ES2188384B1 - PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID. - Google Patents
PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID.Info
- Publication number
- ES2188384B1 ES2188384B1 ES200101441A ES200101441A ES2188384B1 ES 2188384 B1 ES2188384 B1 ES 2188384B1 ES 200101441 A ES200101441 A ES 200101441A ES 200101441 A ES200101441 A ES 200101441A ES 2188384 B1 ES2188384 B1 ES 2188384B1
- Authority
- ES
- Spain
- Prior art keywords
- human dna
- crevicular fluid
- gingival crevicular
- procedure
- obtaining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000003731 gingival crevicular fluid Anatomy 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000004458 analytical method Methods 0.000 abstract 2
- 208000034826 Genetic Predisposition to Disease Diseases 0.000 abstract 1
- 108010006785 Taq Polymerase Proteins 0.000 abstract 1
- 230000002745 absorbent Effects 0.000 abstract 1
- 239000002250 absorbent Substances 0.000 abstract 1
- 239000011543 agarose gel Substances 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 abstract 1
- 229960005542 ethidium bromide Drugs 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
- 238000012800 visualization Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Urology & Nephrology (AREA)
- Plant Pathology (AREA)
- Hematology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Procedimiento de obtención de ADN humano a partir de fluido crevicular gingival. Realiza la extracción de ADN humano, para su análisis mediante detección molecular, así como para el análisis de la susceptibilidad genética a cualquier enfermedad, sin afectar a la actividad de la Taq-polimerasa en la PCR, obteniéndose un producto de correcta visualización tras su electroforesis en gel de agarosa y mediante tinción con bromuro de etidio, así como sometible a digestión con enzimas de restricción (RFLP) para estudio alélico o a su secuenciación directa, con calidad diagnóstica, en base a realizar una cuidadosa obtención del fluido crevicular gingival mediante puntas o tiras de papel absorbente o un cepillo interdental, evitando la contaminación de la muestra con las propias células del operador.Procedure for obtaining human DNA from gingival crevicular fluid. It carries out the extraction of human DNA, for its analysis by molecular detection, as well as for the analysis of the genetic susceptibility to any disease, without affecting the activity of Taq polymerase in the PCR, obtaining a product of correct visualization after its electrophoresis in agarose gel and by staining with ethidium bromide, as well as subject to digestion with restriction enzymes (RFLP) for allelic study or to its direct sequencing, with diagnostic quality, based on careful preparation of gingival crevicular fluid by means of tips or strips of absorbent paper or an interdental brush, preventing contamination of the sample with the operator's own cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200101441A ES2188384B1 (en) | 2001-06-21 | 2001-06-21 | PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200101441A ES2188384B1 (en) | 2001-06-21 | 2001-06-21 | PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2188384A1 ES2188384A1 (en) | 2003-06-16 |
| ES2188384B1 true ES2188384B1 (en) | 2005-01-01 |
Family
ID=8498138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200101441A Expired - Fee Related ES2188384B1 (en) | 2001-06-21 | 2001-06-21 | PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2188384B1 (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5047328A (en) * | 1988-10-26 | 1991-09-10 | The Board Of Trustees Of The University Of Illinois | Method for determination of the type and severity of periodontal disease states |
| US6309827B1 (en) * | 1997-03-28 | 2001-10-30 | Orasure Technologies, Inc. | Simultaneous collection of DNA and non-nucleic analytes |
| ES2128265B1 (en) * | 1997-06-04 | 2000-03-01 | Euroespes S A | GENETIC KIT FOR THE MOLECULAR CHARACTERIZATION OF ALZHEIMER'S DISEASE. |
| WO2000050564A2 (en) * | 1999-02-26 | 2000-08-31 | Myriad Genetics, Inc. | Method for extracting dna from dried specimens |
-
2001
- 2001-06-21 ES ES200101441A patent/ES2188384B1/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| HARTY LC et al. Collection of buccal cell DNA using treated cards. Cancer Epidemiol. Biomarkers Prev., Mayo 2000, Vol. 9, Núm. 5, páginas 501-506. Páginas 501,502. * |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2188384A1 (en) | 2003-06-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20030616 Kind code of ref document: A1 |
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| FG2A | Definitive protection |
Ref document number: 2188384B1 Country of ref document: ES |
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| FD1A | Patent lapsed |
Effective date: 20100315 |