ES2357821B8 - METHOD FOR DETECTION AND DETERMINATION OF VIRAL CARGO OF THE HUMAN PAPILOMA VIRUS. - Google Patents

METHOD FOR DETECTION AND DETERMINATION OF VIRAL CARGO OF THE HUMAN PAPILOMA VIRUS. Download PDF

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Publication number
ES2357821B8
ES2357821B8 ES200930425A ES200930425A ES2357821B8 ES 2357821 B8 ES2357821 B8 ES 2357821B8 ES 200930425 A ES200930425 A ES 200930425A ES 200930425 A ES200930425 A ES 200930425A ES 2357821 B8 ES2357821 B8 ES 2357821B8
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Spain
Prior art keywords
determination
detection
hpv
amplifying
fragment
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200930425A
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Spanish (es)
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ES2357821B1 (en
ES2357821A1 (en
Inventor
Marcos Egea Gutierrez-Cortines
Julia Weis
Carles Iniesta Navalon
Vicente Santaclara Maneiro
Pablo Conesa Zamora
Miguel Perez-Guillermo Garcia
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Fundacion para la Formacion e Investigacion Sanitarias de la Region de Murcia
Universidad Politecnica de Cartagena
Original Assignee
Fundacion para la Formacion e Investigacion Sanitarias de la Region de Murcia
Universidad Politecnica de Cartagena
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Priority to ES200930425A priority Critical patent/ES2357821B8/en
Publication of ES2357821A1 publication Critical patent/ES2357821A1/en
Application granted granted Critical
Publication of ES2357821B1 publication Critical patent/ES2357821B1/en
Publication of ES2357821B8 publication Critical patent/ES2357821B8/en
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para la detección y determinación de carga viral del virus del papiloma humano.#Un par de cebadores degenerados capaces de amplificar un fragmento del gen L1 de varios genotipos del virus del papiloma humano (VPH) y un método de detección y de cuantificación de la carga viral del VPH en muestras biológicas aisladas, que comprende: la extracción de ADN de una muestra biológica aislada, poner en contacto la muestra con una mezcla de reacción que contiene al menos un fluorocromo y los cebadores degenerados de la invención, amplificar un fragmento del gen L1 del VPH, medir la fluorescencia obtenida en el paso de amplificación y comparar el valor de fluorescencia obtenido con una secuencia control.Method for the detection and determination of viral load of the human papillomavirus. # A pair of degenerated primers capable of amplifying a fragment of the L1 gene of several genotypes of the human papillomavirus (HPV) and a method of detecting and quantifying the HPV viral load in isolated biological samples, comprising: extracting DNA from an isolated biological sample, contacting the sample with a reaction mixture containing at least one fluorochrome and the degenerated primers of the invention, amplifying a fragment of the HPV L1 gene, measure the fluorescence obtained in the amplification step and compare the fluorescence value obtained with a control sequence.

ES200930425A 2009-07-07 2009-07-07 METHOD FOR DETECTION AND DETERMINATION OF VIRAL CARGO OF THE HUMAN PAPILOMA VIRUS. Expired - Fee Related ES2357821B8 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200930425A ES2357821B8 (en) 2009-07-07 2009-07-07 METHOD FOR DETECTION AND DETERMINATION OF VIRAL CARGO OF THE HUMAN PAPILOMA VIRUS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200930425A ES2357821B8 (en) 2009-07-07 2009-07-07 METHOD FOR DETECTION AND DETERMINATION OF VIRAL CARGO OF THE HUMAN PAPILOMA VIRUS.

Publications (3)

Publication Number Publication Date
ES2357821A1 ES2357821A1 (en) 2011-05-03
ES2357821B1 ES2357821B1 (en) 2012-01-13
ES2357821B8 true ES2357821B8 (en) 2012-03-05

Family

ID=43859198

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200930425A Expired - Fee Related ES2357821B8 (en) 2009-07-07 2009-07-07 METHOD FOR DETECTION AND DETERMINATION OF VIRAL CARGO OF THE HUMAN PAPILOMA VIRUS.

Country Status (1)

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ES (1) ES2357821B8 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69806024T2 (en) * 1997-09-16 2003-02-13 Innogenetics Nv DETECTION AND IDENTIFICATION OF HUMAN PAPILLOMAVIRUS BY PCR AND TYPE-SPECIFIC REVERSE HYBRIDIZATION

Also Published As

Publication number Publication date
ES2357821B1 (en) 2012-01-13
ES2357821A1 (en) 2011-05-03

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