ES2263990T3 - ORALLY ADMINISTRABLE COMPOSITION FOR THE PHOTOPROTECTION OF THE SKIN. - Google Patents

Abstract

Orally administrable composition for skin photoprotection which comprises i) at least one bacterium of a probiotic lactic acid or a culture supernatant thereof and ii) at least one carotenoid, included in an orally acceptable carrier, said composition additionally comprising a yeast extract.

Description

Orally administrable composition for skin photoprotection.

Field of the Invention

The present invention relates to a composition or pharmaceutical compositions orally administrable, or cosmetic compositions, for the protection of skin against the negative effects of the environment, in particular the exposure to solar radiation, which is orally administrable and its use to improve skin photoprotection.

Background of the invention

The continuous reduction of the ozone layer of the atmosphere with the concurrent increase in ultraviolet radiation which reaches the surface of the planet has attracted a lot of interest in its possible consequences on human health. Although the exposure to ultraviolet radiation is necessary for beings humans to produce vitamin D, increasing evidence suggests that extensive exposure to sunlight, particularly radiation ultraviolet, causes a variety of skin problems, including the induction of certain skin cancers and the induction of a accelerated skin aging.

In addition to these established concerns on health, research has also provided evidence suggesting that exposure to ultraviolet radiation may negatively affect a variety of immunity responses in humans both locally, within the skin irradiated with ultraviolet rays, as well as systematically, that is in distant places of the irradiated skin.

It is therefore important to relieve the effects harmful of ultraviolet radiation on the skin and also avoid the development of erythema, edema and peeling or peeling (hyperkeratosis) of the skin.

In the art, there are various attempts, such such as the use of solar screens or other agents Particular pharmacological

In the journal Dermatological Research, 97 (1991), 624-628 it is reported that the topical application of compounds that absorb ultraviolet radiation (screens solar) is effective in preventing erythema and induced edema by ultraviolet radiation but can't prevent suppression immunological induced by ultraviolet light. This finding is confirmed by various other studies according to which sunscreens seem to prevent inflammation or irritation but they do not provide complete prophylactic protection against effects of immunological suppression of radiation ultraviolet.

On the other hand, in document FR 2698 268 (L'Oreal) presents an orally administrable composition that comprises a combination of at least one of an amino acid, a copper salt and a mixture of vitamins to protect the skin against ultraviolet radiation

However, there is still a need in the technique of an orally administrable composition that is capable of improve and strengthen the skin's photoprotective function.

Summary of the Invention

Accordingly, in a first aspect the The present invention is intended to provide a composition orally administrable for skin photoprotection which comprises an effective photoprotective amount of i) at least one bacterium of a probiotic lactic acid and a yeast extract or a culture supernatant thereof and ii) at least one carotenoid or either a derivative, included in an orally acceptable carrier.

The present invention further relates to the use of an effective photoprotective amount of at less a bacterium of a probiotic lactic acid or a culture supernatant thereof and at least one carotenoid, included in an orally acceptable carrier to prepare a composition orally administrable to protect the skin against radiation solar such as ultraviolet and all disorders related skin, such as erythema, inflammation, sunburn, barrier function, photoaging, impaired immune system, for example.

In a final aspect, the invention relates to oral administration to an individual of a composition that comprises an effective photoprotective amount of i) at least one bacterium of a probiotic lactic acid or a supernatant culture of the same and ii) at least one carotenoid or a derivative, in a orally acceptable carrier and its use to improve photoprotective function of the skin.

The combination in accordance with this invention has a particular beneficial effect in the protection of skin and skin coloring that helps reduce the effects of the tension related to solar radiation on the skin.

Detailed description of the invention

Within the description that follows then the acronym "NCC" designates the Collection of Nestlé Crops (Nestlé Culture Collection) (research center Nestle, Vers-chez-les-Blanc, Lausanne, Switzerland). The term "photoprotection" is used to describe the attempt to block or reduce clinical effects, histological and immunological adverse exposure to solar radiation of the skin.

