ES2445467B1 - VACCINE AGAINST INFESTATIONS CAUSED BY HEMATOPHAGUS ARTHROPODS - Google Patents

VACCINE AGAINST INFESTATIONS CAUSED BY HEMATOPHAGUS ARTHROPODS Download PDF

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ES2445467B1
ES2445467B1 ES201231253A ES201231253A ES2445467B1 ES 2445467 B1 ES2445467 B1 ES 2445467B1 ES 201231253 A ES201231253 A ES 201231253A ES 201231253 A ES201231253 A ES 201231253A ES 2445467 B1 ES2445467 B1 ES 2445467B1
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arthropods
vaccine against
hematophagus
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infestations caused
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ES2445467A1 (en
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José De Jesús DE LA FUENTE GARCÍA
Margarita VILLAR RAYO
Carlos Roberto PRUDENCIO
José Manuel PÉREZ DE LA LASTRA
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Universidad de Castilla La Mancha
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Universidad de Castilla La Mancha
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0003—Invertebrate antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43527—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from ticks
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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Abstract

La presente invención describe una vacuna frente a infestaciones provocadas por artrópodos hematófagos preferentemente garrapatas, mosquitos, flebótomos, ácaros rojos de las gallinas, pulgas, piojos, piojos de mar, etc. Así mismo, se protege un péptido capaz de inducir una respuesta inmune protectora y de reacción cruzada frente a una infestación provocada por artrópodos hematófagos, un polinucleótido, un vector de expresión, una célula hospedadora, un animal no humano, un anticuerpo, y un medicamento, así como el uso de estos productos en medicina, preferentemente en tecnologías del ADN recombinante y en inmunología.The present invention describes a vaccine against infestations caused by hematophagous arthropods, preferably ticks, mosquitoes, sandflies, red chicken mites, fleas, lice, sea lice, etc. Likewise, a peptide capable of inducing a protective and cross-reactive immune response against an infestation caused by hematophagous arthropods, a polynucleotide, an expression vector, a host cell, a non-human animal, an antibody, and a medicament is protected. , as well as the use of these products in medicine, preferably in recombinant DNA technologies and in immunology.

Description

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0.55 0.55
0.43 0.42 0.62 0.56 0.43 0.42 0.62 0.56

0.56 0.56
0.28 0.41 0.82 0.51 0.28 0.41 0.82 0.51

0.95 0.95
ND 0.70 0.38 0.66 ND 0.70 0.38 0.66

(0.71±0.17) (0.71 ± 0.17)
(0.37±0.7)* § (0.52±0.11)* § (0.51±0.18)* § (0.61±0.07)* (0.37 ± 0.7) * § (0.52 ± 0.11) * § (0.51 ± 0.18) * § (0.61 ± 0.07) *

Aedes albopictus Aedes albopictus

Mortalidad de 0.00 0.00 mortality
0.10 0.20 0.00 0.05 0.10 0.20 0.00 0.05

hembras 0.25 females 0.25
0.20 0.00 0.15 0.30 0.20 0.00 0.15 0.30

(Nº muertas/hembras 0.10 (No. dead / females 0.10
0.10 0.05 0.10 0.10 0.10 0.05 0.10 0.10

alimentadas) 0.10 fed) 0.10
0.15 0.10 0.05 0.25 0.15 0.10 0.05 0.25

0.10 0.10
0.15 0.05 0.00 0.30 0.15 0.05 0.00 0.30

0.10 0.10
0.10 0.10 0.10 0.30 0.10 0.10 0.10 0.30

(0.11±0.08) (0.11 ± 0.08)
(0.13±0.04) (0.08±0.07) (0.07±0.06) (0.22±0.11)* § (0.13 ± 0.04) (0.08 ± 0.07) (0.07 ± 0.06) (0.22 ± 0.11) * §

Oviposición 42 Oviposition 42
49 55 41 57 49 55 41 57

(Nº huevos/hembra) 35 (No. eggs / female) 35
50 59 35 51 50 59 35 51

32 32
51 38 25 54 51 38 25 54

49 49
33 49 8 55 33 49 8 55

41 41
36 51 37 53 36 51 37 53

61 61
69 53 40 38 69 53 40 38

(43±10) (43 ± 10)
(48±13) (51±7) (31±13)* § (51±7) (48 ± 13) (51 ± 7) (31 ± 13) * § (51 ± 7)

Fertilidad 1 0.38 Fertility 1 0.38
0.10 0.07 0.21 0.02 0.10 0.07 0.21 0.02

(Nº larvas/huevo) 0.23 (No. larvae / egg) 0.23
0.01 0.06 0.29 0.002 0.01 0.06 0.29 0.002

0.37 0.37
0.00 0.06 0.27 0.03 0.00 0.06 0.27 0.03

0.002 0.002
0.11 0.02 0.79 0.02 0.11 0.02 0.79 0.02

0.45 0.45
0.07 001 0.25 0.06 0.07 001 0.25 0.06

0.07 0.07
0.02 0.004 0.23 0.01 0.02 0.004 0.23 0.01

(0.25±0.18) (0.25 ± 0.18)
(0.05±0.05)* § (0.04±0.03)* § (0.34±0.22) (0.02±0.02)* § (0.05 ± 0.05) * § (0.04 ± 0.03) * § (0.34 ± 0.22) (0.02 ± 0.02) * §

Fertilidad 2 0.18 Fertility 2 0.18
0.08 0.05 0.05 0.02 0.08 0.05 0.05 0.02

(Nº adultos/huevo) 0.10 (No. adults / egg) 0.10
0.00 0.04 0.07 0.00 0.00 0.04 0.07 0.00

