ES2212900B1 - DEVICE AND METHOD FOR TAKING SAMPLES OF MICROORGANISMS. - Google Patents

DEVICE AND METHOD FOR TAKING SAMPLES OF MICROORGANISMS.

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Publication number
ES2212900B1
ES2212900B1 ES200202706A ES200202706A ES2212900B1 ES 2212900 B1 ES2212900 B1 ES 2212900B1 ES 200202706 A ES200202706 A ES 200202706A ES 200202706 A ES200202706 A ES 200202706A ES 2212900 B1 ES2212900 B1 ES 2212900B1
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microorganisms
grid
petri dish
air
culture medium
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ES2212900A1 (en
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Miguel Lleonart Aliberas
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IUL SA
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IUL SA
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Priority to PCT/ES2003/000578 priority patent/WO2004048509A1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • G01N1/2214—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling by sorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2273—Atmospheric sampling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/26—Devices for withdrawing samples in the gaseous state with provision for intake from several spaces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a micro-organism sampling device and method. The inventive method involves the use of Petri dishes (4) which are positioned beneath a screen (1) comprising numerous holes (2). Moreover, all of aforementioned components are mounted in an air suction device, such that a column of air passes through the screen at a high velocity and falls on the culture medium (3) inside the Petri dish (4). After a certain period of time, the micro-organisms captured by the culture medium (3) enter the count process. The columns of air (6) associated with each of the holes (12) hit the culture medium (3) under pressure. In this way, cavities (7) are formed together with a massive accumulation of micro-organisms, which produces an uneven distribution over the whole surface and necessitates the use of complicated correction factors in order to minimise evaluation errors. According to the invention, totally uniform distribution is obtained through the continuous uniform rotation of the Petri dish (4) while the screen (1) remains fixed, or vice-versa.

Description

Dispositivo y método para toma de muestras de microorganismos.Device and method for sampling microorganisms

Objeto de la invenciónObject of the invention

La presente invención, según lo expresa el enunciado de esta memoria descriptiva, se refiere a un dispositivo y método para toma de muestras de microorganismos, que presenta unas importantes ventajas respecto a los sistemas convencionales, pues evita la aplicación de complejos factores de corrección a la hora de hacer el recuento de los microorganismos captados del aire.The present invention, as expressed by the set forth in this specification, refers to a device and method for sampling microorganisms, which presents important advantages over conventional systems, it avoids the application of complex correction factors to the time to count the microorganisms captured from air.

De esta forma se logra un recuento medida más fiel de dichos microorganismos.This way a more measured count is achieved faithful of these microorganisms.

En la actualidad, es habitual efectuar periódicamente recuento de los microorganismos existentes en determinados ambientes, como por ejemplo quirófanos, salas donde se manipulan alimentos, etc.At present, it is usual to perform Periodically count the existing microorganisms in certain environments, such as operating rooms, rooms where  handle food, etc.

Antecedentes de la invenciónBackground of the invention

El recuento de microorganismos se efectúa actualmente utilizando las cápsulas Petri, que son situadas debajo de una rejilla y todo ello montado en un dispositivo que succiona aire, de manera que a través de los orificios de dicha rejilla, se hace incidir a gran velocidad una columna de aire contra un medio de cultivo existente en el interior de dicha cápsula Petri.The count of microorganisms is carried out currently using the Petri capsules, which are located below of a grid and all mounted on a sucking device air, so that through the holes of said grid, makes a column of air strike at high speed against a medium of culture existing inside said Petri dish.

Transcurrido un tiempo, los organismos captados por el medio de cultivo pasan al proceso de recuento para determinar los parámetros perseguidos en esta operación.After a while, the organisms captured through the culture medium they go to the counting process to Determine the parameters pursued in this operation.

Las diminutas columnas de aire formadas por la succión al paso por los orificios de la rejilla, crean en el medio de cultivo una especie de rehundidos en los puntos de incisión y una acumulación masiva de microorganismos en estas zonas puntuales de toda la superficie de cultivo.The tiny air columns formed by the suction to the passage through the holes of the grid, create in the middle of cultivation a kind of sunken at the incision points and a massive accumulation of microorganisms in these specific areas of the entire cultivation area.

