ES2237316B1 - KIT AND PROCEDURE TO IDENTIFY POLLUTION BY AERUGINOS PSEUDOMONAS OF SANITARY PRODUCTS. - Google Patents
KIT AND PROCEDURE TO IDENTIFY POLLUTION BY AERUGINOS PSEUDOMONAS OF SANITARY PRODUCTS.Info
- Publication number
- ES2237316B1 ES2237316B1 ES200302640A ES200302640A ES2237316B1 ES 2237316 B1 ES2237316 B1 ES 2237316B1 ES 200302640 A ES200302640 A ES 200302640A ES 200302640 A ES200302640 A ES 200302640A ES 2237316 B1 ES2237316 B1 ES 2237316B1
- Authority
- ES
- Spain
- Prior art keywords
- pseudomonas aeruginosa
- contamination
- medical devices
- procedure
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 13
- 241000589516 Pseudomonas Species 0.000 title description 3
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 22
- 238000011109 contamination Methods 0.000 claims abstract description 14
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000004599 antimicrobial Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 230000002401 inhibitory effect Effects 0.000 claims description 4
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 229960002798 cetrimide Drugs 0.000 claims description 3
- 239000001982 cetrimide agar Substances 0.000 claims 1
- 238000009630 liquid culture Methods 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 7
- 230000000845 anti-microbial effect Effects 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000006152 selective media Substances 0.000 description 3
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 210000004087 cornea Anatomy 0.000 description 2
- 201000007717 corneal ulcer Diseases 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 208000032536 Pseudomonas Infections Diseases 0.000 description 1
- 206010064996 Ulcerative keratitis Diseases 0.000 description 1
- 235000020682 bottled natural mineral water Nutrition 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Kit y procedimiento para identificar la contaminación por pseudomonas aeruginosa de los productos sanitarios. Se ha desarrollado un procedimiento para detectar la contaminación por Pseudomonas aeruginosa en productos sanitarios y prevenir infecciones producidas por Pseudomonas aeruginosa. Utilizando el kit de identificación propuesto puede detectarse la contaminación sin necesidad de enviar las muestras al laboratorio.Kit and procedure to identify contamination by pseudomonas aeruginosa of medical devices. A procedure has been developed to detect Pseudomonas aeruginosa contamination in medical devices and prevent infections caused by Pseudomonas aeruginosa. Using the proposed identification kit, contamination can be detected without sending the samples to the laboratory.
Description
Kit y procedimiento para identificar la contaminación por Pseudomonas aeruginosa de productos sanitarios.Kit and procedure to identify Pseudomonas aeruginosa contamination of medical devices.
Este procedimiento se puede encuadrar dentro de la prevención de infecciones por Pseudomonas aeruginosa.This procedure can be framed within the prevention of Pseudomonas aeruginosa infections.
En la actualidad, para detectar contaminación por Pseudomonas aeruginosa es necesario, a nivel de laboratorio, sembrar la muestra en un medio de cultivo selectivo para dicha bacteria (Szita-G. A novel, Selective Synthetic Acetamide Containing Culture-Medium for Isolating Pseudomonas aeruginosa from Milk. International Journal of Food Microbiology 1998; 43, Iss 1-2:123-127; Legnani P. Survival and Growth of Pseudomonas aeruginosa in Natural Mineral-Water A 5-Year Study. International Journal of Food Microbiology 1999; 53, Iss 2-3:153-158; Jayasekara NY. Populations of Pseudomonass and Related Bacteria Associated with Bottled Noncarbonated Mineral-Water. Food Microbiology 1998; 15, Iss 2:167-'176; Salvat G. A Selective medium for the Rapid Detection by an Impedance Technique of Pseudomonass spp. Associated with Poultry Meat. Journal of Appiled Microbiology 1997; 83, Iss 4:456-463).At present, to detect contamination by Pseudomonas aeruginosa it is necessary, at the laboratory level, to sow the sample in a selective culture medium for said bacterium (Szita-G. A novel, Selective Synthetic Acetamide Containing Culture-Medium for Isolating Pseudomonas aeruginosa from Milk. International Journal of Food Microbiology 1998; 43, Iss 1-2: 123-127; Legnani P. Survival and Growth of Pseudomonas aeruginosa in Natural Mineral-Water A 5-Year Study. International Journal of Food Microbiology 1999; 53, Iss 2-3: 153-158; Jayasekara NY. Populations of Pseudomonass and Related Bacteria Associated with Bottled Noncarbonated Mineral-Water. Food Microbiology 1998; 15, Iss 2: 167 -176; Salvat G. A Selective medium for the Rapid Detection by an Impedance Technique of Pseudomonass spp. Associated with Poultry Meat. Journal of Appiled Microbiology 1997; 83, Iss 4: 456-463).
