ES2193861A1 - Molecular identification of fusarium oxysporum consists of contacting of DNA with an enzymatic amplification reaction mixture employing specific initiators - Google Patents

Molecular identification of fusarium oxysporum consists of contacting of DNA with an enzymatic amplification reaction mixture employing specific initiators

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Publication number
ES2193861A1
ES2193861A1 ES200102843A ES200102843A ES2193861A1 ES 2193861 A1 ES2193861 A1 ES 2193861A1 ES 200102843 A ES200102843 A ES 200102843A ES 200102843 A ES200102843 A ES 200102843A ES 2193861 A1 ES2193861 A1 ES 2193861A1
Authority
ES
Spain
Prior art keywords
dna
fusarium oxysporum
reaction mixture
contacting
molecular identification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES200102843A
Other languages
Spanish (es)
Other versions
ES2193861B1 (en
Inventor
Gasco Maria Del Mar Jimenez
Diaz Rafael Manuel Jimenez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Newbiotechnic SA
Universidad de Cordoba
Original Assignee
Newbiotechnic SA
Universidad de Cordoba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Newbiotechnic SA, Universidad de Cordoba filed Critical Newbiotechnic SA
Priority to ES200102843A priority Critical patent/ES2193861B1/en
Publication of ES2193861A1 publication Critical patent/ES2193861A1/en
Application granted granted Critical
Publication of ES2193861B1 publication Critical patent/ES2193861B1/en
Anticipated expiration legal-status Critical
Withdrawn - After Issue legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The molecular identification of Fusarium oxysporum comprises contacting DNA of Fusarium oxysporum f. sp. ciceris or its pathogenic derivatives 0.5 and 6 with a reaction mixture. This gives enzymatic amplification of DNA by specific PCR via a pair of specific initiators with analysis of the amplification products.
ES200102843A 2001-12-20 2001-12-20 METHOD FOR THE MOLECULAR IDENTIFICATION OF FUSARIUM OXYSPORUM F. SP. CICERIS AND ITS PATHOGENIC RACES 0,5 AND 6 THROUGH SPECIFIC PCR. Withdrawn - After Issue ES2193861B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200102843A ES2193861B1 (en) 2001-12-20 2001-12-20 METHOD FOR THE MOLECULAR IDENTIFICATION OF FUSARIUM OXYSPORUM F. SP. CICERIS AND ITS PATHOGENIC RACES 0,5 AND 6 THROUGH SPECIFIC PCR.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200102843A ES2193861B1 (en) 2001-12-20 2001-12-20 METHOD FOR THE MOLECULAR IDENTIFICATION OF FUSARIUM OXYSPORUM F. SP. CICERIS AND ITS PATHOGENIC RACES 0,5 AND 6 THROUGH SPECIFIC PCR.

Publications (2)

Publication Number Publication Date
ES2193861A1 true ES2193861A1 (en) 2003-11-01
ES2193861B1 ES2193861B1 (en) 2005-03-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES200102843A Withdrawn - After Issue ES2193861B1 (en) 2001-12-20 2001-12-20 METHOD FOR THE MOLECULAR IDENTIFICATION OF FUSARIUM OXYSPORUM F. SP. CICERIS AND ITS PATHOGENIC RACES 0,5 AND 6 THROUGH SPECIFIC PCR.

Country Status (1)

Country Link
ES (1) ES2193861B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102242208A (en) * 2011-07-05 2011-11-16 福建出入境检验检疫局检验检疫技术中心 SCAR-PCR identification method of Agaricus bisporus No. 333 strain

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2143051T3 (en) * 1994-04-25 2000-05-01 Novartis Ag DETECTION OF FUNGAL PATHOGENS USING THE POLYMERASE CHAIN REACTION.
ES2152933T3 (en) * 1991-10-23 2001-02-16 Baylor College Medicine DETERMINATION OF FOOTPRINTS RELATING TO BACTERIAL VINTAGES USING AMPLIFICATION OF REPETITIVE DNA SEQUENCES.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2152933T3 (en) * 1991-10-23 2001-02-16 Baylor College Medicine DETERMINATION OF FOOTPRINTS RELATING TO BACTERIAL VINTAGES USING AMPLIFICATION OF REPETITIVE DNA SEQUENCES.
ES2143051T3 (en) * 1994-04-25 2000-05-01 Novartis Ag DETECTION OF FUNGAL PATHOGENS USING THE POLYMERASE CHAIN REACTION.

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GARC-A PEDRAJAS M.D. et al. A simple PCR-based method for the detection of the chickpea-wilt pathogen Fusarium oxysporum f.sp. ciceris in artificial and natural soils. European Journal of Plant Pathology, 1999, 105:251-259. *
JIM+NEZ-GASCO M. et al. Identification of pathogenic races 0, 1B/C, 5, and 6 of Fusarium oxysporum f.sp. ciceris with random amplified polymorphic DNA (RAPD). European Journal of Plant Pathology, 2001, 107:239-248. *
KELLY A. et al. Use of the genetic fingerprinting and random amplified polymorphic DNA to characterize pathotypes of Fusarium oxysporum f.sp. ciceris infecting chickpea. Phytopathology, 1994, 84 (11):1293-1298. *
KELLY A.G. et al. In planta polymerase-chain-reaction detection of the wilt-inducing pathotype of Fusarium oxysporum f.sp. ciceris in chickpea (Cicer arietinum L.). Physiological and Molecular Plant Pathology, 1998, 52:397-409. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102242208A (en) * 2011-07-05 2011-11-16 福建出入境检验检疫局检验检疫技术中心 SCAR-PCR identification method of Agaricus bisporus No. 333 strain
CN102242208B (en) * 2011-07-05 2013-02-13 福建出入境检验检疫局检验检疫技术中心 SCAR-PCR identification method of Agaricus bisporus No. 333 strain

Also Published As

Publication number Publication date
ES2193861B1 (en) 2005-03-01

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