ES2323217T3 - Metodo para la deteccion fotometrica de iones utilizados en sondas de electroforesis capilar, procedimiento de fabricacion y utilizacion de estas sondas. - Google Patents
Metodo para la deteccion fotometrica de iones utilizados en sondas de electroforesis capilar, procedimiento de fabricacion y utilizacion de estas sondas. Download PDFInfo
- Publication number
- ES2323217T3 ES2323217T3 ES00980902T ES00980902T ES2323217T3 ES 2323217 T3 ES2323217 T3 ES 2323217T3 ES 00980902 T ES00980902 T ES 00980902T ES 00980902 T ES00980902 T ES 00980902T ES 2323217 T3 ES2323217 T3 ES 2323217T3
- Authority
- ES
- Spain
- Prior art keywords
- capillary
- carboxylic acid
- vinyl carboxylic
- capillary electrophoresis
- ions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000523 sample Substances 0.000 title claims abstract description 125
- 238000005251 capillar electrophoresis Methods 0.000 title claims abstract description 58
- 238000001514 detection method Methods 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims description 42
- 238000004519 manufacturing process Methods 0.000 title description 3
- 150000002500 ions Chemical class 0.000 claims abstract description 51
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims abstract description 43
- 150000001875 compounds Chemical class 0.000 claims abstract description 39
- -1 vinyl carboxylic acid compound Chemical class 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000002835 absorbance Methods 0.000 claims abstract description 23
- 230000021615 conjugation Effects 0.000 claims abstract description 19
- 239000006172 buffering agent Substances 0.000 claims abstract description 13
- 238000006073 displacement reaction Methods 0.000 claims abstract description 11
- 239000011203 carbon fibre reinforced carbon Substances 0.000 claims abstract description 10
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims abstract description 10
- 125000000129 anionic group Chemical group 0.000 claims abstract description 9
- 125000002091 cationic group Chemical group 0.000 claims abstract description 9
- 239000008151 electrolyte solution Substances 0.000 claims abstract description 8
- CREMABGTGYGIQB-UHFFFAOYSA-N carbon carbon Chemical group C.C CREMABGTGYGIQB-UHFFFAOYSA-N 0.000 claims abstract description 7
- 230000007423 decrease Effects 0.000 claims abstract description 5
- 230000005684 electric field Effects 0.000 claims abstract description 5
- 238000010521 absorption reaction Methods 0.000 claims description 29
- 238000005370 electroosmosis Methods 0.000 claims description 18
- QFSYADJLNBHAKO-UHFFFAOYSA-N 2,5-dihydroxy-1,4-benzoquinone Chemical compound OC1=CC(=O)C(O)=CC1=O QFSYADJLNBHAKO-UHFFFAOYSA-N 0.000 claims description 16
- PWEBUXCTKOWPCW-UHFFFAOYSA-N squaric acid Chemical compound OC1=C(O)C(=O)C1=O PWEBUXCTKOWPCW-UHFFFAOYSA-N 0.000 claims description 15
- 239000003607 modifier Substances 0.000 claims description 14
- 150000001450 anions Chemical class 0.000 claims description 11
- 239000003792 electrolyte Substances 0.000 claims description 11
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 9
- 125000003118 aryl group Chemical group 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 150000002085 enols Chemical group 0.000 claims description 6
- CSFWPUWCSPOLJW-UHFFFAOYSA-N lawsone Chemical compound C1=CC=C2C(=O)C(O)=CC(=O)C2=C1 CSFWPUWCSPOLJW-UHFFFAOYSA-N 0.000 claims description 6
- 239000007983 Tris buffer Substances 0.000 claims description 5
- 150000007513 acids Chemical class 0.000 claims description 5
- 238000001962 electrophoresis Methods 0.000 claims description 5
- 125000000524 functional group Chemical group 0.000 claims description 5
- 229920002554 vinyl polymer Polymers 0.000 claims description 5
- 150000001412 amines Chemical class 0.000 claims description 4
- 150000001768 cations Chemical class 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 230000002441 reversible effect Effects 0.000 claims description 4
- MDYOLVRUBBJPFM-UHFFFAOYSA-N tropolone Chemical compound OC1=CC=CC=CC1=O MDYOLVRUBBJPFM-UHFFFAOYSA-N 0.000 claims description 4
- FNSQPQKPPGALFA-UHFFFAOYSA-N 6-hydroxy-3,4-dihydro-2h-naphthalen-1-one Chemical compound O=C1CCCC2=CC(O)=CC=C21 FNSQPQKPPGALFA-UHFFFAOYSA-N 0.000 claims description 3
