ES2183665A1 - Dna segments that are useful for selecting differentiated cell types and the applications thereof - Google Patents
Dna segments that are useful for selecting differentiated cell types and the applications thereofInfo
- Publication number
- ES2183665A1 ES2183665A1 ES009902251A ES9902251A ES2183665A1 ES 2183665 A1 ES2183665 A1 ES 2183665A1 ES 009902251 A ES009902251 A ES 009902251A ES 9902251 A ES9902251 A ES 9902251A ES 2183665 A1 ES2183665 A1 ES 2183665A1
- Authority
- ES
- Spain
- Prior art keywords
- cell types
- differentiated cell
- useful
- applications
- dna segments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
Abstract
The DNA segment comprises: (i) the regions contributing to forming and stabilizing the transcription factor complex of a cell type which is specifically expressed in one differentiated cell type and (ii) a selection gene that is functionally connected so that it is expressed when the DNA segment is recognized and transcribed by the transcription factor complex of the specific gene. The AND segment can be used to obtain and select in vitro differentiated cell types from precursor cells. Cell and tissue banks for therapeutic or research purposes can be created with the cell types obtained.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200102125A ES2208024B1 (en) | 1999-10-14 | 1999-10-14 | A METHOD FOR OBTAINING IN VITRO MOTHER CELLS OF INDIVIDUAL MAMMALS ADULTS. |
ES9902251A ES2183665B1 (en) | 1999-10-14 | 1999-10-14 | USEFUL DNA SEGMENTS TO SELECT DIFFERENTIATED CELLULAR TYPES AND THEIR APPLICATIONS. |
AU77913/00A AU7791300A (en) | 1999-10-14 | 2000-10-13 | Dna segments that are useful for selecting differentiated cell types and the applications thereof |
PCT/ES2000/000391 WO2001027266A1 (en) | 1999-10-14 | 2000-10-13 | Dna segments that are useful for selecting differentiated cell types and the applications thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9902251A ES2183665B1 (en) | 1999-10-14 | 1999-10-14 | USEFUL DNA SEGMENTS TO SELECT DIFFERENTIATED CELLULAR TYPES AND THEIR APPLICATIONS. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2183665A1 true ES2183665A1 (en) | 2003-03-16 |
ES2183665B1 ES2183665B1 (en) | 2004-08-01 |
Family
ID=8310234
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200102125A Expired - Fee Related ES2208024B1 (en) | 1999-10-14 | 1999-10-14 | A METHOD FOR OBTAINING IN VITRO MOTHER CELLS OF INDIVIDUAL MAMMALS ADULTS. |
ES9902251A Expired - Fee Related ES2183665B1 (en) | 1999-10-14 | 1999-10-14 | USEFUL DNA SEGMENTS TO SELECT DIFFERENTIATED CELLULAR TYPES AND THEIR APPLICATIONS. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200102125A Expired - Fee Related ES2208024B1 (en) | 1999-10-14 | 1999-10-14 | A METHOD FOR OBTAINING IN VITRO MOTHER CELLS OF INDIVIDUAL MAMMALS ADULTS. |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU7791300A (en) |
ES (2) | ES2208024B1 (en) |
WO (1) | WO2001027266A1 (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994024274A1 (en) * | 1993-04-21 | 1994-10-27 | The University Of Edinburgh | Isolation, selection and propagation of animal transgenic stem cells |
WO1998035022A1 (en) * | 1997-02-06 | 1998-08-13 | Osiris Therapeutics, Inc. | p21?CIP1 OR p27KIP1¿ EFFECTS ON THE REGULATION OF DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS |
WO1998044142A1 (en) * | 1997-04-01 | 1998-10-08 | The General Hospital Corporation | Molecular marker for muscle stem cells |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9401380D0 (en) * | 1994-01-25 | 1994-03-23 | Pharmaceutical Proteins Ltd | Embryonic cell isolation |
US5753506A (en) * | 1996-05-23 | 1998-05-19 | Cns Stem Cell Technology, Inc. | Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals |
AU729377B2 (en) * | 1997-10-23 | 2001-02-01 | Asterias Biotherapeutics, Inc. | Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture |
-
1999
- 1999-10-14 ES ES200102125A patent/ES2208024B1/en not_active Expired - Fee Related
- 1999-10-14 ES ES9902251A patent/ES2183665B1/en not_active Expired - Fee Related
-
2000
- 2000-10-13 WO PCT/ES2000/000391 patent/WO2001027266A1/en active Application Filing
- 2000-10-13 AU AU77913/00A patent/AU7791300A/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994024274A1 (en) * | 1993-04-21 | 1994-10-27 | The University Of Edinburgh | Isolation, selection and propagation of animal transgenic stem cells |
WO1998035022A1 (en) * | 1997-02-06 | 1998-08-13 | Osiris Therapeutics, Inc. | p21?CIP1 OR p27KIP1¿ EFFECTS ON THE REGULATION OF DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS |
WO1998044142A1 (en) * | 1997-04-01 | 1998-10-08 | The General Hospital Corporation | Molecular marker for muscle stem cells |
Non-Patent Citations (2)
Title |
---|
HERRERA, P.L. et al. "Two transgenic approaches to define the cell lineages in endocrine pancreas development", MOLECULAR AND CELULAR ENDOCRINOLOGY, 1998, Vol. 140, pßginas 45-50. Todo el documento. * |
JUN-HSIANG, L. et al. "A tissue-specific promoter that can drive a foreign gene to express in the suprabasal urothelial cells of transgenic mice", PROC. NATL. ACAD. SCI. USA, 1995, Vol. 92, pßginas 679-683. Todo el documento. * |
Also Published As
Publication number | Publication date |
---|---|
ES2208024B1 (en) | 2005-10-01 |
AU7791300A (en) | 2001-04-23 |
ES2208024A1 (en) | 2004-06-01 |
ES2183665B1 (en) | 2004-08-01 |
WO2001027266A1 (en) | 2001-04-19 |
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Legal Events
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Date of ref document: 20030316 Kind code of ref document: A1 |
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