ES2139292T3 - METHOD AND CASE (KIT) TO PURIFY NUCLEIC ACIDS. - Google Patents

METHOD AND CASE (KIT) TO PURIFY NUCLEIC ACIDS.

Info

Publication number
ES2139292T3
ES2139292T3 ES96113114T ES96113114T ES2139292T3 ES 2139292 T3 ES2139292 T3 ES 2139292T3 ES 96113114 T ES96113114 T ES 96113114T ES 96113114 T ES96113114 T ES 96113114T ES 2139292 T3 ES2139292 T3 ES 2139292T3
Authority
ES
Spain
Prior art keywords
nucleic acids
kit
case
purify nucleic
purify
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96113114T
Other languages
Spanish (es)
Inventor
Melissa Reardon
Lisa Noeth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Corp
Original Assignee
Roche Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/434,324 external-priority patent/US4997932A/en
Application filed by Roche Diagnostics Corp filed Critical Roche Diagnostics Corp
Application granted granted Critical
Publication of ES2139292T3 publication Critical patent/ES2139292T3/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

UNA COMPOSICION PARA EL USO DE CELULAS DE LISINA QUE CONTIENEN ACIDOS NUCLEICOS QUE CONTIENEN TRIS, AEDT, UN SURFACTANTE ANFOTERICO, UN SURFACTANTE NEUTRAL Y GUANIDINA HCL.A COMPOSITION FOR THE USE OF LYSINE CELLS CONTAINING NUCLEIC ACIDS CONTAINING TRIS, AEDT, AN AMPHOTERIC SURFACTANT, A NEUTRAL SURFACTANT AND GUANIDINE HCL.

ES96113114T 1989-11-13 1990-07-31 METHOD AND CASE (KIT) TO PURIFY NUCLEIC ACIDS. Expired - Lifetime ES2139292T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/434,324 US4997932A (en) 1989-11-13 1989-11-13 Method and kit for purifying nucleic acids
EP90911986A EP0508985B1 (en) 1989-11-13 1990-07-31 Method and kit for purifying nucleic acids

Publications (1)

Publication Number Publication Date
ES2139292T3 true ES2139292T3 (en) 2000-02-01

Family

ID=26128493

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96113114T Expired - Lifetime ES2139292T3 (en) 1989-11-13 1990-07-31 METHOD AND CASE (KIT) TO PURIFY NUCLEIC ACIDS.

Country Status (1)

Country Link
ES (1) ES2139292T3 (en)

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