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 claims description 3
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 claims description 3
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 claims description 3
- RBSLJAJQOVYTRQ-UHFFFAOYSA-N croconic acid Chemical compound OC1=C(O)C(=O)C(=O)C1=O RBSLJAJQOVYTRQ-UHFFFAOYSA-N 0.000 claims description 3
- SPXGBDTUWODGLI-UHFFFAOYSA-N deltic acid Chemical compound OC1=C(O)C1=O SPXGBDTUWODGLI-UHFFFAOYSA-N 0.000 claims description 3
- XRWMGCFJVKDVMD-UHFFFAOYSA-M didodecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCC XRWMGCFJVKDVMD-UHFFFAOYSA-M 0.000 claims description 3
- BADXJIPKFRBFOT-UHFFFAOYSA-N dimedone Chemical compound CC1(C)CC(=O)CC(=O)C1 BADXJIPKFRBFOT-UHFFFAOYSA-N 0.000 claims description 3
- JCGNDDUYTRNOFT-UHFFFAOYSA-N oxolane-2,4-dione Chemical compound O=C1COC(=O)C1 JCGNDDUYTRNOFT-UHFFFAOYSA-N 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 229940116269 uric acid Drugs 0.000 claims description 3
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims description 3
- 150000007530 organic bases Chemical class 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- RLGQACBPNDBWTB-UHFFFAOYSA-N cetyltrimethylammonium ion Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)C RLGQACBPNDBWTB-UHFFFAOYSA-N 0.000 claims 2
- GXHFUVWIGNLZSC-UHFFFAOYSA-N meldrum's acid Chemical compound CC1(C)OC(=O)CC(=O)O1 GXHFUVWIGNLZSC-UHFFFAOYSA-N 0.000 claims 2
- KZNICNPSHKQLFF-UHFFFAOYSA-N succinimide Chemical compound O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 claims 2
- 150000001649 bromium compounds Chemical group 0.000 claims 1
- VGMVMTKGFSFRLO-UHFFFAOYSA-N cyclopentene-1,2-diol Chemical compound OC1=C(O)CCC1 VGMVMTKGFSFRLO-UHFFFAOYSA-N 0.000 claims 1
- 125000002587 enol group Chemical group 0.000 abstract 1
- CYIDZMCFTVVTJO-UHFFFAOYSA-N pyromellitic acid Chemical compound OC(=O)C1=CC(C(O)=O)=C(C(O)=O)C=C1C(O)=O CYIDZMCFTVVTJO-UHFFFAOYSA-N 0.000 description 28
- 230000037230 mobility Effects 0.000 description 22
- 239000012491 analyte Substances 0.000 description 18
- 239000002253 acid Substances 0.000 description 10
- 238000002347 injection Methods 0.000 description 7
- 239000007924 injection Substances 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000004255 ion exchange chromatography Methods 0.000 description 6
- ZCDOYSPFYFSLEW-UHFFFAOYSA-N chromate(2-) Chemical compound [O-][Cr]([O-])(=O)=O ZCDOYSPFYFSLEW-UHFFFAOYSA-N 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 229910021642 ultra pure water Inorganic materials 0.000 description 4
- 239000012498 ultrapure water Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000002745 absorbent Effects 0.000 description 3
- 239000002250 absorbent Substances 0.000 description 3
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 239000004065 semiconductor Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000002211 ultraviolet spectrum Methods 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- 102000015636 Oligopeptides Human genes 0.000 description 2
- 108010038807 Oligopeptides Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 150000001408 amides Chemical class 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 150000001449 anionic compounds Chemical class 0.000 description 2
- 150000001491 aromatic compounds Chemical class 0.000 description 2
- 235000019445 benzyl alcohol Nutrition 0.000 description 2
- 150000001735 carboxylic acids Chemical class 0.000 description 2
- 150000001767 cationic compounds Chemical class 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000005350 fused silica glass Substances 0.000 description 2
- 229910001412 inorganic anion Inorganic materials 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 150000002892 organic cations Chemical group 0.000 description 2
- 125000005372 silanol group Chemical group 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 238000003419 tautomerization reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- RPLSBADGISFNSI-UHFFFAOYSA-N 2,2-dimethyl-1,3-dioxane Chemical compound CC1(C)OCCCO1 RPLSBADGISFNSI-UHFFFAOYSA-N 0.000 description 1
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000005515 capillary zone electrophoresis Methods 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 150000001923 cyclic compounds Chemical class 0.000 description 1
