ES2388255B1 - TRANSCRIPTIONAL FUSION VECTORS FOR UNI- AND BIDIRECTIONAL PROMOTING REGIONS FOR USE IN LACTIC BACTERIA. - Google Patents

TRANSCRIPTIONAL FUSION VECTORS FOR UNI- AND BIDIRECTIONAL PROMOTING REGIONS FOR USE IN LACTIC BACTERIA.

Info

Publication number
ES2388255B1
ES2388255B1 ES201130356A ES201130356A ES2388255B1 ES 2388255 B1 ES2388255 B1 ES 2388255B1 ES 201130356 A ES201130356 A ES 201130356A ES 201130356 A ES201130356 A ES 201130356A ES 2388255 B1 ES2388255 B1 ES 2388255B1
Authority
ES
Spain
Prior art keywords
uni
bidirectional
fusion vectors
lactic bacteria
transcriptional fusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES201130356A
Other languages
Spanish (es)
Other versions
ES2388255A1 (en
Inventor
Cayuela Tomas Garcia
Bonillo M Luz Mohedano
Gomez De Cadinanos M Luz Perez
De Palencia Delgado Pilar Fernandez
Daniel Boden
Jerry Wells
Martinez Carmen Pelaez
Garcia Paloma Lopez
Rolania Teresa Requena
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES201130356A priority Critical patent/ES2388255B1/en
Priority to PCT/ES2012/070163 priority patent/WO2012123610A1/en
Publication of ES2388255A1 publication Critical patent/ES2388255A1/en
Application granted granted Critical
Publication of ES2388255B1 publication Critical patent/ES2388255B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES201130356A 2011-03-15 2011-03-15 TRANSCRIPTIONAL FUSION VECTORS FOR UNI- AND BIDIRECTIONAL PROMOTING REGIONS FOR USE IN LACTIC BACTERIA. Expired - Fee Related ES2388255B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201130356A ES2388255B1 (en) 2011-03-15 2011-03-15 TRANSCRIPTIONAL FUSION VECTORS FOR UNI- AND BIDIRECTIONAL PROMOTING REGIONS FOR USE IN LACTIC BACTERIA.
PCT/ES2012/070163 WO2012123610A1 (en) 2011-03-15 2012-03-14 Transcriptional fusion vectors for uni and bidirectional promoter regions for use in lactic bacteria

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201130356A ES2388255B1 (en) 2011-03-15 2011-03-15 TRANSCRIPTIONAL FUSION VECTORS FOR UNI- AND BIDIRECTIONAL PROMOTING REGIONS FOR USE IN LACTIC BACTERIA.

Publications (2)

Publication Number Publication Date
ES2388255A1 ES2388255A1 (en) 2012-10-11
ES2388255B1 true ES2388255B1 (en) 2013-09-13

Family

ID=46830083

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201130356A Expired - Fee Related ES2388255B1 (en) 2011-03-15 2011-03-15 TRANSCRIPTIONAL FUSION VECTORS FOR UNI- AND BIDIRECTIONAL PROMOTING REGIONS FOR USE IN LACTIC BACTERIA.

Country Status (2)

Country Link
ES (1) ES2388255B1 (en)
WO (1) WO2012123610A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8679749B2 (en) * 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation

Also Published As

Publication number Publication date
ES2388255A1 (en) 2012-10-11
WO2012123610A1 (en) 2012-09-20

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ES2388255B1 (en) TRANSCRIPTIONAL FUSION VECTORS FOR UNI- AND BIDIRECTIONAL PROMOTING REGIONS FOR USE IN LACTIC BACTERIA.

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