ES2438391B1 - CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE - Google Patents

CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE Download PDF

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Publication number
ES2438391B1
ES2438391B1 ES201230911A ES201230911A ES2438391B1 ES 2438391 B1 ES2438391 B1 ES 2438391B1 ES 201230911 A ES201230911 A ES 201230911A ES 201230911 A ES201230911 A ES 201230911A ES 2438391 B1 ES2438391 B1 ES 2438391B1
Authority
ES
Spain
Prior art keywords
method associated
study
cell culture
different
cellular culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES201230911A
Other languages
Spanish (es)
Other versions
ES2438391A1 (en
Inventor
Luis José Fernández Ledesma
Ignacio OCHOA GARRIDO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Zaragoza
Centro de Investigacion Biomedica en Red en Bioingenieria Biomateriales y Nanomedicina CIBERBBN
Original Assignee
Universidad de Zaragoza
Centro de Investigacion Biomedica en Red en Bioingenieria Biomateriales y Nanomedicina CIBERBBN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Zaragoza, Centro de Investigacion Biomedica en Red en Bioingenieria Biomateriales y Nanomedicina CIBERBBN filed Critical Universidad de Zaragoza
Priority to ES201230911A priority Critical patent/ES2438391B1/en
Priority to PCT/ES2013/000141 priority patent/WO2013186408A1/en
Publication of ES2438391A1 publication Critical patent/ES2438391A1/en
Application granted granted Critical
Publication of ES2438391B1 publication Critical patent/ES2438391B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/80—Forming a predetermined ratio of the substances to be mixed
    • B01F35/81—Forming mixtures with changing ratios or gradients
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Dispositivo de cultivo celular y método asociado a dicho dispositivo.#La presente invención se refiere a un dispositivo encapsulable destinado para su uso en el estudio de cultivos celulares, y compuesto preferentemente de un material plástico donde se localizan diferentes pocillos para el cultivo celular, en el fondo de los cuales se deposita un material gelificado con propiedades mecánicas diferentes en cada pocillo. la invención tiene por objeto, preferentemente, la obtención de sistemas de análisis a gran escala que permitan hacer el estudio de la respuesta de un determinado tipo de células, fármacos, etc. en función de diferentes valores de rigidez del sustrato empleados para cada cultivo.Cell culture device and method associated with said device. # The present invention relates to an encapsulable device intended for use in the study of cell cultures, and preferably composed of a plastic material where different wells for cell culture are located, in the bottom of which a gelled material with different mechanical properties is deposited in each well. The object of the invention is preferably to obtain large-scale analysis systems that allow the study of the response of a certain type of cells, drugs, etc. depending on different values of substrate stiffness used for each crop.

Description



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Claims (1)



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ES201230911A 2012-06-12 2012-06-12 CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE Expired - Fee Related ES2438391B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201230911A ES2438391B1 (en) 2012-06-12 2012-06-12 CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE
PCT/ES2013/000141 WO2013186408A1 (en) 2012-06-12 2013-06-10 Cell culture device and method associated with said

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201230911A ES2438391B1 (en) 2012-06-12 2012-06-12 CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE

Publications (2)

Publication Number Publication Date
ES2438391A1 ES2438391A1 (en) 2014-01-16
ES2438391B1 true ES2438391B1 (en) 2014-10-22

Family

ID=49757625

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201230911A Expired - Fee Related ES2438391B1 (en) 2012-06-12 2012-06-12 CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE

Country Status (2)

Country Link
ES (1) ES2438391B1 (en)
WO (1) WO2013186408A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018067802A1 (en) * 2016-10-07 2018-04-12 The Regents Of The University Of Michigan Systems and methods for high throughput screening
CN110732355B (en) * 2019-09-27 2021-09-28 东南大学 Micro-mixing micro-fluidic chip

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0426809D0 (en) * 2004-12-07 2005-01-12 Univ Southampton Nano-optical diffraction bar-code tagging for biological and chemical applications
ES2263400B1 (en) * 2006-05-22 2007-08-16 Ikerlan Centro De Investigaciones Tecnologicas, S. Coop. FLEXIBLE MICRO-NANOFLUIDIC DEVICES.
WO2010024779A1 (en) * 2008-08-27 2010-03-04 Agency For Science, Technology And Research Microfluidic continuous flow device for culturing biological material
WO2012040579A2 (en) * 2010-09-23 2012-03-29 President And Fellows Of Harvard College Molecular screening across defined stiffness matrices

Also Published As

Publication number Publication date
WO2013186408A8 (en) 2014-02-06
ES2438391A1 (en) 2014-01-16
WO2013186408A1 (en) 2013-12-19

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