ES2180385B2 - Metodo para la obtencion de embriones y plantas haploides de alcornoque. - Google Patents

Metodo para la obtencion de embriones y plantas haploides de alcornoque.

Info

Publication number
ES2180385B2
ES2180385B2 ES200001953A ES200001953A ES2180385B2 ES 2180385 B2 ES2180385 B2 ES 2180385B2 ES 200001953 A ES200001953 A ES 200001953A ES 200001953 A ES200001953 A ES 200001953A ES 2180385 B2 ES2180385 B2 ES 2180385B2
Authority
ES
Spain
Prior art keywords
obtaining
embryos
haploid
cork oak
pure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200001953A
Other languages
English (en)
Other versions
ES2180385A1 (es
Inventor
De La Vega Jose Anto Manzanera
Perez M Angeles Bueno
Meana Oscar Vicente
Garay M Aranzazu Gomez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INST NAC DE INVESTIGACION AGRA
Universidad Politecnica de Madrid
Universidad Politecnica de Valencia
Original Assignee
INST NAC DE INVESTIGACION AGRA
Universidad Politecnica de Madrid
Universidad Politecnica de Valencia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INST NAC DE INVESTIGACION AGRA, Universidad Politecnica de Madrid, Universidad Politecnica de Valencia filed Critical INST NAC DE INVESTIGACION AGRA
Priority to ES200001953A priority Critical patent/ES2180385B2/es
Publication of ES2180385A1 publication Critical patent/ES2180385A1/es
Application granted granted Critical
Publication of ES2180385B2 publication Critical patent/ES2180385B2/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Método para la obtención de embriones y plantas haploides de alcornoque. La presente invención se basa en una aplicación de la biotecnología vegetal a la mejora genética del alcornoque (Quercus Suber L.), dirigida a la obtención de plantas haploides u homozigóticas puras mediante embriogénesis gamética. El método consiste en la obtención de embriones haploides de alcornoque a partir de células gaméticas (microsporas o granos de polen), mediante un tratamiento de estrés para la obtención de plantas homozigótica puras (líneas puras) aplicables a la mejora genética del alcornoque. Los embriones gaméticos producidos se tratan con frío, para inducir su germinación. Después se pasan a medio para estimular el desarrollo del tallo y obtener la producción de plantas. Este procedimiento resuelve los problemas de lentitud en la obtención de líneas puras por métodos clásicos de mejora genética.
ES200001953A 2000-08-01 2000-08-01 Metodo para la obtencion de embriones y plantas haploides de alcornoque. Expired - Fee Related ES2180385B2 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200001953A ES2180385B2 (es) 2000-08-01 2000-08-01 Metodo para la obtencion de embriones y plantas haploides de alcornoque.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200001953A ES2180385B2 (es) 2000-08-01 2000-08-01 Metodo para la obtencion de embriones y plantas haploides de alcornoque.

Publications (2)

Publication Number Publication Date
ES2180385A1 ES2180385A1 (es) 2003-02-01
ES2180385B2 true ES2180385B2 (es) 2004-03-01

Family

ID=8494547

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200001953A Expired - Fee Related ES2180385B2 (es) 2000-08-01 2000-08-01 Metodo para la obtencion de embriones y plantas haploides de alcornoque.

Country Status (1)

Country Link
ES (1) ES2180385B2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2281248B1 (es) * 2005-07-08 2008-10-01 Consejo Superior Investigaciones Cientificas Metodo para la obtencion de proembriones haploides de olivo a partir de microsporas aisladas.
ES2355455B1 (es) * 2009-09-11 2012-02-07 Instituto Nacional De Investigaciones Y Tecnología Agraria Y Alimentaria Método de obtención de embriones haploides y plantas doble-haploides de encina.
CN102577952B (zh) * 2012-02-10 2013-06-19 中国林业科学研究院亚热带林业研究所 一种弗吉尼亚栎组织培养方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5322789A (en) * 1990-06-26 1994-06-21 Dekalb Plant Genetics Isolated microspore and anther culture of corn

Also Published As

Publication number Publication date
ES2180385A1 (es) 2003-02-01

Similar Documents

Publication Publication Date Title
AR055176A1 (es) Duplicacion de cromosomas en embriones haploides de maiz
DE602007012269D1 (de) Verfahren zur herstellung transgener pflanzen
CR8806A (es) Metodo para cultivo de plantas
BRPI0808715A8 (pt) Preparação e uso de explantes de embrião de planta para transformação
AR075545A1 (es) Plantas con caracteristicas modificadas de crecimiento y un metodo para obtenerlas
AR058989A1 (es) Metodos para producir celulas y plantas gramineas transgenicas
WO2006128707A8 (en) Method of producing haploid and doubled haploid plant embryos
Bhoite et al. Plant tissue culture: A review
ATE552343T1 (de) Endosperm-spezifische expression und/oder expression in keimenden embryos monokotyledoner pflanzen
CN106993533A (zh) 一种木薯脆性胚性愈伤组织的培养方法
AR078409A1 (es) Metodo para mejorar la germinacion de embriones a partir de semilla artificial
ES2180385B2 (es) Metodo para la obtencion de embriones y plantas haploides de alcornoque.
CN104396743A (zh) 一种甜瓜的快速繁殖方法及应用
ATE550931T1 (de) Verfahren und vorrichtung zur mikrovermehrung von pflanzen
UA94575C2 (ru) Способ продуцирования органически связанного витамина в
ATE413462T1 (de) Pflanzen mit verbesserten wachstumseigenschaften und methoden für deren herstellung
Isah et al. Enhanced in vitro seedling recovery in nothapodytes nimmoniana (J. Graham) mabberly
Musiał et al. Ovules anatomy of selected apomictic taxa from Asteraceae family
Sulusoglu et al. Effect of pretreatments on seed germination of Prunus laurocerasus L.(Cherry laurel)
Zainudin In Vitro selection of jatropha curcas linn. hybrids using polyethylene glycol to obtain drought tolerance character
PITOYO et al. Effects of encapsulation matrix on physical properties and germination viability of calcium-alginate encapsulated plbs of Grammatophyllum scriptum
Kapruwan et al. Effect of growth regulators on the in vitro multiplication of Dendrocalamus Hamiltonii
ATE275330T1 (de) Verbesserung des keimens von somatischen pflanzlichen embryonen durch vorbehandlung
Batukaev et al. IN VITRO BIOTECHNOLOGICAL TECHNIQUES FOR HEALTH IMPROVEMENT AND REPRODUCTION OF GRAPE
¿ edivá et al. Micropropagation of common ash clones resistant to fungus Hymenoscyphus fraxineus

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 20030201

Kind code of ref document: A1

FG2A Definitive protection

Ref document number: 2180385B2

Country of ref document: ES

FD2A Announcement of lapse in spain

Effective date: 20180807