Para determinar la cantidad de microorganismos captados en función del volumen del aire que se ha empleado, es necesario introducir unos complicados factores de corrección, que desestime el error que puede producirse al no existir un reparto regular de los microorganismos en toda la superficie del medio de cultivo.To determine the amount of microorganisms captured according to the volume of air that has been used, is it is necessary to introduce some complicated correction factors, which dismiss the error that may occur when there is no cast regulate microorganisms on the entire surface of the medium of culture.

Descripción de la invenciónDescription of the invention

En líneas generales, el dispositivo y método para toma de muestras de microorganismos, objeto de la invención, consiguen evitar esta lectura estimada y obtener por lo tanto una lectura real, utilizando un dispositivo que imprima movimiento de giro continuado a la cápsula Petri, de un valor aproximadamente de dos vueltas por minuto, aunque puede variar en función de necesidades.In general, the device and method for Sampling of microorganisms, object of the invention, they manage to avoid this estimated reading and therefore obtain a actual reading, using a device that prints movement of Continuous rotation of the Petri dish, with a value of approximately two turns per minute, although it may vary depending on needs

Al estar girando la cápsula Petri, cada orificio de la rejilla efectúa una proyección de aire sobre el medio de cultivo, que produce un barrido según una corona circular que varía según la posición excéntrica que ocupa dicho orificio, de manera que los microorganismos son depositados ya no en puntos del medio de cultivo, sino en superficies con dicha forma geométrica.As the Petri dish is rotating, each hole of the grid makes an air projection on the middle of crop, which produces a sweep according to a circular crown that varies according to the eccentric position occupied by said hole, so that microorganisms are deposited no longer at middle points of cultivation, but on surfaces with said geometric shape.

Si a esto unimos una especial distribución de los orificios de la rejilla, de forma que la suma de todos sus barridos cubran perfectamente la superficie del medio de cultivo, se obtiene una distribución absolutamente regular y uniforme de dichos microorganismos.If we add to this a special distribution of  holes in the grid, so that the sum of all your sweeps perfectly cover the surface of the culture medium, an absolutely regular and uniform distribution of said microorganisms.

También se puede lograr el mismo efecto haciendo girar, en lugar de la cápsula Petri, la propia rejilla, pues lo importante es que exista un movimiento relativo de giro uniforme, entre dichos elementos: rejilla y cápsula Petri.You can also achieve the same effect by doing turn, instead of the Petri dish, the grid itself, because important is that there is a relative motion of uniform rotation, between these elements: grid and Petri dish.

Obviamente, por cuestiones mecánicas, los orificios deben tener cierta separación entre sí, ya que de lo contrario, su fabricación sería dificultosa puesto que habría que efectuar orificios muy juntos, lo que debilitaría la estructura de la rejilla.Obviously, for mechanical reasons, the holes should have some separation from each other, because of what On the contrary, its manufacture would be difficult since it would be necessary make holes very close together, which would weaken the structure of the grid.

Para facilitar la comprensión de las características de la invención y formando parte integrante de esta memoria descriptiva, se acompañan unas hojas de planos, en cuyas figuras, con carácter ilustrativo y no limitativo se ha representado lo siguiente:To facilitate the understanding of characteristics of the invention and forming an integral part of this Descriptive report, accompanying some sheets of plans, in whose figures, with an illustrative and non-limiting nature represented the following:

Breve descripción de los dibujosBrief description of the drawings

Figura 1. Es una vista esquemática en alzado, para observar el paso del aire a través de los orificios de la rejilla dispuesta sobre la cápsula Petri, a consecuencia de la succión efectuada por un dispositivo de aspiración inferior, según la técnica actual.Figure 1. It is a schematic elevation view, to observe the passage of air through the holes in the grid arranged on the Petri dish, as a result of the suction carried out by a lower suction device, according to The current technique.