In accordance with the present invention, the subject compositions comprise, as the active agents of the same, combinatorial mixtures of at least one acid bacteria lactic probiotic or a culture supernatant thereof and so less a carotenoid or a derivative.

Of course, it has now been determined so surprising and unexpected that the mixture of these two constituents very specific causes an improved effect or response with regarding skin photoprotection.

Probiotics are non-pathogenic organisms and non-toxicogens that survive the passage through the stomach and the small intestine. Through continued ingestion by the host they can eventually colonize the intestine to an extent substantial thus competing with other bacteria potentially pathogenic for nutrients and places of fixation in the gastrointestinal wall and reducing their numbers and reducing or avoiding infections. So far they have been found a series of different probiotic microorganisms, on all which are reported to exert their effects on the intestine through of the production of toxins, metabolic by-products, acids short chain fatty acids and the like.

It has now been shown that probiotics exercise also an effect on the individual's body in a location distant from the area in which they colonize. And particularly, surprisingly it has been found that you can get a composition provided with a synergistic photoprotective effect on the skin combining in an orally acceptable carrier a microorganism probiotic and an active compound such as for example a carotenoid

In a preferred embodiment, the probiotic to be included in the carrier is selected from the group containing lactic acid bacteria, in particular Lactobacilli and Bifidobacteria and more preferably Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus casecase, Lactobacillus casecase or Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium animalis, Bifidobacterium lactis, Bifidobacterium infantis, Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum or a mixture thereof.

According to a more preferred embodiment, the Lactobacillus johnsonii NCC 533, Lactobacillus paracasei NCC 2461, Bifidobacterium adolescentis NCC 251 and Bifidobacterium longum NCC 490 strains were deposited by way of example, under the Budapest treaty at the Pasteur Institute (Street of Doctor Roux, 28, F-75024, Paris, code 15) on 30.06.92, 12.01.99, 15.04.99 and 15.03.99 respectively and under the deposit numbers CNCM I-1225, CNCM I-2116, CNCM I- 2168 and CNCM I-2170, respectively.

The strain Bifidobacterium lactis (ATCC27536) provided by Hansen can also be used (Chr. Hansen A / S, 10-12 Boege Alle, PO Box 407, DK-2970 Hoersholm, Denmark).

The probiotic microorganism according to the The present invention can be included in live form, in form dead, semi-activated or deactivated and fragments or fractions that originate from the microorganism both live or dead, for example as a lyophilized powder. They can also be included in the products supernatant cultures of the microorganisms, optionally in concentrated form. They can also be included in encapsulated form. When a culture supernatant is used probiotic the supernatant can be used as such or it can be subjected to one or more purification steps prior to inclusion in the product, to concentrate or isolate the ingredient or metabolite active. The procedure and techniques for purification of compounds and the detection of their activity in the Obtained fractions are well known by experts in the matter.

Probiotic lactic acid bacteria can be present in the carrier in an amount of at least 10 5 cfu / g carrier and preferably from 10 5 to 10 15 cfu / g orally acceptable carrier and more preferably from 10 7 to 10 12 cfu / g of carrier orally acceptable.

The carotenoid may be a carotenoid with or without provitamin A activity. It may be? -Carotene,? -Carotene,? -Carotene, lycopene, zeaxanthin, lutein or a mixture thereof. The carotenoid can be obtained from a synthetic or natural origin or be contained in a natural extract. When the carotenoid is obtained from a natural origin, it is preferably obtained from plant material, in which the plant is grown live or in vitro . The procedure for the extraction of carotenoids is well known in the art. The carotenoid may be present in the carrier in an amount of from 10-12% to 20% by weight and preferably from 0.00001 mg to 50 mg / day and more preferably from 0.001 mg to 30 mg / day.

You can also use a mixture of one plurality of lactic acid bacteria or carotenoids.

