ES2143934B1 - AMPEROMETRIC AND VOLTAMPEROMETRIC QUANTIFICATION OF INDIGO BLUE FOR THE DETERMINATION OF HYDROLASES AND DEVELOPMENT OF BIOSENSORS. - Google Patents

AMPEROMETRIC AND VOLTAMPEROMETRIC QUANTIFICATION OF INDIGO BLUE FOR THE DETERMINATION OF HYDROLASES AND DEVELOPMENT OF BIOSENSORS.

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Publication number
ES2143934B1
ES2143934B1 ES9800126A ES9800126A ES2143934B1 ES 2143934 B1 ES2143934 B1 ES 2143934B1 ES 9800126 A ES9800126 A ES 9800126A ES 9800126 A ES9800126 A ES 9800126A ES 2143934 B1 ES2143934 B1 ES 2143934B1
Authority
ES
Spain
Prior art keywords
amperometric
hydrolases
quantification
development
biosensors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9800126A
Other languages
Spanish (es)
Other versions
ES2143934A1 (en
Inventor
Garcia Agustin Costa
Sanchez Cesar Fernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Oviedo
Original Assignee
Universidad de Oviedo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Oviedo filed Critical Universidad de Oviedo
Priority to ES9800126A priority Critical patent/ES2143934B1/en
Publication of ES2143934A1 publication Critical patent/ES2143934A1/en
Application granted granted Critical
Publication of ES2143934B1 publication Critical patent/ES2143934B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cuantificación amperométrica y voltamperométrica de azul de índigo para la determinación de hidrolasas y desarrollo de biosensores. Se utilizan derivados indólicos como sustratos enzimáticos que originan azul de índigo y fundamentan una detección amperométrica o voltamperométrica, tanto para el desarrollo puesta a punto de electroinmunoensayos enzimáticos inmunosensores con detección amperométrica y voltamperométrica, como para la cuantificación de hidrolasas.Amperometric and voltammetric quantification of indigo blue for the determination of hydrolases and development of biosensors. Indolic derivatives are used as enzyme substrates that originate indigo blue and are the basis for amperometric or voltammetric detection, both for the development of immunosensory enzyme electro-immunoassays with amperometric and voltammetric detection, and for the quantification of hydrolases.

ES9800126A 1998-01-23 1998-01-23 AMPEROMETRIC AND VOLTAMPEROMETRIC QUANTIFICATION OF INDIGO BLUE FOR THE DETERMINATION OF HYDROLASES AND DEVELOPMENT OF BIOSENSORS. Expired - Fee Related ES2143934B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9800126A ES2143934B1 (en) 1998-01-23 1998-01-23 AMPEROMETRIC AND VOLTAMPEROMETRIC QUANTIFICATION OF INDIGO BLUE FOR THE DETERMINATION OF HYDROLASES AND DEVELOPMENT OF BIOSENSORS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9800126A ES2143934B1 (en) 1998-01-23 1998-01-23 AMPEROMETRIC AND VOLTAMPEROMETRIC QUANTIFICATION OF INDIGO BLUE FOR THE DETERMINATION OF HYDROLASES AND DEVELOPMENT OF BIOSENSORS.

Publications (2)

Publication Number Publication Date
ES2143934A1 ES2143934A1 (en) 2000-05-16
ES2143934B1 true ES2143934B1 (en) 2000-12-16

Family

ID=8302496

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9800126A Expired - Fee Related ES2143934B1 (en) 1998-01-23 1998-01-23 AMPEROMETRIC AND VOLTAMPEROMETRIC QUANTIFICATION OF INDIGO BLUE FOR THE DETERMINATION OF HYDROLASES AND DEVELOPMENT OF BIOSENSORS.

Country Status (1)

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ES (1) ES2143934B1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4030995A (en) * 1976-07-13 1977-06-21 Sigma Chemical Company Alkaline phosphatase isoenzyme determination
US4748115A (en) * 1986-01-08 1988-05-31 Abbott Laboratories Substrate formulation in 2-amino-2-methyl-1-propanol buffer for alkaline phosphatase assays
CA2051144A1 (en) * 1990-09-12 1992-03-13 Akio Tsuji Enzyme assays

Also Published As

Publication number Publication date
ES2143934A1 (en) 2000-05-16

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