0.18 0.18
0.00 0.04 0.14 0.02 0.00 0.04 0.14 0.02

0.00 0.00
0.10 0.01 0.74 0.01 0.10 0.01 0.74 0.01

0.20 0.20
0.07 0.00 0.10 0.05 0.07 0.00 0.10 0.05

0.06 0.06
0.02 0.00 0.12 0.01 0.02 0.00 0.12 0.01

(0.12±0.08) (0.12 ± 0.08)
(0.05±0.04)* § (0.02±0.02)* § (0.20±0.26) (0.02±0.02)* § (0.05 ± 0.04) * § (0.02 ± 0.02) * § (0.20 ± 0.26) (0.02 ± 0.02) * §

Phlebotomus perniciosus Phlebotomus perniciosus

Mortalidad de 0.00 0.00 mortality
0.50 0.40 0.80 0.70 0.50 0.40 0.80 0.70

hembras 0.30 females 0.30
0.40 0.30 0.50 0.30 0.40 0.30 0.50 0.30

(Nº muertas/hembras 0.40 (No. dead / females 0.40
0.30 0.40 0.30 0.20 0.30 0.40 0.30 0.20

alimentadas) 0.30 fed) 0.30
0.50 0.20 0.20 0.20 0.50 0.20 0.20 0.20

0.30 0.30
0.30 0.40 0.30 0.60 0.30 0.40 0.30 0.60

0.30 0.30
0.50 0.40 0.30 0.30 0.50 0.40 0.30 0.30

(0.27±0.14) (0.27 ± 0.14)
(0.42±0.10) § (0.35±0.08) (0.40±0.22) (0.38±0.21) (0.42 ± 0.10) § (0.35 ± 0.08) (0.40 ± 0.22) (0.38 ± 0.21)

Oviposición 28 Oviposition 28
15 4 6 7 15 4 6 7

(Nº huevos/hembra) 24 (No. eggs / female) 24
16 15 6 18 16 15 6 18

17 17
23 17 15 19 23 17 15 19

16 16
9 22 20 22 9 22 20 22

17 17
20 21 14 10 20 21 14 10

14 14
13 14 22 16 13 14 22 16

(19±6) (19 ± 6)
(16±5)* (16±6)* (14±7)* (15±6)* (16 ± 5) * (16 ± 6) * (14 ± 7) * (15 ± 6) *

Fertilidad 2 0.41 Fertility 2 0.41
0.20 0.68 0.52 0.67 0.20 0.68 0.52 0.67

(Nº adultos/huevo) 0.33 (No. adults / egg) 0.33
0.17 0.31 0.53 0.53 0.17 0.31 0.53 0.53

0.43 0.43
0.14 0.75 0.44 0.28 0.14 0.75 0.44 0.28

0.53 0.53
0.25 0.62 0.15 0.61 0.25 0.62 0.15 0.61

0.45 0.45
0.14 0.37 0.62 0.29 0.14 0.37 0.62 0.29

0.52 0.52
0.20 0.37 0.27 0.51 0.20 0.37 0.27 0.51

(0.44±0.08) (0.44 ± 0.08)
(0.18±0.04)* § (0.52±0.19) (0.42±0.18) (0.48±0.16) (0.18 ± 0.04) * § (0.52 ± 0.19) (0.42 ± 0.18) (0.48 ± 0.16)

Los resultados se muestran para cada ratón infestado con la media±S.D en paréntesis. Los datos fueron analizados estadísticamente para comparar los resultados para cada especie entre los vectores alimentados en ratones vacunados y del grupo control mediante el test Chi2 (*p<0.005) y de Student (§p<0.05). ND, sin The results are shown for each mouse infested with the mean ± S.D in parentheses. The data were statistically analyzed to compare the results for each species between the vectors fed in vaccinated mice and the control group using the Chi2 (* p <0.005) and Student (§p <0.05) test. ND, without

5 determinar debido a la muerte del ratón. 5 determine due to mouse death.

1.4. Discusión 1.4. Discussion

La presente invención muestra el efecto de la vacunación con quimeras de SUB/AK Q38 y Q41 en infestaciones 10 por garrapatas, mosquitos y mosquitos de arena (flebótomos), con efectos en la muda, oviposición y/o en The present invention shows the effect of vaccination with SUB / AK chimeras Q38 and Q41 in infestations 10 by ticks, mosquitoes and sand mosquitoes (sandflies), with effects on molting, oviposition and / or

13 13

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Claims (1)

imagen1image 1 imagen2image2
ES201231253A 2012-08-01 2012-08-01 VACCINE AGAINST INFESTATIONS CAUSED BY HEMATOPHAGUS ARTHROPODS Active ES2445467B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201231253A ES2445467B1 (en) 2012-08-01 2012-08-01 VACCINE AGAINST INFESTATIONS CAUSED BY HEMATOPHAGUS ARTHROPODS
PCT/ES2013/070560 WO2014020218A1 (en) 2012-08-01 2013-07-30 Vaccine against infestations caused by blood-sucking arthropods

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201231253A ES2445467B1 (en) 2012-08-01 2012-08-01 VACCINE AGAINST INFESTATIONS CAUSED BY HEMATOPHAGUS ARTHROPODS

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ES2445467A1 ES2445467A1 (en) 2014-03-03
ES2445467B1 true ES2445467B1 (en) 2014-12-12

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NO345611B1 (en) * 2016-01-22 2021-05-10 Norifish As IgY antibody composition, and use of the composition for reduction of infestation and infection by a Caligidae

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* Cited by examiner, † Cited by third party
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WO2009146513A1 (en) * 2008-06-03 2009-12-10 Vallée S.A. Immunogens for controlling bovine tick

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