The carrier can be any product food or pharmaceutical, or a nutritional supplement for oral administration or a composition for oral administration, in which the probiotic microorganism and the carotenoid may be included Examples of food or pharmaceutical carriers are milk, yogurt, curd, cheese, fermented milks, products fermented milk products, ice cream, products derived from fermented cereals, milk derived powders, formulas or tablets for infants, liquid suspensions, oral supplements dry, wet oral supplements, dry tube feeding. He Nutritional supplement for oral administration can be in capsule form, soft capsules, tablets, pills or pills, gums, or drinkable solutions or emulsions. The procedures for Carrier preparation are common knowledge.

The composition according to the invention may additionally comprise bioactive yeast molecules or extracts, for example. In a preferred embodiment, the yeast is a food grade yeast selected from the group containing Ascomycotin or Deuteromycotin. In a preferred embodiment, the yeast can be selected from the group containing Debaryomyces, Kluyveromyces, Saccharomyces, Yarrowia, Zygosaccharomyces, Candida and Rhodutorula and more preferably Saccharomyces caerevisae (baker's yeast).

A yeast of this type can be used in form of dried or lyophilized extracts. It can be present in the carrier in an amount of at least about 10 5 orally acceptable carrier cfu / g, preferably from 10 5 to 10 15 cfu / g of orally acceptable carrier and more preferably from 10 7 to 10 12 cfu / g of carrier orally acceptable, such amounts depending on the nature and the activity of the particular yeast.

The composition according to the invention it can also comprise normal excipients, in particular sweeteners, aromatic agents or preservatives.

The composition according to the invention provides an amazing protective and preventive effect of the skin and synergistic

Accordingly, in another aspect, the invention refers to oral administration to an individual of a composition comprising an effective photoprotective amount of i) at least one bacterium of a probiotic lactic acid or a culture supernatant thereof and ii) at least one carotenoid or either a derivative, in an orally acceptable carrier and used to improve the skin's photoprotective function.

The amount of composition that will be consumed by the individual will depend on the desirable effect. Without However, a quantity of composition will generally be suitable for provide a daily amount of about 10 5 to 10 15 organisms, organisms which may be alive or dead and from 0.00001 mg to 50 mg of carotenoids.

The composition is administered to an individual before or during exposure to ultraviolet radiation, in particular sun exposure. When the exposure period is foreseeable, it is desirable to start consumption before exposure and preferably 1 to 2 months before and prolong consumption for The exhibition.

The following examples are provided by title of illustration. All percentages are given by weight to Unless indicated otherwise.

Examples

In the following examples 1 to 6, \ beta-carotene is provided by Roche, Lycopene is provided by Lycored, S. cerevissae lyophilisate is provided by BioSpringer, a dry mixture of Lactobacillus CNCM I-1225, a dry mixture of Lactobacillus CNCM prepare I-2116 or Bifidobacterium CNCM I-2168 so that they contain 1.10 8 to 1.10 9 organisms.

Example 1

A photoprotective composition orally Daily administrable is prepared as follows:

β-carotene 4.7 mg A dry mixture of Lactobacillus CNCM I-1225 50 mg Glucidex IT 19 (maltodextrin powder) QSP 500 mg

The composition is administered to the individual in a 2 x 500 mg daily, which provides an effect Protective and preventive skin.

Example 2

A photoprotective composition orally Daily administrable is prepared as follows:

β-carotene 4.7 mg Zeaxanthin 10 mg A dry mix of Lactobacillus CNCM I-1225 fifty mg Glucidex IT 19 (powder maltodextrin) QSP 500 mg

The composition is administered to the individual in a 2 x 500 mg daily, which provides an effect Protective and preventive skin.

Example 3

A photoprotective composition orally Daily administrable is prepared as follows:

β-carotene 4.7 mg Lycopene 2.5 mg A dry mix of Bifidobacterium CNCM I-2168 30 mg A mix lactobacillus CNCM I-1225 30 mg Glucidex IT 19 (powder maltodextrin) QSP 500 mg

The composition is administered to the individual in a 2 x 500 mg daily, which provides an effect Protective and preventive skin.

