ES2323911A1 - Method and kit for the detection and identification of estirpes of escherichia coli diarreagenicos. (Machine-translation by Google Translate, not legally binding) - Google Patents

Method and kit for the detection and identification of estirpes of escherichia coli diarreagenicos. (Machine-translation by Google Translate, not legally binding) Download PDF

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Publication number
ES2323911A1
ES2323911A1 ES200503220A ES200503220A ES2323911A1 ES 2323911 A1 ES2323911 A1 ES 2323911A1 ES 200503220 A ES200503220 A ES 200503220A ES 200503220 A ES200503220 A ES 200503220A ES 2323911 A1 ES2323911 A1 ES 2323911A1
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ES
Spain
Prior art keywords
coli
identification
strains
kit
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200503220A
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Spanish (es)
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ES2323911B1 (en
Inventor
Garcia Victor Parro
Garcia Patricia Garrido
Paz Mercedes Moreno
Llorente Carlos Briones
Alvarez Jorge Blanco
Alvarez Miguel Blanco
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidade de Santiago de Compostela
Instituto Nacional de Tecnica Aeroespacial Esteban Terradas
Original Assignee
Universidade de Santiago de Compostela
Instituto Nacional de Tecnica Aeroespacial Esteban Terradas
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Publication date
Application filed by Universidade de Santiago de Compostela, Instituto Nacional de Tecnica Aeroespacial Esteban Terradas filed Critical Universidade de Santiago de Compostela
Priority to ES200503220A priority Critical patent/ES2323911B1/en
Publication of ES2323911A1 publication Critical patent/ES2323911A1/en
Application granted granted Critical
Publication of ES2323911B1 publication Critical patent/ES2323911B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

Method and kit for the detection and identification of diarrheal escherichia coli strains. The present invention consists of a method and a kit for the detection and identification and genotyping of pathogenic strains of escherichia coli causing diarrhea (diarrhea e. Coli), and in particular verotoxin-producing e. Coli (verotoxigenic e. Coli). The body of the invention consists of a group of molecular probes for the identification of strains of verotoxigenic e. Coli and other diarreagenic e. Coli strains. These probes correspond to specific sequences of genetic variants of one or more genes of the lee pathogenicity island. The invention provides specific probes for at least 25 variants of the intimin gene (eae), 4 of the tir gene, 4 of the espa gene, 3 of espb and 3 of espd. These probes are placed in the form of an ordered array (microarray) on solid surfaces (biochip), or they are supplied to perform specific amplifications by pcr. The invention provides control dna for each of the strains. (Machine-translation by Google Translate, not legally binding)
ES200503220A 2005-12-28 2005-12-28 METHOD AND KIT FOR THE DETECTION AND IDENTIFICATION OF ESCHERICHIA COLI DIARREAGENIC STIRPES. Expired - Fee Related ES2323911B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200503220A ES2323911B1 (en) 2005-12-28 2005-12-28 METHOD AND KIT FOR THE DETECTION AND IDENTIFICATION OF ESCHERICHIA COLI DIARREAGENIC STIRPES.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200503220A ES2323911B1 (en) 2005-12-28 2005-12-28 METHOD AND KIT FOR THE DETECTION AND IDENTIFICATION OF ESCHERICHIA COLI DIARREAGENIC STIRPES.

Publications (2)

Publication Number Publication Date
ES2323911A1 true ES2323911A1 (en) 2009-07-27
ES2323911B1 ES2323911B1 (en) 2010-05-14

Family

ID=40852614

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200503220A Expired - Fee Related ES2323911B1 (en) 2005-12-28 2005-12-28 METHOD AND KIT FOR THE DETECTION AND IDENTIFICATION OF ESCHERICHIA COLI DIARREAGENIC STIRPES.

Country Status (1)

Country Link
ES (1) ES2323911B1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005005659A1 (en) * 2003-07-14 2005-01-20 Statens Serum Institut Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp.

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BEKAL, S. et al., "Rapid identification of Escherichia coli pathotypes by virulence gene detection with DNA microarrays.", JOURNAL OF CLINICAL MICROBIOLOGY, 2003, Vol. 41, No. 5, páginas 2113-2125, todo el documento. *
BLANCO, J.E. et al., "Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from human patients: prevalence in Lugo, Spain, from 1992 through 1999.", JOURNAL OF CLINICAL MICROBIOLOGY, 2004, Vol. 42, No. 1, páginas 311-319, todo el documento. *
CHINA, B. et al., "Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction.", FEMS MICROBIOLOGY LETTERS, 1999, Vol. 178, No. 1, páginas 177-182, Materiales y Métodos; Resultados. *
DOBRINDT, U. et al., "Analysis of genome plasticity in pathogenic and commensal Escherichia coli isolates by use of DNA arrays.", JOURNAL OF BACTERIOLOGY, 2003, Vol.185, No. 6, páginas 1831-1840, todo el documento. *
GOFFAUX, F. et al., "Organisation and in vitro expression of esp genes of the LEE (locus of enterocyte effacement) of bovine enteropathogenic and enterohemorrhagic Escherichia coli.", VETERINARY MICROBIOLOGY, 2001, Vol.83, No.3, páginas 275-286, todo el documento. *
JIN, H-Y. et al., "Microarray analysis of Escherichia coli O157:H7.", WORLD JOURNAL OF GASTROENTEROLOGY, 2005 Oct, Vol. 11, No. 37, páginas 5811-5815, todo el documento. *

Also Published As

Publication number Publication date
ES2323911B1 (en) 2010-05-14

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