Figura 2. Es una vista parcial y esquemática, a mayor escala para observar la formación de unos rehundidos puntuales en la superficie del medio de cultivo vertido en la cápsula Petri, según la técnica actual.Figure 2. It is a partial and schematic view, a larger scale to observe the formation of recesses point on the surface of the culture medium poured into the Petri dish, according to the current technique.

Figura 3. Es una vista esquemática en planta, de la superficie de cultivo de la cápsula Petri, en la que se aprecia la proyección de la columna de aire que atraviesa uno de los orificios de la rejilla y que debido al giro relativo entre ésta última y la cápsula Petri, con el método objeto de la invención, los microorganismos se sitúan en una corona circular, en lugar de hacerlo puntualmente como sucede en la técnica anterior.Figure 3. It is a schematic plan view of the culture surface of the Petri capsule, in which it is appreciated the projection of the air column that crosses one of the holes of the grid and that due to the relative rotation between it last and the Petri dish, with the method object of the invention, the microorganisms are placed in a circular crown, instead of do it promptly as in the prior art.

Figura 4. Muestra cuatro ejemplos de distribución de los orificios en la rejilla, para lograr un barrido completo de toda la superficie del medio de cultivo, acorde con la invención.Figure 4. Shows four distribution examples of the holes in the grid, to achieve a complete sweep of the entire surface of the culture medium, according to the invention.

Figura 5. Es una vista en alzado seccionado, de un dispositivo para toma de muestras de microorganismos, construido con las características ventajosas que la invención propone, según el método de toma de muestras de microorganismos con cápsulas Petri, objeto de la invención.Figure 5. It is a sectioned elevation view of a device for sampling microorganisms, built with the advantageous features that the invention proposes, according to the method of sampling of microorganisms with Petri capsules, object of the invention.

Descripción de la forma de realización preferidaDescription of the preferred embodiment

Haciendo referencia a la numeración adoptada en las figuras, podemos ver cómo el dispositivo en el que se puede realizar el método para toma de muestras de microorganismos, que la invención propone, está basado en el sistema de captación convencional haciendo pasar el aire del medio ambiente del que se quieren tomar las muestras, a través de una rejilla (1) provista de orificios (2), incidiendo las columnas de aire sobre la superficie del medio de cultivo (3) contenido en la cápsula Petri (4), pasando este aire por efecto de la succión producida por un dispositivo de aspiración inferior, vinculado a la carcasa (5), tal como se muestra esquemáticamente en la figura 1.Referring to the numbering adopted in the figures, we can see how the device in which you can perform the method for sampling microorganisms, which the invention proposes, is based on the collection system conventional by passing air from the environment from which it they want to take the samples, through a grid (1) provided with holes (2), impacting the air columns on the surface of the culture medium (3) contained in the Petri dish (4), passing this air due to the effect of the suction produced by a device lower suction, linked to the housing (5), as schematically shown in figure 1.

En la figura 2 se observa claramente el efecto que produce el paso de las columnas de aire representadas por las flechas (6), en la superficie del medio de cultivo (3), dando lugar a una especie de rehundidos (7), con una acumulación masiva de microorganismos (8), y debido a que el reparto no es uniforme en toda la superficie, es por lo que se hace necesario el empleo de complicados factores de corrección para que el recuento sea lo más aproximado posible a la realidad.Figure 2 clearly shows the effect which produces the passage of air columns represented by the arrows (6), on the surface of the culture medium (3), giving rise to a kind of sunken (7), with a massive accumulation of microorganisms (8), and because the distribution is not uniform in The entire surface is why the use of complicated correction factors so that the count is the most approximate possible to reality.

Por lo tanto, en la técnica actual el aspecto que toma la cápsula Petri tras la captación de microorganismos seria el que presenta una superficie dotada de una pluralidad de pequeñas depresiones circulares o rehundidos con la distribución de los orificios de la rejilla, con un reparto muy irregular de los microorganismos en toda la superficie.Therefore, in the current technique the aspect that take the Petri dish after the capture of serious microorganisms which has a surface endowed with a plurality of small circular or recessed depressions with the distribution of holes in the grid, with a very irregular distribution of microorganisms throughout the surface.