- BQXFQDOHKMTBDK-UHFFFAOYSA-N cysteinyl radical Chemical compound [S]CC(N)C(O)=O BQXFQDOHKMTBDK-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000001072 heteroaryl group Chemical group 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical compound [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 150000002891 organic anions Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 125000001453 quaternary ammonium group Chemical group 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- WCJLIWFWHPOTAC-UHFFFAOYSA-N rhodizonic acid Chemical compound OC1=C(O)C(=O)C(=O)C(=O)C1=O WCJLIWFWHPOTAC-UHFFFAOYSA-N 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- PWEBUXCTKOWPCW-UHFFFAOYSA-L squarate Chemical compound [O-]C1=C([O-])C(=O)C1=O PWEBUXCTKOWPCW-UHFFFAOYSA-L 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000033772 system development Effects 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
- PBYZMCDFOULPGH-UHFFFAOYSA-N tungstate Chemical compound [O-][W]([O-])(=O)=O PBYZMCDFOULPGH-UHFFFAOYSA-N 0.000 description 1
- 238000004457 water analysis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Electrostatic Separation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16842799P | 1999-11-30 | 1999-11-30 | |
| US168427P | 1999-11-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2323217T3 true ES2323217T3 (es) | 2009-07-09 |
Family
ID=22611441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00980902T Expired - Lifetime ES2323217T3 (es) | 1999-11-30 | 2000-11-30 | Metodo para la deteccion fotometrica de iones utilizados en sondas de electroforesis capilar, procedimiento de fabricacion y utilizacion de estas sondas. |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US6953520B2 (enExample) |
| EP (1) | EP1257812B1 (enExample) |
| JP (2) | JP3807984B2 (enExample) |
| KR (1) | KR100564085B1 (enExample) |
| AT (1) | ATE427482T1 (enExample) |
| AU (1) | AU1810101A (enExample) |
| CA (1) | CA2394847C (enExample) |
| DE (1) | DE60041928D1 (enExample) |
| ES (1) | ES2323217T3 (enExample) |
| WO (1) | WO2001040785A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2394847C (en) * | 1999-11-30 | 2007-01-16 | Electric Power Research Institute | Capillary electrophoresis probes and method |
| US9658178B2 (en) | 2012-09-28 | 2017-05-23 | General Electric Company | Sensor systems for measuring an interface level in a multi-phase fluid composition |
| US9538657B2 (en) | 2012-06-29 | 2017-01-03 | General Electric Company | Resonant sensor and an associated sensing method |
| US9536122B2 (en) | 2014-11-04 | 2017-01-03 | General Electric Company | Disposable multivariable sensing devices having radio frequency based sensors |
| US7691329B2 (en) * | 2006-11-16 | 2010-04-06 | General Electric Company | Methods for detecting contaminants in a liquid |
| US9589686B2 (en) | 2006-11-16 | 2017-03-07 | General Electric Company | Apparatus for detecting contaminants in a liquid and a system for use thereof |
| US10914698B2 (en) | 2006-11-16 | 2021-02-09 | General Electric Company | Sensing method and system |
| JP5158747B2 (ja) * | 2007-01-12 | 2013-03-06 | 独立行政法人産業技術総合研究所 | 除血球機能を統合した電気泳動チップを用いた一酸化窒素最終代謝物の分析法 |
| US8542023B2 (en) | 2010-11-09 | 2013-09-24 | General Electric Company | Highly selective chemical and biological sensors |
| EA030927B1 (ru) | 2012-08-22 | 2018-10-31 | Дженерал Электрик Компани | Система и способ беспроводного измерения параметров рабочего состояния машины |
| US10598650B2 (en) | 2012-08-22 | 2020-03-24 | General Electric Company | System and method for measuring an operative condition of a machine |
| US10684268B2 (en) | 2012-09-28 | 2020-06-16 | Bl Technologies, Inc. | Sensor systems for measuring an interface level in a multi-phase fluid composition |
| RU2499989C1 (ru) * | 2012-11-14 | 2013-11-27 | Государственное научное учреждение Северо-Кавказский зональный научно-исследовательский институт садоводства и виноградарства Россельхозакадемии | Способ определения общего фосфора методом капиллярного электрофореза |
| RU2554799C2 (ru) * | 2013-11-06 | 2015-06-27 | Федеральное государственное бюджетное научное учреждение "Северо-Кавказский зональный научно-исследовательский институт садоводства и виноградарства" | Способ определения общего азота методом капиллярного электрофореза |
