ES2137617T3 - FLUORIMETRY AND PHOSPHORIMETRY IN THE DOMAIN OF THE FREQUENCY OF CROSS CORRELATION OF HIGH SPEED. - Google Patents

FLUORIMETRY AND PHOSPHORIMETRY IN THE DOMAIN OF THE FREQUENCY OF CROSS CORRELATION OF HIGH SPEED.

Info

Publication number
ES2137617T3
ES2137617T3 ES96200207T ES96200207T ES2137617T3 ES 2137617 T3 ES2137617 T3 ES 2137617T3 ES 96200207 T ES96200207 T ES 96200207T ES 96200207 T ES96200207 T ES 96200207T ES 2137617 T3 ES2137617 T3 ES 2137617T3
Authority
ES
Spain
Prior art keywords
frequency
signal
cross correlation
pass
phosphorimetry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96200207T
Other languages
Spanish (es)
Inventor
Beniamino Barbieri
Enrico Gratton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
I S S USA Inc
Original Assignee
I S S USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/807,261 external-priority patent/US5212386A/en
Application filed by I S S USA Inc filed Critical I S S USA Inc
Application granted granted Critical
Publication of ES2137617T3 publication Critical patent/ES2137617T3/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Abstract

SE PRESENTA UN APARATO PARA LA FLUOROMETRIA Y/O FOSFORIMETRIA DE DOMINIO DE FRECUENCIA DE CORRELACION CRUZADA EN EL CUAL SE SUMINISTRAN MEDIOS PARA REALIZAR SECUENCIALMENTE RECORRIDOS DE FLUOROMETRIA Y/ FOSFORIMETRIA DE DOMINIO DE FRECUENCIA DE CORRELACION CRUZADA A UNA PRIMERA Y UNA SEGUNDA FRECUENCIAS SECUENCIALMENTE DIFERENTES. LAS INTENSIDADES DE LAS RESPUESTAS DE SEÑAL DE LAS PASADAS SON DETECTADAS EN LA FRECUENCIA DE CORRELACION DE CRUCE RESPECTIVA DE CADA PASADAS. LA DETECCION DE LA RESPUESTA DE SEÑAL SE PROLONGA EN CADA PASADA HASTA QUE UNA SEÑAL INTEGRADA CON UNA DESVIACION COMUN ESPECIFICADA HAYA SIDO ADQUIRIDA EN CADA UNA DE LAS PASADAS DIFERENTES. PREFERIBLEMENTE, LAS PASADAS SECUENCIALES SON EJECUTADAS AUTOMATICAMENTE POR UN PROGRAMA. TAMBIEN, LAS FORMAS DE LAS ONDAS MEDIDAS MEDIANTE LA DERIVACION DE LA RESPUESTA DE SEÑAL RESULTANTE EN CADA PASADA SON PLEGADAS. ESTO ES: LOS SEGMENTOS CORRESPONDIENTES DE LAS FORMAS DE LAS ONDAS SE SUPERPONEN PARA OBTENER UN VALOR DE FORMA DE ONDA MEDIO PARA CADA PASADA QUE TENGA UNA SEÑAL INCREMENTADA CON RESPECTO A LA RELACION DE RUIDO SOBRE LOS SEGMENTOS DE FORMA DE ONDAS INDIVIDUALES. TAMBIEN, PREFERIBLEMENTE, SE UTILIZA UN FILTRO DIGITAL DE FRECUENCIA VARIABLE CREADO MEDIANTE SOFTWARE PARA FILTRAR LAS RESPUESTAS DE SEÑAL EN LA FRECUENCIA DE CORRELACION DE CRUCE.AN APPARATUS IS PRESENTED FOR THE FLUOROMETRY AND / OR PHOSPHORIMETRY OF CRUZED CORRELATION FREQUENCY DOMAIN, WHICH IS SUPPLIED WITH MEANS TO SEQUENTIALLY SEARCH FLUOROMETRY, AND A FREQUENCY OF FREQUENCY, AND A FREQUENCY OF A FREQUENCY, THE STRENGTHS OF THE PASSING SIGNAL RESPONSES ARE DETECTED IN THE RESPECTIVE CROSS CORRELATION FREQUENCY OF EACH PASSING. THE DETECTION OF THE SIGNAL RESPONSE PROLONGS IN EACH PASS UNTIL AN INTEGRATED SIGNAL WITH A SPECIFIED COMMON DEVIATION HAS BEEN ACQUIRED IN EACH OF THE DIFFERENT PASSAGES. PREFERABLY, SEQUENTIAL PASSES ARE AUTOMATICALLY PERFORMED BY A PROGRAM. ALSO, THE SHAPES OF THE WAVES MEASURED BY DERIVING THE RESPONSE FROM THE RESULTING SIGNAL IN EACH PASS ARE FOLDED. THIS IS: THE CORRESPONDING SEGMENTS OF THE WAVE SHAPES ARE OVERLAPPED TO OBTAIN A MEAN WAVE SHAPE VALUE FOR EACH PASS THAT HAS AN INCREASED SIGNAL WITH RESPECT TO THE NOISE RATIO ON THE INDIVIDUAL WAVE SHAPE SEGMENTS. ALSO, PREFERABLY, A VARIABLE FREQUENCY DIGITAL FILTER CREATED THROUGH SOFTWARE IS USED TO FILTER THE SIGNAL RESPONSES IN THE CROSS CORRELATION FREQUENCY.

ES96200207T 1991-12-13 1992-12-09 FLUORIMETRY AND PHOSPHORIMETRY IN THE DOMAIN OF THE FREQUENCY OF CROSS CORRELATION OF HIGH SPEED. Expired - Lifetime ES2137617T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/807,261 US5212386A (en) 1991-12-13 1991-12-13 High speed cross-correlation frequency domain fluorometry-phosphorimetry
EP92311236A EP0551736B1 (en) 1991-12-13 1992-12-09 High speed cross-correlation frequency domain fluorometry-phosphorimetry

Publications (1)

Publication Number Publication Date
ES2137617T3 true ES2137617T3 (en) 1999-12-16

Family

ID=26132295

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96200207T Expired - Lifetime ES2137617T3 (en) 1991-12-13 1992-12-09 FLUORIMETRY AND PHOSPHORIMETRY IN THE DOMAIN OF THE FREQUENCY OF CROSS CORRELATION OF HIGH SPEED.

Country Status (1)

Country Link
ES (1) ES2137617T3 (en)

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