ES2479895A1 - Double-recognition immunoassay for antibody detection (Machine-translation by Google Translate, not legally binding) - Google Patents

Double-recognition immunoassay for antibody detection (Machine-translation by Google Translate, not legally binding) Download PDF

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Publication number
ES2479895A1
ES2479895A1 ES200931127A ES200931127A ES2479895A1 ES 2479895 A1 ES2479895 A1 ES 2479895A1 ES 200931127 A ES200931127 A ES 200931127A ES 200931127 A ES200931127 A ES 200931127A ES 2479895 A1 ES2479895 A1 ES 2479895A1
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Spain
Prior art keywords
antigen
translation
double
machine
target antigen
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ES200931127A
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Spanish (es)
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ES2479895B1 (en
ES2479895B8 (en
Inventor
Carmen Vela Olmo
Ángel Antonio VENTEO MORENO
Antonio José SANZ FERNÁNDEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inmunologia y Genetica Aplicada SA
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Inmunologia y Genetica Aplicada SA
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Priority to ES200931127A priority Critical patent/ES2479895B8/en
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The double-recognition immunoassay for the detection of specific antibodies of a target antigen in a sample, wherein the target antigen is a virus of the genus arterivirus and in particular, the porcine reproductive and respiratory syndrome virus (prrsv), comprises contacting said target antigen with a sample suspected of containing said specific antibodies of said target antigen under conditions that allow the formation of an antigen-antibody complex; adding a conjugate comprising said target antigen and a label under conditions that allow the formation of an antigen-antibody-antigen/label complex; and detecting said antigen-antibody-antigen/marker complex. The immunoassay can be used, among other applications, in the diagnosis of infections caused by said virus with high sensitivity and specificity. (Machine-translation by Google Translate, not legally binding)

Description

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Claims (1)

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ES200931127A 2009-12-07 2009-12-07 Dual recognition immunoassay for antibody detection Active ES2479895B8 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200931127A ES2479895B8 (en) 2009-12-07 2009-12-07 Dual recognition immunoassay for antibody detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200931127A ES2479895B8 (en) 2009-12-07 2009-12-07 Dual recognition immunoassay for antibody detection

Publications (3)

Publication Number Publication Date
ES2479895A1 true ES2479895A1 (en) 2014-07-24
ES2479895B1 ES2479895B1 (en) 2015-07-09
ES2479895B8 ES2479895B8 (en) 2015-08-05

Family

ID=51211439

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200931127A Active ES2479895B8 (en) 2009-12-07 2009-12-07 Dual recognition immunoassay for antibody detection

Country Status (1)

Country Link
ES (1) ES2479895B8 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006093797A2 (en) * 2005-02-28 2006-09-08 Mj Biologics, Inc. Method and kit for detecting porcine reproductive and respiratory syndrome virus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006093797A2 (en) * 2005-02-28 2006-09-08 Mj Biologics, Inc. Method and kit for detecting porcine reproductive and respiratory syndrome virus

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN S et al. Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum.European Journal of Clinical Microbiology & Infectious Diseases, 20050801 Springer, Berlin, DE 01/08/2005 VOL: 24 No: 8 Pags: 549 - 553 ISSN 1435-4373 Doi: doi:10.1007/s10096-005-1378-7 *
FERRIN NEAL H et al. Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus.Clinical and Diagnostic Laboratory Immunology May 2004 00/05/2004 VOL: 11 No: 3 Pags: 503-514 ISSN 1071-412X (ISSN print) *
SEUBERLICH T et al. Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Detection and Differentiation of Antibodies Against European and North American Porcine Reproductive and Respiratory Syndrome Virus.CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 20021101 AMERICAN SOCIETY FOR MICROBIOLOGY, US 01/11/2002 VOL: 9 No: 6 Pags: 1183 - 1191 ISSN 1071-412X Doi: doi:10.1128/CDLI.9.6.1183-1191.2002 *

Also Published As

Publication number Publication date
ES2479895B1 (en) 2015-07-09
ES2479895B8 (en) 2015-08-05

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