ES2136529A1 - sHSP gene promoters modified for specific expression in seeds and use in transgenic plants. - Google Patents

sHSP gene promoters modified for specific expression in seeds and use in transgenic plants.

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Publication number
ES2136529A1
ES2136529A1 ES009602746A ES9602746A ES2136529A1 ES 2136529 A1 ES2136529 A1 ES 2136529A1 ES 009602746 A ES009602746 A ES 009602746A ES 9602746 A ES9602746 A ES 9602746A ES 2136529 A1 ES2136529 A1 ES 2136529A1
Authority
ES
Spain
Prior art keywords
transgenic plants
seeds
modified
specific expression
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009602746A
Other languages
Spanish (es)
Other versions
ES2136529B1 (en
Inventor
Antolinez Concepcion Almoguera
Dapena Pilar Prieto
Fraga Juan Jordano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9602746A priority Critical patent/ES2136529B1/en
Publication of ES2136529A1 publication Critical patent/ES2136529A1/en
Application granted granted Critical
Publication of ES2136529B1 publication Critical patent/ES2136529B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

sHSP gene promoters modified for specific expression in seeds and use in transgenic plants. It is described how the promoter of the sunflower gene Ha Hsp17 7 G4 can be modified and chimeric genes obtained. These genes maintain their expression in the seed ripening phase and, owing to the modifications made in the regulatory sequences, they are not expressed during the drying of the embryo, nor do they respond to thermal shocks in transgenic plants. The modifications to the promoter improve the possible uses of said gene, or of others which contain said altered regulatory sequences. The promoters are applied in agriculture and have a potential use in obtaining seeds modified by genetic engineering techniques.
ES9602746A 1996-12-27 1996-12-27 PROMOTERS OF SHSP GENES MODIFIED FOR SPECIFIC EXPRESSION IN SEEDS AND USE IN TRANSGENIC PLANTS. Expired - Fee Related ES2136529B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9602746A ES2136529B1 (en) 1996-12-27 1996-12-27 PROMOTERS OF SHSP GENES MODIFIED FOR SPECIFIC EXPRESSION IN SEEDS AND USE IN TRANSGENIC PLANTS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9602746A ES2136529B1 (en) 1996-12-27 1996-12-27 PROMOTERS OF SHSP GENES MODIFIED FOR SPECIFIC EXPRESSION IN SEEDS AND USE IN TRANSGENIC PLANTS.

Publications (2)

Publication Number Publication Date
ES2136529A1 true ES2136529A1 (en) 1999-11-16
ES2136529B1 ES2136529B1 (en) 2000-04-01

Family

ID=8297158

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9602746A Expired - Fee Related ES2136529B1 (en) 1996-12-27 1996-12-27 PROMOTERS OF SHSP GENES MODIFIED FOR SPECIFIC EXPRESSION IN SEEDS AND USE IN TRANSGENIC PLANTS.

Country Status (1)

Country Link
ES (1) ES2136529B1 (en)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
COCA, M.A. et al. "Differential regulation of small heat-shock genes in plants: analysis of a water-stress-inducible and developmentally activated sunflower promoter" PLANT MOLECULAR BIOLOGY, 1996, Vol. 31, paginas 863-876 *
DULCE BARROS, M. et al. "Mutational analysis of a plant heat shock element" PLANT MOLECULAR BIOLOGY, 1992, Vol. 19, paginas 665-675 *
FERNANDES, M. et al. "Fine structure analyses of the Drosophila and Saccharomyces heat shock factor-heat shock element interactions" NUCLEIC ACIDS RESEARCH, 1994, Vol. 22, No 2, paginas 167-173 *

Also Published As

Publication number Publication date
ES2136529B1 (en) 2000-04-01

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