Example 4

A photoprotective composition orally Daily administrable is prepared as follows:

Lycopene 2.5 mg Lyophilized S. cerevissae 75 mg A dry mixture of Lactobacillus CNCM I-2116 50 mg Glucidex IT 19 (maltodextrin powder) QSP 500 mg

The composition is administered to the individual in a 2 x 500 mg daily, which provides an effect Protective and preventive skin.

Example 5

A photoprotective composition orally Daily administrable is prepared as follows:

β-carotene 4.7 mg Lycopene 2.5 mg Lyophilized S. cerevissae 75 mg A mix lactobacillus CNCM I-1225 fifty mg Glucidex IT 19 (powder maltodextrin) QSP 500 mg

The composition is administered to the individual in a 2 x 500 mg daily, which provides an effect Protective and preventive skin.

Example 6

A photoprotective composition orally Daily administrable is prepared as follows:

β-carotene 4.7 mg Lyophilized S. cerevissae 75 mg A dry mixture of Lactobacillus CNCM I-1225 50 mg Glucidex IT 19 (maltodextrin powder) QSP 500 mg

The composition is administered to the individual in a 2 x 500 mg daily, which provides an effect Protective and preventive skin.

Claims (14)

1. Composition orally administrable for skin photoprotection which comprises i) at least one bacterium of a probiotic lactic acid or a supernatant culture of the same and ii) at least one carotenoid, included in a carrier orally acceptable, said composition further comprising a yeast extract. 2. Composition according to claim 1 in which lactic acid bacteria is selected from the group comprising lactic acid bacteria, preferably Lactobacilli and Bifidobacteria. 3. Composition according to claim 1 or 2 in which the lactic acid bacterium is Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus casei or Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium bumidobacterium bumidobacterium bumidobacterium Bifidobacterium infantis, Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum or a mixture thereof. 4. Composition according to any of the claims 1 to 3 in which the lactic acid bacterium is CNCM I-1225, CNCM I-2116, CNCM I-2168 and CNCM I-2170. 5. Composition according to any of the claims 1 to 4 in which the lactic acid bacterium is included in the carrier lively, semi-actively or in form deactivated, preferably as a lyophilized powder. 6. Composition according to any of the claims 1 to 5 in which the carotenoid is a carotenoid with or without provitamin A activity, such as β-carotene, γ-carotene, α-carotene, lycopene, zeaxanthin, lutein or A mixture of them. 7. Composition according to any of the claims 1 to 6 in which the carrier can be any food or pharmaceutical product, or a nutritional supplement For oral administration. 8. Composition according to claim 6 in which the food or pharmaceutical carriers are milk, yogurt, curd, cheese, fermented milks, fermented products milk products, ice cream, cereal products fermented, milk derived powders, formulas or tablets for infants, liquid suspensions, dry oral supplements, Wet oral supplements, dry tube feeding. 9. Composition according to claim 7 in which the nutritional supplement for oral administration It can be in the form of capsules, soft capsules, tablets, pills or pills, gums, or drinkable solutions or emulsions. 10. Use of at least one bacterium from probiotic lactic acid or a culture supernatant thereof and by at least one carotenoid, included in a carrier orally acceptable, for the preparation of a composition orally administrable for the protection of the skin against radiation solar and attenuation or prevention of all disorders related to the skin. 11. Use according to claim 10 wherein the lactic acid bacterium is Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus casei or Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium bifidobacterium bifidobacterium bifidobacterium bifidobacterium infantis, Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum or a mixture thereof. 12. Use according to claim 10 or 11 in which the lactic acid bacterium is CNCM I-1225, CNCM I-2116, CNCM I-2168, CNCM I-2170 or ATCC 27536. 13. Use in accordance with any of the claims 10 to 12 wherein the lactic acid bacteria probiotic is present in the carrier in an amount of from 10 5 to 10 12 cfu / g carrier. 14. Use in accordance with any of the claims 10 to 12 in which the carotenoid is present in the carrier in an amount of from 10-12% to 20% in weight.