Por el contrario, al imprimir un giro de aproximadamente dos vueltas por minuto, a la cápsula Petri (4), o bien, a la propia rejilla (1) pues como hemos indicado anteriormente se consigue el mismo efecto, tal y como se muestra en la figura 3, cada una de las columnas de aire pasante por su respectivo orificio (2) de la rejilla (1) efectúa un barrido (9) en forma de corona circular y los microorganismos se disponen a lo largo de esta corona circular (9), en lugar de hacerlo puntualmente en los rehundidos (7) de la técnica conocida. Por lo tanto, la suma de todos los barridos anulares producida por el paso de aire por todos los orificios (2) de la rejilla (1), cubren toda la superficie del medio de cultivo (3), con una distribución absolutamente regular y uniforme de dichos microorganismos por toda la superficie del medio de cultivo contenido en la cápsula Petri. El aspecto que toma la cápsula Petri tras la captación de microorganismos, contrariamente a lo comentado anteriormente en relación con la técnica actual, es el de una superficie continua y lisa, es decir, sin ningún tipo de rehundidos, ya que la presión del aire o impacto de las columnas de aire contra el medio de cultivo no se localiza en puntos fijos sino a lo largo de respectivas coronas circulares (9).On the contrary, when printing a turn of approximately two turns per minute, to the Petri dish (4), or well, to the grid itself (1) because as we have indicated previously the same effect is achieved, as shown in Figure 3, each of the columns of air through its respective hole (2) of the grid (1) scans (9) in circular crown shape and microorganisms are arranged at along this circular crown (9), instead of on time in recesses (7) of the known technique. Therefore the sum  of all the annular sweeps produced by the passage of air through all holes (2) of the grid (1) cover the entire surface of the culture medium (3), with an absolutely distribution regular and uniform of said microorganisms throughout the surface of the culture medium contained in the Petri dish. The aspect that take the Petri capsule after the uptake of microorganisms, contrary to what has been commented previously in relation to the current technique, is that of a continuous and smooth surface, that is, without any recesses, since the air pressure or impact of the air columns against the culture medium is not located at fixed points but along respective circular crowns (9).

En la figura 4 podemos ver diferentes ejemplos de distribuciones válidas de los orificios (2) de la rejilla (1), con las que se logra el efecto de hacer un barrido completo de toda la superficie del medio de cultivo (3). En la posición a) de la figura 1, los orificios (2) de la rejilla (1) adoptan una distribución ordenada que sigue una línea en espiral y a la vez achatada para conseguir el efecto de desfases circulares múltiples en el giro relativo dado a la rejilla (1) respecto de la cápsula Petri (4).In figure 4 we can see different examples of  valid distributions of the holes (2) of the grid (1), with which achieves the effect of making a complete sweep of all the surface of the culture medium (3). In position a) of the figure 1, the holes (2) of the grid (1) adopt a distribution ordered that follows a spiral line and at the same time flattened for achieve the effect of multiple circular lags in the turn relative given to the grid (1) with respect to the Petri capsule (4).

En la posición b) de esta figura 4, los orificios (2) presentan una distribución en general radial de líneas arqueadas en un mismo sentido de giro, cubriendo una superficie también elíptica. En las posiciones c) y d) se observan otras distribuciones para los orificios (2) en la rejilla (1), que cumplen también la condición de efectuar un barrido completo de toda la superficie del medio de cultivo, con una distribución uniforme de los microorganismos. Estas diferentes distribuciones de orificios se determinan fácilmente siguiendo un proceso matemático para calcular tanto el número de orificios necesarios, como su distribución en la rejilla.In position b) of this figure 4, the holes (2) present a general radial distribution of lines arched in the same direction of rotation, covering a surface also elliptical. In positions c) and d) others are observed distributions for the holes (2) in the grid (1), which they also fulfill the condition of carrying out a complete sweep of the entire surface of the culture medium, with a distribution Uniform microorganisms. These different distributions of holes are easily determined by following a mathematical process to calculate both the number of holes needed, and their grid distribution.

