ES2151354A1 - Fabricacion de columnas capilares internamente recubiertas de polimeros para electroforesis capilar. - Google Patents

Fabricacion de columnas capilares internamente recubiertas de polimeros para electroforesis capilar.

Info

Publication number
ES2151354A1
ES2151354A1 ES009701655A ES9701655A ES2151354A1 ES 2151354 A1 ES2151354 A1 ES 2151354A1 ES 009701655 A ES009701655 A ES 009701655A ES 9701655 A ES9701655 A ES 9701655A ES 2151354 A1 ES2151354 A1 ES 2151354A1
Authority
ES
Spain
Prior art keywords
fabrication
application
positive charge
capillary columns
osmotic flow
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009701655A
Other languages
English (en)
Other versions
ES2151354B1 (es
Inventor
Gallego Alejandro Cifuentes
Masa Jose Carlos Diez
Buj Gustavo Adolfo Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sugelabor S A
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Sugelabor S A
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sugelabor S A, Consejo Superior de Investigaciones Cientificas CSIC filed Critical Sugelabor S A
Priority to ES9701655A priority Critical patent/ES2151354B1/es
Publication of ES2151354A1 publication Critical patent/ES2151354A1/es
Application granted granted Critical
Publication of ES2151354B1 publication Critical patent/ES2151354B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)

Abstract

El desarrollo de la Electroforesis Capilar como técnica analítica, ha supuesto la posibilidad de su utilización en un gran número de nuevas aplicaciones. Algunas de estas aplicaciones, sobre todo en el campo de la biotecnología, suponen el uso de columnas capilares que garanticen el control del flujo electroosmótico, especialmente por dar lugar a flujo electroosmótico prácticamente nulo y flujo electroosmótico invertido, eliminando al mismo tiempo los efectos de adsorción entre las sustancias a analizar y la pared interna del capilar. En esta patente se propone un método para la obtención de columnas capilares recubiertas con polímeros que aseguran estos dos supuestos, tratándose de polímeros neutros hidrófilos en el primero y polímeros con carga positiva en el segundo. El método asegura la reproducibilidad de los resultados analíticos derivados de su uso.
ES9701655A 1997-07-28 1997-07-28 Fabricacion de columnas capilares internamente recubiertas de polimeros para electroforesis capilar. Expired - Lifetime ES2151354B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9701655A ES2151354B1 (es) 1997-07-28 1997-07-28 Fabricacion de columnas capilares internamente recubiertas de polimeros para electroforesis capilar.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9701655A ES2151354B1 (es) 1997-07-28 1997-07-28 Fabricacion de columnas capilares internamente recubiertas de polimeros para electroforesis capilar.

Publications (2)

Publication Number Publication Date
ES2151354A1 true ES2151354A1 (es) 2000-12-16
ES2151354B1 ES2151354B1 (es) 2001-07-01

Family

ID=8300190

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9701655A Expired - Lifetime ES2151354B1 (es) 1997-07-28 1997-07-28 Fabricacion de columnas capilares internamente recubiertas de polimeros para electroforesis capilar.

Country Status (1)

Country Link
ES (1) ES2151354B1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2156476A1 (es) * 1998-02-16 2001-06-16 Consejo Superior Investigacion Tratamientos de colummnas capilares de silice fundida para la obtencion de capilares funcionalizados con grupos vinilo.

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4680201A (en) * 1985-10-30 1987-07-14 Stellan Hjerten Coating for electrophoresis tube
EP0354984A2 (en) * 1988-08-19 1990-02-21 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow
EP0417925A2 (en) * 1989-09-12 1991-03-20 Northeastern University High performance microcapillary gel electrophoresis
WO1992005433A1 (fr) * 1990-09-19 1992-04-02 Robert Kerr Colonnes capillaires presentant une charge positive permanente utilisees en electrophorese capillaire
EP0541238A1 (en) * 1991-10-07 1993-05-12 Northeastern University Capillary column for high performance electrophoretic separation and detection of SDS proteins and system for using the same
WO1995020157A1 (en) * 1994-01-25 1995-07-27 Beckman Instruments, Inc. Coated capillary columns and electrophoretic separation methods for their use

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4680201A (en) * 1985-10-30 1987-07-14 Stellan Hjerten Coating for electrophoresis tube
EP0354984A2 (en) * 1988-08-19 1990-02-21 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow
EP0417925A2 (en) * 1989-09-12 1991-03-20 Northeastern University High performance microcapillary gel electrophoresis
WO1992005433A1 (fr) * 1990-09-19 1992-04-02 Robert Kerr Colonnes capillaires presentant une charge positive permanente utilisees en electrophorese capillaire
EP0541238A1 (en) * 1991-10-07 1993-05-12 Northeastern University Capillary column for high performance electrophoretic separation and detection of SDS proteins and system for using the same
WO1995020157A1 (en) * 1994-01-25 1995-07-27 Beckman Instruments, Inc. Coated capillary columns and electrophoretic separation methods for their use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2156476A1 (es) * 1998-02-16 2001-06-16 Consejo Superior Investigacion Tratamientos de colummnas capilares de silice fundida para la obtencion de capilares funcionalizados con grupos vinilo.

Also Published As

Publication number Publication date
ES2151354B1 (es) 2001-07-01

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