ES2172383B1 - Procedimiento pra la construccion de vectores retrovirales policistronicos. - Google Patents
Procedimiento pra la construccion de vectores retrovirales policistronicos.Info
- Publication number
- ES2172383B1 ES2172383B1 ES200000876A ES200000876A ES2172383B1 ES 2172383 B1 ES2172383 B1 ES 2172383B1 ES 200000876 A ES200000876 A ES 200000876A ES 200000876 A ES200000876 A ES 200000876A ES 2172383 B1 ES2172383 B1 ES 2172383B1
- Authority
- ES
- Spain
- Prior art keywords
- retroviral vectors
- construction
- procedure
- policistronic
- tetracistronic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Procedimiento para la construcción de vectores retrovirales policistrónicos, que consiste en tras construcción de vectores retrovirales bicistrónicos utilizando las regiones autocortantes 2A o {dl}1D2A del virus de la fiebre aftosa (FMDV) para la coexpresión de dos genes, se combinan esas estructuras con un tercer gen cuya traducción era dirigida por un IRES. La introducción de un cuarto gen detrás de un IRES diferente, permite conseguir vectores retrovirales tetracistrónicos, construyéndose tres vectores bicistrónicos con las secuencias 2A o {dl}1D2A, tres tricistrónicos y dos tetracistrónicos. La principal ventaja de estas construcciones está en su utilidad para coexpresar varios genes (hasta cuatro) a la vez.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200000876A ES2172383B1 (es) | 2000-04-06 | 2000-04-06 | Procedimiento pra la construccion de vectores retrovirales policistronicos. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200000876A ES2172383B1 (es) | 2000-04-06 | 2000-04-06 | Procedimiento pra la construccion de vectores retrovirales policistronicos. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2172383A1 ES2172383A1 (es) | 2002-09-16 |
| ES2172383B1 true ES2172383B1 (es) | 2003-12-16 |
Family
ID=8493056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200000876A Expired - Fee Related ES2172383B1 (es) | 2000-04-06 | 2000-04-06 | Procedimiento pra la construccion de vectores retrovirales policistronicos. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2172383B1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7485291B2 (en) | 2003-06-03 | 2009-02-03 | Cell Genesys, Inc. | Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof |
| EP1636360A4 (en) | 2003-06-03 | 2006-11-08 | Cell Genesys Inc | COMPOSITIONS AND METHODS FOR ENHANCED EXPRESSION OF RECOMBINANT POLYPEPTIDES FROM A SINGLE VECTOR USING A PEPTIDE CLEAVAGE SITE |
| CA2573656A1 (en) | 2004-07-13 | 2006-02-16 | Cell Genesys, Inc. | Aav vector compositions and methods for enhanced expression of immunoglobulins using the same |
-
2000
- 2000-04-06 ES ES200000876A patent/ES2172383B1/es not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2172383A1 (es) | 2002-09-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20020916 Kind code of ref document: A1 Effective date: 20020916 |
|
| FG2A | Definitive protection |
Ref document number: 2172383B1 Country of ref document: ES |
|
| FD2A | Announcement of lapse in spain |
Effective date: 20200724 |