, de manera simple, con bajo coste y en relativo corto tiempo de análisis.The present invention consists of an electrode device for the detection of gluconic acid, with three electrodes, one reference, one working and one auxiliary, all obtained by screen printing, the active area of the reference electrode includes silver ink / chloride silver, the carbon ink counter electrode and the work electrode with a carbon ink incorporating 3% tetrathiafulvalene (TTF) on a plastic polyester sheet, modifying the active area of work with the GADH enzyme using a bifunctional agent in the presence of bovine serum albumin (BSA), as well as its manufacturing procedure and its use in the detection mentioned, in a simple way, with low cost and in a relatively short time of analysis.

Description

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Claims (1)

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ES201300149A 2013-02-11 2013-02-11 Electrode device for the detection of gluconic acid, manufacturing process and use of said device Active ES2484665B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201300149A ES2484665B2 (en) 2013-02-11 2013-02-11 Electrode device for the detection of gluconic acid, manufacturing process and use of said device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201300149A ES2484665B2 (en) 2013-02-11 2013-02-11 Electrode device for the detection of gluconic acid, manufacturing process and use of said device

Publications (2)

Publication Number Publication Date
ES2484665A1 ES2484665A1 (en) 2014-08-11
ES2484665B2 true ES2484665B2 (en) 2015-03-16

Family

ID=51265999

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201300149A Active ES2484665B2 (en) 2013-02-11 2013-02-11 Electrode device for the detection of gluconic acid, manufacturing process and use of said device

Country Status (1)

Country Link
ES (1) ES2484665B2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2638737B1 (en) * 2016-04-22 2018-08-14 Universidad De Burgos Electrode device and measurement procedure for the detection of lactic acid

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1292823A1 (en) * 2000-05-23 2003-03-19 Radiometer Medical A/S A sensor membrane, a method for the preparation thereof, a sensor and a layered membrane structure for such sensor
CN1198138C (en) * 2000-09-25 2005-04-20 旭化成株式会社 Enzyme electrode
AU2002224846A1 (en) * 2000-11-09 2002-05-21 Ird A/S Membrane electrode assemblies for direct methanol fuel cells and methods for their production
RU2476869C2 (en) * 2007-09-18 2013-02-27 Алтизайм Интернэшнл Лтд Enzyme electrode

Also Published As

Publication number Publication date
ES2484665A1 (en) 2014-08-11

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