ES2153768A1 - Secuenciador perfeccionado de proteinas y acidos nucleicos, por electroforesis, y procecimiento para su operacion. - Google Patents

Secuenciador perfeccionado de proteinas y acidos nucleicos, por electroforesis, y procecimiento para su operacion.

Info

Publication number
ES2153768A1
ES2153768A1 ES009900529A ES9900529A ES2153768A1 ES 2153768 A1 ES2153768 A1 ES 2153768A1 ES 009900529 A ES009900529 A ES 009900529A ES 9900529 A ES9900529 A ES 9900529A ES 2153768 A1 ES2153768 A1 ES 2153768A1
Authority
ES
Spain
Prior art keywords
sequencer
operating
nucleic acid
same
improved protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009900529A
Other languages
English (en)
Other versions
ES2153768B1 (es
Inventor
Tudela Bernardo Hernandez
Amigo Vicente M Villar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to ES9900529A priority Critical patent/ES2153768B1/es
Priority to AU36594/00A priority patent/AU3659400A/en
Priority to PCT/ES2000/000089 priority patent/WO2000055615A1/es
Publication of ES2153768A1 publication Critical patent/ES2153768A1/es
Application granted granted Critical
Publication of ES2153768B1 publication Critical patent/ES2153768B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of Radiation (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

El secuenciador perfeccionado de proteínas y ácidos nucleicos por electroforesis, y procedimiento para su operación, que constituye el objeto de esta Patente, realiza dicho proceso en el seno de un gas inerte. Su estructura se compone de: una cámara oscura (1) con dos electrodos (2)-(2'') en planos opuestos, alimentación de gas (3) e inyección de la muestra acuosa mediante un electro-spray (4); un sistema electrónico de adquisición de datos, con ordenador (6); y una fuente de alimentación de corriente continua (7) con doble conmutador (8) para cambio de polaridad de los electrodos. En la utilización del secuenciador con radiactividad, las moléculas son marcadas con un radioisótopo, llegando al segundo electrodo compuesto por dos planchas (10)-(10''), la frontal de orificios (11), entre las cuales se encuentra un centelleador (12), que facilita la detección de las moléculas mediante un fotodetector (13) que informa al ordenador (6). En la utilización sin radiactividad, el segundo electrodo esun detector eléctrico gaseoso (14).
ES9900529A 1999-03-15 1999-03-15 Secuenciador perfeccionado de proteinas y acidos nucleicos, por electroforesis, y procecimiento para su operacion. Expired - Lifetime ES2153768B1 (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES9900529A ES2153768B1 (es) 1999-03-15 1999-03-15 Secuenciador perfeccionado de proteinas y acidos nucleicos, por electroforesis, y procecimiento para su operacion.
AU36594/00A AU3659400A (en) 1999-03-15 2000-03-15 Improved protein and nucleic acid electrophoresis sequencer and method for operating the same
PCT/ES2000/000089 WO2000055615A1 (es) 1999-03-15 2000-03-15 Secuenciador perfeccionado de proteinas y acidos nucleicos por electroforesis y procedimiento operacional

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9900529A ES2153768B1 (es) 1999-03-15 1999-03-15 Secuenciador perfeccionado de proteinas y acidos nucleicos, por electroforesis, y procecimiento para su operacion.

Publications (2)

Publication Number Publication Date
ES2153768A1 true ES2153768A1 (es) 2001-03-01
ES2153768B1 ES2153768B1 (es) 2001-11-01

Family

ID=8307671

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9900529A Expired - Lifetime ES2153768B1 (es) 1999-03-15 1999-03-15 Secuenciador perfeccionado de proteinas y acidos nucleicos, por electroforesis, y procecimiento para su operacion.

Country Status (3)

Country Link
AU (1) AU3659400A (es)
ES (1) ES2153768B1 (es)
WO (1) WO2000055615A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT500613B1 (de) * 2004-09-22 2006-02-15 Red Bull Gmbh Verfahren zur diagnostizierung neurodegenerativer störungen
CN102866194B (zh) * 2012-09-05 2014-04-02 上海市东方医院 多元电泳判读仪

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4146454A (en) * 1970-12-28 1979-03-27 Haber Instruments, Inc. Electromolecular propulsion in diverse semiconductive media
US5240578A (en) * 1991-09-24 1993-08-31 Shimadzu Corporation Capillary electrophoresis system and method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4146454A (en) * 1970-12-28 1979-03-27 Haber Instruments, Inc. Electromolecular propulsion in diverse semiconductive media
US5240578A (en) * 1991-09-24 1993-08-31 Shimadzu Corporation Capillary electrophoresis system and method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
REIMANN, F. et al. "Gas-phase sequencing of peptides and proteins". En: Advanced methods in protein microsequence analysis. Editado por B. Wittmann-Liebeld et al. Berlin: Springer, 1986, paginas 118-125. Todo el documento. *

Also Published As

Publication number Publication date
WO2000055615A1 (es) 2000-09-21
ES2153768B1 (es) 2001-11-01
AU3659400A (en) 2000-10-04

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