Haciendo ahora especial referencia a la figura 5, en ella se muestra de forma esquemática un dispositivo para toma de muestras de microorganismos, siguiendo el método de la invención, donde la cápsula Petri (4) se encuentra montada en un plato giratorio (10) conectado a un moto-reductor que aplica un giro continuado a la cápsula Petri (4). En este ejemplo representado, la rejilla (1) permanece fija y forma parte o es solidaria de la tapa (11) que cierra el recipiente o carcasa (12) fijada a la bancada (13) en cuyo interior se encuentra el elemento succionador (14) cuya embocadura abarca una amplia zona abierta de la base de dicha carcasa (12).Making special reference now to Figure 5, it shows schematically a device for taking of samples of microorganisms, following the method of the invention, where the Petri dish (4) is mounted on a plate swivel (10) connected to a motor-reducer that apply a continuous twist to the Petri dish (4). In this example represented, the grid (1) remains fixed and is part or is integral with the lid (11) that closes the container or housing (12) fixed to the bench (13) inside which is the element sucker (14) whose mouthpiece covers a wide open area of the base of said housing (12).

Claims (6)

1. Método para toma de muestras de microorganismos, ideado con la finalidad de evitar la aplicación de complejos factores de corrección al hacer el recuento de los microorganismos captados en determinados ambientes, mediante cápsulas Petri situadas debajo de una rejilla pasando el aire a través de los orificios de dicha rejilla mediante un elemento succionador y depositándose dichos microorganismos en el medio de cultivo dispuesto en la cápsula Petri, caracterizado porque consiste en aplicar un giro continuado a la cápsula Petri (4) manteniendo fija la rejilla (1), o viceversa, de forma que cada orificio (2) de la rejilla (1) efectúe una proyección de aire sobre el medio de cultivo (3) que produce un barrido en forma de corona circular (9), de manera que los microorganismos son depositados en superficies con dicha forma geométrica, habiéndose previsto que la totalidad de orificios (2) de la rejilla (1) tenga una distribución especial para que la suma de todos los barridos cubra la totalidad de la superficie del medio de cultivo para lograr una distribución absolutamente regular y uniforme de los microorganismos.1. Method for sampling microorganisms, designed to avoid the application of complex correction factors when counting the microorganisms captured in certain environments, using Petri dishes located under a grid by passing the air through the orifices of said grid by means of a suction element and said microorganisms being deposited in the culture medium disposed in the Petri dish, characterized in that it consists in applying a continuous rotation to the Petri dish (4) keeping the grid fixed (1), or vice versa, of so that each hole (2) of the grid (1) makes an air projection on the culture medium (3) that produces a circular crown-shaped sweep (9), so that the microorganisms are deposited on surfaces with said geometric shape, having provided that all holes (2) of the grid (1) have a special distribution so that the sum of all sweeps cover the entire surface of the culture medium to achieve an absolutely regular and uniform distribution of microorganisms. 2. Dispositivo para toma de muestras de microorganismos, del tipo de los que incluyen una carcasa soporte de la cápsula de Petri, cerrada por una tapa que materializa la rejilla de paso del aire medioambiental, estando comunicada una amplia zona de su base, con un dispositivo succionador de aire, caracterizado porque la base de la carcasa (12), en la zona de fijación o de asiento de la cápsula Petri (4), define un plato giratorio (10) conectado a un moto-reductor que mantiene un movimiento uniforme para que los microorganismos se depositen, por cada uno de los orificios (2), según una proyección anular (9), lo que conlleva un barrido total de la superficie de cultivo (3).2. Device for the sampling of microorganisms, of the type that include a support housing of the Petri dish, closed by a cover that materializes the ambient air passage grid, being communicated a large area of its base, with a air suction device, characterized in that the base of the housing (12), in the fixing or seating area of the Petri dish (4), defines a turntable (10) connected to a motor-reducer that maintains a uniform movement so that the microorganisms are deposited, by each of the holes (2), according to an annular projection (9), which implies a total sweep of the cultivation surface (3). 