ES2170229T3 - Procedimiento de deteccion bioquimica asociado en linea a tecnicas de separacion de flujo. - Google Patents

Procedimiento de deteccion bioquimica asociado en linea a tecnicas de separacion de flujo.

Info

Publication number
ES2170229T3
ES2170229T3 ES96911109T ES96911109T ES2170229T3 ES 2170229 T3 ES2170229 T3 ES 2170229T3 ES 96911109 T ES96911109 T ES 96911109T ES 96911109 T ES96911109 T ES 96911109T ES 2170229 T3 ES2170229 T3 ES 2170229T3
Authority
ES
Spain
Prior art keywords
affinity molecule
effluent
line
controlled amount
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96911109T
Other languages
English (en)
Inventor
Der Greef Jan Rijksunivers Van
Hubertus Rijksuniversitei Irth
Aaike Johannes Rijk Oosterkamp
Eva Saskia Mareike Lutz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SCREENTEC BV
Original Assignee
SCREENTEC BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SCREENTEC BV filed Critical SCREENTEC BV
Application granted granted Critical
Publication of ES2170229T3 publication Critical patent/ES2170229T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8429—Preparation of the fraction to be distributed adding modificating material
    • G01N2030/8435—Preparation of the fraction to be distributed adding modificating material for chemical reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/884—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample organic compounds

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN METODO DE DETECCION EN CADENA, QUE COMPRENDE EL ACOPLAMIENTO EN CADENA DE UNA TECNICA BIOQUIMICA Y UNA TECNICA DE FRACCIONAMIENTO, COMPRENDIENDO DICHO METODO LA ADICION DE UNA CANTIDAD CONTROLADA DE UNA MOLECULA AFIN AL EFLUENTE DE LA FASE DE FRACCIONAMEINTO, DE FORMA QUE REACCIONE CON LOS ANALITOS CONTENIDOS EN EL EFLUENTE, LA ADICION POSTERIOR DE UNA CANTIDAD CONTROLADA DE UN LIGANDO DETECTABLE, CAPAZ DE UNIRSE A LA MOLECULA AFIN, Y LA DETECCION DEL COMPLEJO FORMADO ENTRE EL LIGANDO DETECTABLE Y LA MOLECULA AFIN. ADEMAS, LA INVENCION SE REFIERE A UN METODO EN CADENA PARA LA SELECCION DE COMPUESTOS EN FUNCION DE SU CAPACIDAD DE UNION A UNA MOLECULA AFIN CONOCIDA. DICHO METODO COMPRENDE UNA FASE DE UNA FASE DE FRACCIONAMIENTO QUE PROPORCIONA UN EFLUENTE, LA ADICION DE UNA CANTIDAD CONTROLADA DE DICHA MOLECULA AFIN AL EFLUENTE Y EL TRANSCURSO DE UN TIEMPO DE CONTACTO SUFICIENTE PARA PERMITIR UNA REACCION O INTERACCION ENTRE LOS COMPUESTOS PRESENTES EN ELEFLUENTE, LA ADICION POSTERIOR DE UNA CANTIDAD CONTROLADA DE UN LIGANDO DETECTABLE CAPAZ DE UNIRSE A LA MOLECULA AFIN Y LA DETECCION DEL COMPLEJO FORMADO ENTRE EL LIGANDO DETECTABLE Y LA MOLECULA AFIN.
ES96911109T 1995-04-21 1996-04-19 Procedimiento de deteccion bioquimica asociado en linea a tecnicas de separacion de flujo. Expired - Lifetime ES2170229T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP95201029 1995-04-21
EP95202987 1995-11-03
PCT/NL1996/000173 WO1996033409A1 (en) 1995-04-21 1996-04-19 On-line coupling of biochemical detection to continuous-flow separation techniques
EP96911109A EP0821790B1 (en) 1995-04-21 1996-04-19 On-line coupling of biochemical detection to continuous-flow separation techniques

Publications (1)

Publication Number Publication Date
ES2170229T3 true ES2170229T3 (es) 2002-08-01

Family

ID=26139240

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96911109T Expired - Lifetime ES2170229T3 (es) 1995-04-21 1996-04-19 Procedimiento de deteccion bioquimica asociado en linea a tecnicas de separacion de flujo.

Country Status (10)

Country Link
EP (1) EP0821790B1 (es)
JP (1) JP3739399B2 (es)
AT (1) ATE212441T1 (es)
AU (1) AU5409296A (es)
DE (1) DE69618776T2 (es)
DK (1) DK0821790T3 (es)
ES (1) ES2170229T3 (es)
PT (1) PT821790E (es)
SI (1) SI0821790T1 (es)
WO (1) WO1996033409A1 (es)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5183740A (en) * 1990-02-23 1993-02-02 The United States Of America As Represented By The Secretary Of The Navy Flow immunosensor method and apparatus

Also Published As

Publication number Publication date
DE69618776D1 (de) 2002-03-14
DK0821790T3 (da) 2002-05-13
EP0821790B1 (en) 2002-01-23
ATE212441T1 (de) 2002-02-15
WO1996033409A1 (en) 1996-10-24
PT821790E (pt) 2002-07-31
AU5409296A (en) 1996-11-07
EP0821790A1 (en) 1998-02-04
JP3739399B2 (ja) 2006-01-25
JPH11509922A (ja) 1999-08-31
SI0821790T1 (en) 2002-04-30
DE69618776T2 (de) 2002-10-17

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