ES2178643T3 - Separacion citometrica de flujo de alta velocidad de celulas de mamifero viables. - Google Patents

Separacion citometrica de flujo de alta velocidad de celulas de mamifero viables.

Info

Publication number
ES2178643T3
ES2178643T3 ES93921270T ES93921270T ES2178643T3 ES 2178643 T3 ES2178643 T3 ES 2178643T3 ES 93921270 T ES93921270 T ES 93921270T ES 93921270 T ES93921270 T ES 93921270T ES 2178643 T3 ES2178643 T3 ES 2178643T3
Authority
ES
Spain
Prior art keywords
cells
viable
high speed
speed flow
mammal cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93921270T
Other languages
English (en)
Inventor
Dennis T Sasaki
Den Engh Gerrit J Van
Anne-Marie Buckle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Systemix Inc
Original Assignee
University of California
Systemix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California, Systemix Inc filed Critical University of California
Application granted granted Critical
Publication of ES2178643T3 publication Critical patent/ES2178643T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Landscapes

  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Nonmetallic Welding Materials (AREA)

Abstract

POBLACIONES DE CELULAS VIABLES SE SEPARAN PARA PROPORCIONAR CONJUNTOS Y SUBCONJUNTOS DE CELULAS COMO CELULAS VIABLES DE ALTA PUREZA Y ELEVADO RENDIMIENTO BASADOS EN EL NUMERO DE CELULAS ORIGINALES PRESENTES EN LA MEZCLA. LA CITOMETRIA DE FLUJO A GRAN VELOCIDAD SE EMPLEA APROVECHANDO LAS CARACTERISTICAS LIGERAS DE LAS CELULAS PARA SEPARAR LAS CELULAS, EMPLEANDO LAS ELEVADAS PRESIONES Y LA ELEVADA RAPIDEZ DE FLUJO PARA REDUCIR EL TIEMPO DE CLASIFICACION.
ES93921270T 1992-09-03 1993-08-31 Separacion citometrica de flujo de alta velocidad de celulas de mamifero viables. Expired - Lifetime ES2178643T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US94009792A 1992-09-03 1992-09-03

Publications (1)

Publication Number Publication Date
ES2178643T3 true ES2178643T3 (es) 2003-01-01

Family

ID=25474220

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93921270T Expired - Lifetime ES2178643T3 (es) 1992-09-03 1993-08-31 Separacion citometrica de flujo de alta velocidad de celulas de mamifero viables.

Country Status (8)

Country Link
EP (1) EP0662124B1 (es)
AT (1) ATE219141T1 (es)
AU (1) AU681597B2 (es)
DE (1) DE69332024T2 (es)
DK (1) DK0662124T3 (es)
ES (1) ES2178643T3 (es)
PT (1) PT662124E (es)
WO (1) WO1994005775A1 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641457A (en) * 1995-04-25 1997-06-24 Systemix Sterile flow cytometer and sorter with mechanical isolation between flow chamber and sterile enclosure
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
CA2822983C (en) 2000-11-29 2017-05-09 Xy, Llc System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
JP4595067B2 (ja) 2002-08-01 2010-12-08 エックスワイ，エルエルシー 低圧精子細胞分離システム
CN100570360C (zh) 2002-08-15 2009-12-16 Xy公司 一种流式细胞仪及流式细胞计数方法
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305171T3 (da) 2003-03-28 2022-03-21 Inguran Llc Apparat og fremgangsmåder til tilvejebringelse af kønssorteret dyresæd
ES2541121T3 (es) 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
US7892725B2 (en) 2004-03-29 2011-02-22 Inguran, Llc Process for storing a sperm dispersion
AU2005266930B2 (en) 2004-07-22 2010-09-16 Inguran, Llc Process for enriching a population of sperm cells
US8270781B2 (en) 2005-10-28 2012-09-18 The Regents Of The University Of California Apparatus and method for improved optical detection of particles in fluid
US9645010B2 (en) 2009-03-10 2017-05-09 The Regents Of The University Of California Fluidic flow cytometry devices and methods
US9134221B2 (en) 2009-03-10 2015-09-15 The Regents Of The University Of California Fluidic flow cytometry devices and particle sensing based on signal-encoding
US10024819B2 (en) 2010-10-21 2018-07-17 The Regents Of The University Of California Microfluidics with wirelessly powered electronic circuits
CN110579435B (zh) 2012-10-15 2023-09-26 纳诺赛莱克特生物医药股份有限公司 颗粒分选的系统、设备和方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell

Also Published As

Publication number Publication date
DE69332024T2 (de) 2002-11-28
EP0662124B1 (en) 2002-06-12
AU681597B2 (en) 1997-09-04
ATE219141T1 (de) 2002-06-15
WO1994005775A1 (en) 1994-03-17
PT662124E (pt) 2002-10-31
DK0662124T3 (da) 2002-10-07
EP0662124A1 (en) 1995-07-12
DE69332024D1 (de) 2002-07-18
AU4842593A (en) 1994-03-29
EP0662124A4 (en) 1998-12-02

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