ES2169966B1 - KIT FOR SPECIFIC DIAGNOSIS OF LEISHMANIOSIS CAUSED BY "LEISHMANIA INFANTUM" BY THE PCR-ELISA TECHNIQUE. - Google Patents
KIT FOR SPECIFIC DIAGNOSIS OF LEISHMANIOSIS CAUSED BY "LEISHMANIA INFANTUM" BY THE PCR-ELISA TECHNIQUE.Info
- Publication number
- ES2169966B1 ES2169966B1 ES009901804A ES9901804A ES2169966B1 ES 2169966 B1 ES2169966 B1 ES 2169966B1 ES 009901804 A ES009901804 A ES 009901804A ES 9901804 A ES9901804 A ES 9901804A ES 2169966 B1 ES2169966 B1 ES 2169966B1
- Authority
- ES
- Spain
- Prior art keywords
- leishmaniosis
- pcr
- kit
- leishmania infantum
- diagnosis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Abstract
"Leishmania infantum" es el único agente etiológico de las leishmaniosis en España y la mayoría de los países mediterráneos europeos. Hay diversas técnicas usadas de forma habitual para el diagnóstico de las leishmaniosis, que sin embargo plantean una serie de problemas fundamentales, tanto en el diagnóstico de la leishmaniosis humana como de la leishmaniosis canina. El objetivo del "Kit para diagnóstico específico de las leishmaniosis causadas por Leishmania infantum por la técnica de PCR-ELISA" es mejorar la sensibilidad y especificidad de las técnicas de diagnóstico de las leishmaniosis usadas actualmente, superando los problemas planteados por éstas. El kit está basado en la utilización, en una PCR, de unos cebadores (9 y 83) que amplifican un fragmento específico de Leishmania infantum, y en una ELISA, de una sonda oligonucleótida interna, marcada con biotina, que hibrida también de forma específica con el fragmento amplificado."Leishmania infantum" is the only etiological agent of leishmaniasis in Spain and most European Mediterranean countries. There are various techniques commonly used for the diagnosis of leishmaniosis, which however pose a number of fundamental problems, both in the diagnosis of human leishmaniosis and canine leishmaniosis. The objective of the "Kit for specific diagnosis of leishmaniasis caused by Leishmania infantum by the PCR-ELISA technique" is to improve the sensitivity and specificity of the diagnostic techniques of leishmaniasis currently used, overcoming the problems posed by them. The kit is based on the use, in a PCR, of primers (9 and 83) that amplify a specific fragment of Leishmania infantum, and in an ELISA, of an internal oligonucleotide probe, labeled with biotin, which also hybridizes specifically with the amplified fragment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES009901804A ES2169966B1 (en) | 1999-08-03 | 1999-08-03 | KIT FOR SPECIFIC DIAGNOSIS OF LEISHMANIOSIS CAUSED BY "LEISHMANIA INFANTUM" BY THE PCR-ELISA TECHNIQUE. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES009901804A ES2169966B1 (en) | 1999-08-03 | 1999-08-03 | KIT FOR SPECIFIC DIAGNOSIS OF LEISHMANIOSIS CAUSED BY "LEISHMANIA INFANTUM" BY THE PCR-ELISA TECHNIQUE. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2169966A1 ES2169966A1 (en) | 2002-07-16 |
| ES2169966B1 true ES2169966B1 (en) | 2003-11-01 |
Family
ID=8309559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES009901804A Expired - Fee Related ES2169966B1 (en) | 1999-08-03 | 1999-08-03 | KIT FOR SPECIFIC DIAGNOSIS OF LEISHMANIOSIS CAUSED BY "LEISHMANIA INFANTUM" BY THE PCR-ELISA TECHNIQUE. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2169966B1 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08275799A (en) * | 1995-04-04 | 1996-10-22 | Sumitomo Electric Ind Ltd | Polynucleotide for detecting leishmaniasis and detection of leishmania protozoa |
-
1999
- 1999-08-03 ES ES009901804A patent/ES2169966B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2169966A1 (en) | 2002-07-16 |
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Legal Events
| Date | Code | Title | Description |
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| EC2A | Search report published |
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| FD2A | Announcement of lapse in spain |
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