Cuando la detección de la contaminación se hace en productos sanitarios, la composición en agentes antimicrobianos de dichos productos hace inviable el crecimiento de la bacteria en el medio de cultivo, aunque la bacteria esté presente y viable.When pollution detection is done in medical devices, the composition in antimicrobial agents of these products makes the growth of the bacteria in the culture medium, although the bacteria is present and viable.
Sería necesario inhibir la actividad antimicrobiana de la muestra previamente a la siembra en el medio de cultivo (Holt JG, Krieg NR, Sneath PH, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology. 9ª ed. Baltimore (Maryland): Wiliams & Wilkkins;1994.).It would be necessary to inhibit the activity antimicrobial sample prior to planting in the medium of cultivation (Holt JG, Krieg NR, Sneath PH, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology. 9th ed. Baltimore (Maryland): Wiliams &Wilkkins; 1994.).
La preparación del medio inhibidor de antimicrobianos y del medio selectivo para Pseudomonas aeruginosa hace costosa y lenta la técnica a nivel particular.The preparation of the antimicrobial inhibitor medium and the selective medium for Pseudomonas aeruginosa makes the technique at a particular level expensive and slow.
La producción de un Kit con ambos medios, inhibidor de antimicrobiano y selectivo para la bacteria, para su uso inmediato facilitaría en tiempo y coste económico esta técnica preventiva.The production of a Kit with both media, antimicrobial inhibitor and selective for the bacterium, for its immediate use would facilitate this technique in time and economic cost preventive
Como ejemplo de aplicación concreta, se ha demostrado que la mayoría de las úlceras cornéales infecciosas está asociada al uso de lentes de contacto hidrofilicas o blandas (LCH) (Stern GA, Zam SZ. The pathogenesis of contact lees associated Pseudomonas aeruginosa corneal úlceration. Cornea 1986; 5:41-45; Spurr-Michaud SJ, Barza M, Gipson IK, An organ culture system for study of adherence of Pseudomonas aeruginosa to normal and wounded corneas. Invest Ophthalmol Vis Sci 1988; 29:379), y el agente etiológico principal es Pseudomonas aeruginosa. Sería conveniente para la prevención de esta grave patología que se pudiera controlar la presencia de contaminación por esta bacteria en los sistemas de mantenimiento de las LCH. Esto es posible haciendo un control de contaminación por un profesional sanitario tomando muestras de los productos de mantenimiento.As an example of concrete application, it has been shown that the majority of infectious corneal ulcers is associated with the use of hydrophilic or soft contact lenses (LCH) (Stern GA, Zam SZ. The pathogenesis of contact lees associated Pseudomonas aeruginosa corneal ulcer. Cornea 1986; 5: 41-45; Spurr-Michaud SJ, Barza M, Gipson IK, An organ culture system for study of adherence of Pseudomonas aeruginosa to normal and wounded corneas. Invest Ophthalmol Vis Sci 1988; 29: 379), and the agent main etiologic is Pseudomonas aeruginosa . It would be convenient for the prevention of this serious pathology that the presence of contamination by this bacterium could be controlled in the LCH maintenance systems. This is possible by contamination control by a healthcare professional taking samples of maintenance products.
El procedimiento para la detección de Pseudomonas aeruginosa consiste en tomar muestras con la torunda de algodón estéril, impregnada en el medio inhibidor de antimicrobianos, del producto sanitario a analizar. Tras la toma de muestras se vuelve a introducir la torunda en el tubo de ensayo con el medio inhibidor y de deja) actuar entre 5 y 15 minutos para neutralizar los agentes antimicrobianos del producto sanitario e impedir que estos puedan inhibir el crecimiento de la bacteria si estuviera presente. Una vez transcurrido ese tiempo se saca la torunda del tubo de ensayo y se siembra en la placa de Cetrimida Agar Base. Esta placa de lleva a incubar entre 37ºC y 43ºC durante 48 horas. Si tras ese tiempo hay crecimiento bacteriano en la placa el resultado es positivo para contaminación por pseudomonas aeruginosa en el producto sanitario estudiado.The procedure for the detection of Pseudomonas aeruginosa consists in taking samples with the sterile cotton swab, impregnated in the antimicrobial inhibitor medium, of the medical device to be analyzed. After sampling, the swab is reintroduced into the test tube with the inhibitory medium and allowed to act for 5 to 15 minutes to neutralize the antimicrobial agents of the medical device and prevent them from inhibiting the growth of the bacteria if be present Once this time has elapsed, the swab is removed from the test tube and seeded in the Cetrimide Base Agar plate. This plate is incubated between 37 ° C and 43 ° C for 48 hours. If after that time there is bacterial growth in the plaque the result is positive for contamination by pseudomonas aeruginosa in the studied medical device.
