ES2167161B1 - GENES SUPER EXPRESSION PROCEDURE REGULATED BY A GENETIC CIRCUIT IN CASCADA. - Google Patents

GENES SUPER EXPRESSION PROCEDURE REGULATED BY A GENETIC CIRCUIT IN CASCADA.

Info

Publication number
ES2167161B1
ES2167161B1 ES9901383A ES9901383A ES2167161B1 ES 2167161 B1 ES2167161 B1 ES 2167161B1 ES 9901383 A ES9901383 A ES 9901383A ES 9901383 A ES9901383 A ES 9901383A ES 2167161 B1 ES2167161 B1 ES 2167161B1
Authority
ES
Spain
Prior art keywords
genetic circuit
genes
regulated
cascada
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9901383A
Other languages
Spanish (es)
Other versions
ES2167161A1 (en
Inventor
Ramirez Angel Cebolla
Martin Carolina Sousa
Lorendo Prieto Victor De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9901383A priority Critical patent/ES2167161B1/en
Priority to ES200001389A priority patent/ES2181534B1/en
Priority to AT00938953T priority patent/ATE278786T1/en
Priority to PCT/IB2000/000830 priority patent/WO2000078976A1/en
Priority to ES00938953T priority patent/ES2228541T3/en
Priority to DE60014640T priority patent/DE60014640T2/en
Priority to EP00938953A priority patent/EP1190076B1/en
Priority to CA002375817A priority patent/CA2375817A1/en
Priority to AU54172/00A priority patent/AU5417200A/en
Priority to US09/746,567 priority patent/US6803224B2/en
Publication of ES2167161A1 publication Critical patent/ES2167161A1/en
Application granted granted Critical
Publication of ES2167161B1 publication Critical patent/ES2167161B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Abstract

Procedimiento de superexpresión de genes regulada por un circuito genético en cascada. El objeto de la presente invención es un procedimiento de superexpresión de genes regulada por un circuito genético en cascada en células transformadas. El procedimiento permite mantener unos valores basales muy reducidos y utiliza un sistema de expresión primario nahR/Psal que controla un gen regulador secundario xylS2 que estando situado en un vector movilizable y dentro de secuencias transponibles, puede establecerse en el cromosoma de una gran variedad de bacterias Gram negativas de forma estable, así como un sistema de expresión secundario que contiene el promotor Pm, diana de xylS2, y sitios múltiples de clonación donde pueden insertarse los genes de interés. Esta construcción podrá situarse en el cromosoma o en plásmidos de cepas que contengan el sistema regulador primario. La activación del sistema se hace con compuestos aromáticos derivados del ácido benzoico.Gene overexpression procedure regulated by a cascade genetic circuit. The object of the present invention is a gene overexpression procedure regulated by a cascaded genetic circuit in transformed cells. The procedure allows to maintain very low baseline values and uses a nahR / Psal primary expression system that controls a secondary regulatory gene xylS2 that being located in a mobilizable vector and within transposable sequences, can be established on the chromosome of a wide variety of bacteria Gram-negative stably, as well as a secondary expression system that contains the Pm promoter, xylS2 target, and multiple cloning sites where the genes of interest can be inserted. This construct may be located on the chromosome or plasmid strains containing the primary regulatory system. The activation of the system is done with aromatic compounds derived from benzoic acid.

ES9901383A 1999-06-22 1999-06-22 GENES SUPER EXPRESSION PROCEDURE REGULATED BY A GENETIC CIRCUIT IN CASCADA. Expired - Fee Related ES2167161B1 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
ES9901383A ES2167161B1 (en) 1999-06-22 1999-06-22 GENES SUPER EXPRESSION PROCEDURE REGULATED BY A GENETIC CIRCUIT IN CASCADA.
ES200001389A ES2181534B1 (en) 1999-06-22 2000-05-31 CERTIFICATE OF ADDITION OF THE PATENT 9901383: REGULATED EXPRESSION OF GENES USING A GENETIC CIRCUIT IN CASCADA.
PCT/IB2000/000830 WO2000078976A1 (en) 1999-06-22 2000-06-22 Regulated expression of cloned genes using a cascade genetic circuit
ES00938953T ES2228541T3 (en) 1999-06-22 2000-06-22 REGULATED EXPRESSION OF CLONE GENES USING A GENETIC CYCLE IN CASCADA.
DE60014640T DE60014640T2 (en) 1999-06-22 2000-06-22 GENETIC CASCADE REGULATED EXPRESSION OF CLONED GENES
EP00938953A EP1190076B1 (en) 1999-06-22 2000-06-22 Regulated expression of cloned genes using a cascade genetic circuit
AT00938953T ATE278786T1 (en) 1999-06-22 2000-06-22 GENETIC CAScade-regulated expression of cloned genes
CA002375817A CA2375817A1 (en) 1999-06-22 2000-06-22 Regulated expression of cloned genes using a cascade genetic circuit
AU54172/00A AU5417200A (en) 1999-06-22 2000-06-22 Regulated expression of cloned genes using a cascade genetic circuit
US09/746,567 US6803224B2 (en) 1999-06-22 2000-12-21 Regulated expression of cloned genes using a cascade genetic circuit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9901383A ES2167161B1 (en) 1999-06-22 1999-06-22 GENES SUPER EXPRESSION PROCEDURE REGULATED BY A GENETIC CIRCUIT IN CASCADA.

Publications (2)

Publication Number Publication Date
ES2167161A1 ES2167161A1 (en) 2002-05-01
ES2167161B1 true ES2167161B1 (en) 2003-07-16

Family

ID=8308930

Family Applications (2)

Application Number Title Priority Date Filing Date
ES9901383A Expired - Fee Related ES2167161B1 (en) 1999-06-22 1999-06-22 GENES SUPER EXPRESSION PROCEDURE REGULATED BY A GENETIC CIRCUIT IN CASCADA.
ES200001389A Expired - Fee Related ES2181534B1 (en) 1999-06-22 2000-05-31 CERTIFICATE OF ADDITION OF THE PATENT 9901383: REGULATED EXPRESSION OF GENES USING A GENETIC CIRCUIT IN CASCADA.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES200001389A Expired - Fee Related ES2181534B1 (en) 1999-06-22 2000-05-31 CERTIFICATE OF ADDITION OF THE PATENT 9901383: REGULATED EXPRESSION OF GENES USING A GENETIC CIRCUIT IN CASCADA.

Country Status (1)

Country Link
ES (2) ES2167161B1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8901677D0 (en) * 1989-01-26 1989-03-15 Ici Plc Hybrid seed production
EP0455424A3 (en) * 1990-05-02 1992-04-29 Merck & Co. Inc. Mammalian inducible promoter cascade system
GB9618001D0 (en) * 1996-08-29 1996-10-09 Nyfotek As Novel expression vectors

Also Published As

Publication number Publication date
ES2181534A1 (en) 2003-02-16
ES2181534B1 (en) 2004-06-16
ES2167161A1 (en) 2002-05-01

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