3. Dispositivo para toma de muestras de microorganismos, del tipo de los que incluyen una carcasa soporte de la cápsula Petri, cerrada por una tapa que materializa la rejilla de paso del medio ambiente, estando comunicada una amplia zona abierta de su base, con un dispositivo succionador del aire, caracterizado porque la tapa (11) de la carcasa (12) portadora de la rejilla (1) está materializada por un platillo giratorio conectado a un moto-reductor para producir un movimiento angular uniforme (del orden de dos vueltas/minuto) y proyectar así los microorganismos pasantes por cada orificio (2) de la rejilla (1), sobre respectivas zonas anulares (9), efectuándose un barrido uniforme sobre toda la superficie de cultivo (3).3. Device for sampling microorganisms, of the type that include a support housing of the Petri dish, closed by a cover that materializes the environmental grid, being connected a wide open area of its base, with a air suction device, characterized in that the cover (11) of the housing (12) carrying the grid (1) is materialized by a rotating plate connected to a motor reducer to produce a uniform angular movement (of the order of two turns / minute) and thus project the through microorganisms through each hole (2) of the grid (1), over respective annular areas (9), making a uniform scan over the entire crop surface (3). 4. Dispositivo para toma de muestras de microorganismos, según reivindicaciones 2 y 3, caracterizado porque la rejilla (1) está provista de pequeños orificios (2) de paso del aire, con una distribución uniforme siguiendo una línea en general espiral.4. Device for sampling microorganisms, according to claims 2 and 3, characterized in that the grid (1) is provided with small holes (2) for the passage of air, with a uniform distribution along a generally spiral line. 5. Dispositivo para toma de muestras de microorganismos, según reivindicaciones 2 y 3, caracterizado porque la rejilla (1) está provista de pequeños orificios (2) de paso con una distribución uniforme siguiendo una línea en general radial.5. Device for sampling microorganisms according to claims 2 and 3, characterized in that the grid (1) is provided with small holes (2) with a uniform distribution along a generally radial line. 6. Dispositivo para toma de muestras de microorganismos, según reivindicaciones 2 y 3, caracterizado porque la rejilla (1) está provista de pequeños orificios (2) de paso con una distribución uniforme siguiendo líneas en general elípticas.6. Device for sampling microorganisms, according to claims 2 and 3, characterized in that the grid (1) is provided with small holes (2) with a uniform distribution along generally elliptical lines.
ES200202706A 2002-11-25 2002-11-25 DEVICE AND METHOD FOR TAKING SAMPLES OF MICROORGANISMS. Expired - Fee Related ES2212900B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES200202706A ES2212900B1 (en) 2002-11-25 2002-11-25 DEVICE AND METHOD FOR TAKING SAMPLES OF MICROORGANISMS.
PCT/ES2003/000578 WO2004048509A1 (en) 2002-11-25 2003-11-14 Micro-organism sampling device and method

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DE102006044546A1 (en) * 2006-09-22 2008-04-03 Biotest Ag Air Sampler
USD775364S1 (en) 2012-10-26 2016-12-27 Seward Limited Blender
CN104946524A (en) * 2015-07-10 2015-09-30 苏州华达仪器设备有限公司 Sampling device capable of controlling planktonic bacteria automatically
GB201905931D0 (en) * 2019-04-29 2019-06-12 Pinpoint Scient Limited Improvements in air sampling devices
CN112877183B (en) * 2021-01-29 2021-09-07 武汉兰卫医学检验实验室有限公司 Medical science inspection microbiological culture apparatus

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GB804586A (en) * 1956-04-24 1958-11-19 Chirana Praha Np An apparatus for determining the number of microbes and other micro-organisms in theair
FR2779822B1 (en) * 1998-06-10 2000-09-08 Millipore Sa SAMPLING METHOD AND APPARATUS FOR MICROBIOLOGICAL AIR ANALYSIS

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