ES2189888T3 - Metodos para eliminar interferencias debidas a deshidrogenasas endogenas en ensayos enzimaticos. - Google Patents
Metodos para eliminar interferencias debidas a deshidrogenasas endogenas en ensayos enzimaticos.Info
- Publication number
- ES2189888T3 ES2189888T3 ES96937302T ES96937302T ES2189888T3 ES 2189888 T3 ES2189888 T3 ES 2189888T3 ES 96937302 T ES96937302 T ES 96937302T ES 96937302 T ES96937302 T ES 96937302T ES 2189888 T3 ES2189888 T3 ES 2189888T3
- Authority
- ES
- Spain
- Prior art keywords
- dehydrogenase
- endogenous
- sample
- analyte
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
LA PRESENTE INVENCION ES UNA MEJORA EN UN METODO QUE UTILIZA UNA DESHIDROGENASA MARCADORA PARA DETECTAR LA PRESENCIA DE UN ANALITO EN UNA MUESTRA EN LA QUE LAS DESHIDROGENASAS ENDOGENAS PRESENTES EN LA MUESTRA INTERFIEREN EN LA DETECCION DEL ANALITO. LA MEJORA CONSISTE EN LA INCLUSION DE UN INHIBIDOR DE LA DESHIDROGENASA ENDOGENA EN EL MEDIO DE ENSAYO. LA MEJORA ES PARTICULARMENTE UTIL ES LA MEDIDA DE ALCOHOL ETILICO UTILIZANDO ALCOHOL DESHIDROGENASA COMO ENZIMA MARCADOR CUANDO EL LACTATO Y LA LACTATO DESHIDROGENASA PRESENTES EN LA MUESTRA INTERFIEREN EN LA DETECCION DEL ANALITO. INHIBIDORES PARTICULARMENTE EFECTIVOS QUE EVITAN ESTA INTERFERENCIA SON LO ACIDOS GRASOS, TALES COMO EL ACIDO AGARICO Y LAURICO, Y SALES DE SULFATO DE ALQUILO, TALES COMO EL DODECIL SULFATO SODICO Y EL DECIL SULFATO SODICO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US613495P | 1995-11-02 | 1995-11-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2189888T3 true ES2189888T3 (es) | 2003-07-16 |
Family
ID=21719474
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96937302T Expired - Lifetime ES2189888T3 (es) | 1995-11-02 | 1996-10-30 | Metodos para eliminar interferencias debidas a deshidrogenasas endogenas en ensayos enzimaticos. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0865501B1 (es) |
JP (1) | JP3658691B2 (es) |
CA (1) | CA2236307A1 (es) |
DE (1) | DE69625617T2 (es) |
ES (1) | ES2189888T3 (es) |
WO (1) | WO1997016562A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5861269A (en) * | 1996-11-01 | 1999-01-19 | Dade Behring Marburg Gmbh | Methods for removing interferences due to endogenous dehydrogenases in enzyme assays |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3167787D1 (en) * | 1980-04-09 | 1985-01-31 | Nyegaard & Co As | Method for the estimation of hydroxysteroids and reagent system therefor |
DE3303098A1 (de) * | 1983-01-31 | 1984-08-02 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und reagenz zur glucosebestimmung im haemolysierten blut |
JPS6091998A (ja) * | 1983-10-27 | 1985-05-23 | Yukio Shigeta | 体液及び尿中のd―3―ヒドロキシ酪酸の測定法並びにそのための測定試薬 |
US4937047A (en) * | 1986-04-01 | 1990-06-26 | Konishiroku Photo Industry Co., Ltd. | Analytical element |
IE920779A1 (en) * | 1991-03-13 | 1992-09-23 | Du Pont | Selective stabilization of lactate dehydrogenase isoenzyme¹ld1 by high molecular weight polyols |
-
1996
- 1996-10-30 WO PCT/EP1996/004725 patent/WO1997016562A1/en active IP Right Grant
- 1996-10-30 DE DE69625617T patent/DE69625617T2/de not_active Expired - Lifetime
- 1996-10-30 EP EP96937302A patent/EP0865501B1/en not_active Expired - Lifetime
- 1996-10-30 ES ES96937302T patent/ES2189888T3/es not_active Expired - Lifetime
- 1996-10-30 JP JP51707197A patent/JP3658691B2/ja not_active Expired - Lifetime
- 1996-10-30 CA CA 2236307 patent/CA2236307A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP3658691B2 (ja) | 2005-06-08 |
JP2001507206A (ja) | 2001-06-05 |
CA2236307A1 (en) | 1997-05-09 |
DE69625617D1 (de) | 2003-02-06 |
DE69625617T2 (de) | 2003-08-21 |
EP0865501A1 (en) | 1998-09-23 |
EP0865501B1 (en) | 2003-01-02 |
WO1997016562A1 (en) | 1997-05-09 |
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