Se trata de unir en un Kit el medio de cultivo inhibidor de los agentes microbianos, la placa Petri con el medio selectivo para Pseudomonas aeruginosa y la torunda de algodón estéril para la toma de muestras.It involves joining in a Kit the culture medium inhibitor of microbial agents, the Petri dish with the selective medium for Pseudomonas aeruginosa and the sterile cotton swab for sampling.
Para la toma muestras se utilizan escobillones estériles que vienen dentro de un tubo de ensayo de plástico también estéril, este tubo de ensayo lleva 3 ml de caldo de Dey Engley. Todo se hace en condiciones de esterilidad. Se sacan los escobillones impregnados en Dey Engley del tubo de ensayo y se toma la muestra mojando el escobillón en la solución de mantenimiento que contiene el estuche y se frotan las paredes del estuche, a continuación se meten de nuevo en el tubo de ensayo que contiene 3 ml de caldo de Dey Engley estéril y se deja actuar durante diez minutos para neutralizar los restos de los agentes antimicrobianos de las soluciones desinfectantes de mantenimiento e impedir que estos puedan inhibir el crecimiento bacteriano. Una vez que ha pasado ese tiempo, se saca el escobillón del tubo de ensayo y se siembra en una placa de Cetrimida.For swabs, swabs are used Sterile that come inside a plastic test tube also sterile, this test tube carries 3 ml of Dey broth Engley Everything is done in sterile conditions. The Dey Engley impregnated swabs from the test tube and taken the sample dipping the swab in the maintenance solution containing the case and rub the walls of the case, to then they get back into the test tube containing 3 ml of sterile Dey Engley broth and left to act for ten minutes to neutralize the remains of antimicrobial agents of maintenance disinfectant solutions and prevent These can inhibit bacterial growth. Once you have after that time, the swab is removed from the test tube and sowing on a Cetrimide plate.
Una vez obtenidas éstas muestras se llevan a incubar a 42ºC durante 2 días.Once obtained these samples are taken to incubate at 42 ° C for 2 days.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200302640A ES2237316B1 (en) | 2003-11-12 | 2003-11-12 | KIT AND PROCEDURE TO IDENTIFY POLLUTION BY AERUGINOS PSEUDOMONAS OF SANITARY PRODUCTS. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200302640A ES2237316B1 (en) | 2003-11-12 | 2003-11-12 | KIT AND PROCEDURE TO IDENTIFY POLLUTION BY AERUGINOS PSEUDOMONAS OF SANITARY PRODUCTS. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2237316A1 ES2237316A1 (en) | 2005-07-16 |
| ES2237316B1 true ES2237316B1 (en) | 2006-12-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200302640A Expired - Fee Related ES2237316B1 (en) | 2003-11-12 | 2003-11-12 | KIT AND PROCEDURE TO IDENTIFY POLLUTION BY AERUGINOS PSEUDOMONAS OF SANITARY PRODUCTS. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2237316B1 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5863382A (en) * | 1981-10-13 | 1983-04-15 | Terumo Corp | Multi-layer culture test tool for microorganism |
| IT226753Z2 (en) * | 1992-07-08 | 1997-07-01 | Diesse Diagnostica | DEVICE FOR THE STORAGE AND ANALYSIS OF SAMPLES IN PARTICULAR FOR BACTERIOLOGICAL EXAMINATIONS ISOLATION OF MICRO-ORGANISMS AND DEVELOPMENT OF ISOLATION COLONIES |
| DE10034647C1 (en) * | 2000-07-14 | 2002-04-04 | 3M Espe Ag | Procedure for performing saliva analysis |
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2003
- 2003-11-12 ES ES200302640A patent/ES2237316B1/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| ES2237316A1 (en) | 2005-07-16 |
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