ES2160019B1 - Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens. - Google Patents

Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens.

Info

Publication number
ES2160019B1
ES2160019B1 ES9801920A ES9801920A ES2160019B1 ES 2160019 B1 ES2160019 B1 ES 2160019B1 ES 9801920 A ES9801920 A ES 9801920A ES 9801920 A ES9801920 A ES 9801920A ES 2160019 B1 ES2160019 B1 ES 2160019B1
Authority
ES
Spain
Prior art keywords
feed
tomato
cotyledon
nutrients
propagation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9801920A
Other languages
English (en)
Other versions
ES2160019A1 (es
Inventor
Lapuerta Jesus Chamarro
Romero Javier Pozueta
Rodolphe Schantz
Guy Houlne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Centre National de la Recherche Scientifique CNRS
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Centre National de la Recherche Scientifique CNRS filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9801920A priority Critical patent/ES2160019B1/es
Publication of ES2160019A1 publication Critical patent/ES2160019A1/es
Application granted granted Critical
Publication of ES2160019B1 publication Critical patent/ES2160019B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Abstract

La invención se basa en que, durante la germinación, el endospermo y el embrión poseen los nutrientes y, particularmente, los reguladores del crecimiento, necesarios para el desarrollo de la planta. A medida que avanza la germinación el agotamiento de dichos nutrientes se sustituye por la progresiva producción de los mismos por los cotiledones y la radícula. La eliminación del meristemo primario y los secundarios, conservando un cotiledón y la radícula, priva al explanto de los órganos necesarios para continuar el crecimiento vegetativo. En la zona de corte se forma un callo con células indiferenciadas totipotentes. La aportación de nutrientes y reguladores del crecimiento por parte del cotiledón y de la radícula proporciona los elementos que estimulan la diferenciación de las células del callo para la formación de yemas y tallos. A partir de los mismos se forman las raíces y, finalmente, se obtiene una planta adulta.
ES9801920A 1998-09-11 1998-09-11 Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens. Expired - Fee Related ES2160019B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9801920A ES2160019B1 (es) 1998-09-11 1998-09-11 Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9801920A ES2160019B1 (es) 1998-09-11 1998-09-11 Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens.

Publications (2)

Publication Number Publication Date
ES2160019A1 ES2160019A1 (es) 2001-10-16
ES2160019B1 true ES2160019B1 (es) 2002-05-01

Family

ID=8305143

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9801920A Expired - Fee Related ES2160019B1 (es) 1998-09-11 1998-09-11 Procedimiento para la propagacion vegetativa y transformacion genetica de tomate, pimiento y otras dicotiledoneas, mediante agrobacterium tumefaciens.

Country Status (1)

Country Link
ES (1) ES2160019B1 (es)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7335187A (en) * 1986-06-10 1987-12-17 Calgene, Inc. Agrobacterium transformation of plant tissue
IL88266A (en) * 1987-11-18 1998-03-10 Phytogen A method for regenerating a cotton plant from somatic cotton cells
EP0419533A1 (en) * 1988-06-01 1991-04-03 THE TEXAS A&M UNIVERSITY SYSTEM Method for transforming plants via the shoot apex
EP0582603A4 (en) * 1991-04-01 1995-09-27 Florigene B V Carnation plants and methods for their transformation and propagation
US5262316A (en) * 1991-11-22 1993-11-16 Dna Plant Technology Corporation Genetically transformed pepper plants and methods for their production

Also Published As

Publication number Publication date
ES2160019A1 (es) 2001-10-16

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