| US12203130B2 (en) | 2018-12-20 | 2025-01-21 | Krylov Sergey N | Binder selection using capillary electrophoresis |
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| DE2623836C3 (de) * | 1976-05-28 | 1978-12-21 | Chemische Werke Huels Ag, 4370 Marl | Verfahren zur Herstellung von Quadratsäure |
| US4104308A (en) | 1976-06-21 | 1978-08-01 | International Business Machines Corporation | Synthesis of squaric acid |
| DE2850078A1 (de) | 1978-11-18 | 1980-05-29 | Basf Ag | Aminopropanolderivate des 6-hydroxy-2,3,4,5-tetrahydro-1h-1- benzazepin-2-ons |
| US4589964A (en) * | 1980-10-08 | 1986-05-20 | American Hospital Supply Corporation | Process for graft copolymerization of a pre-formed substrate |
| US4478694A (en) | 1983-10-11 | 1984-10-23 | Ska Associates | Methods for the electrosynthesis of polyols |
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| US4680201A (en) * | 1985-10-30 | 1987-07-14 | Stellan Hjerten | Coating for electrophoresis tube |
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| US4828983A (en) * | 1986-07-10 | 1989-05-09 | Eastman Kodak Company | Use of phenols and anilines to increase the rate of peroxidase catalyzed oxidation of leuco dyes |
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| US5215890A (en) | 1988-11-03 | 1993-06-01 | Spyros Theodoropulos | Chromogenic substrate to peroxidase enzymes |
| US5061361A (en) * | 1989-03-06 | 1991-10-29 | Hewlett-Packard Company | Capillary zone electrophoresis cell system |
| DE69006091T2 (de) * | 1989-04-14 | 1994-05-05 | Kontron Instr Holding | Kapillare Durchflusszelle. |
| US5104506A (en) | 1990-01-29 | 1992-04-14 | Millipore Corporation | Method for separating ionic species using capillary electrophoresis |
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| JP3170891B2 (ja) | 1992-08-31 | 2001-05-28 | 株式会社島津製作所 | 間接吸光度検出法によるキャピラリ電気泳動分析方法 |
| US5288811A (en) | 1992-11-05 | 1994-02-22 | Exxon Research And Engineering Company | Cyclic carbonyl containing compounds via radical grafting |
| US5866683A (en) | 1995-03-31 | 1999-02-02 | Laboratory Of Molecular Biophotonics | Isoelectric point markers for isoelectric focusing with fluorescence detection |
| DE69706417T2 (de) * | 1996-04-19 | 2002-06-27 | Amersham Pharmacia Biotech Uk Ltd., Little Chalfont | Quadratfarbstoffe und deren verwendung in einem fluorescenzsequenzierungsverfahren |
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-
2000
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- 2000-11-30 KR KR1020027006873A patent/KR100564085B1/ko not_active Expired - Fee Related
- 2000-11-30 DE DE60041928T patent/DE60041928D1/de not_active Expired - Lifetime
- 2000-11-30 ES ES00980902T patent/ES2323217T3/es not_active Expired - Lifetime
- 2000-11-30 AU AU18101/01A patent/AU1810101A/en not_active Abandoned
- 2000-11-30 AT AT00980902T patent/ATE427482T1/de not_active IP Right Cessation
- 2000-11-30 US US09/728,723 patent/US6953520B2/en not_active Expired - Lifetime
- 2000-11-30 EP EP00980902A patent/EP1257812B1/en not_active Expired - Lifetime
- 2000-11-30 WO PCT/US2000/032688 patent/WO2001040785A1/en not_active Ceased
- 2000-11-30 JP JP2001542199A patent/JP3807984B2/ja not_active Expired - Fee Related
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2005
- 2005-04-04 JP JP2005107863A patent/JP3808085B2/ja not_active Expired - Fee Related
- 2005-09-21 US US11/233,353 patent/US20060065530A1/en not_active Abandoned
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| US20060065530A1 (en) | 2006-03-30 |
| KR20020077348A (ko) | 2002-10-11 |
| JP3808085B2 (ja) | 2006-08-09 |
| WO2001040785A1 (en) | 2001-06-07 |
| EP1257812B1 (en) | 2009-04-01 |
| US20020092768A1 (en) | 2002-07-18 |
| DE60041928D1 (de) | 2009-05-14 |
| US6953520B2 (en) | 2005-10-11 |
| JP2003515742A (ja) | 2003-05-07 |
| JP2005265853A (ja) | 2005-09-29 |
| ATE427482T1 (de) | 2009-04-15 |
| KR100564085B1 (ko) | 2006-03-27 |
| EP1257812A1 (en) | 2002-11-20 |
| JP3807984B2 (ja) | 2006-08-09 |
| CA2394847C (en) | 2007-01-16 |
| AU1810101A (en) | 2001-06-12 |
| EP1257812A4 (en) | 2006-06-07 |
| CA2394847A1 (en) | 2001-06-07 |
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