ES2133085A1 - Method of analysis of acute rejection in orthotropic liver transplant by determination of the intensity of expression of the co-stimulator molecules (CD28, CTLA-4, CD80 and CD86) on the surface of peripheral blood lymphocytes by flow cytometry - Google Patents

Method of analysis of acute rejection in orthotropic liver transplant by determination of the intensity of expression of the co-stimulator molecules (CD28, CTLA-4, CD80 and CD86) on the surface of peripheral blood lymphocytes by flow cytometry

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Publication number
ES2133085A1
ES2133085A1 ES09700050A ES9700050A ES2133085A1 ES 2133085 A1 ES2133085 A1 ES 2133085A1 ES 09700050 A ES09700050 A ES 09700050A ES 9700050 A ES9700050 A ES 9700050A ES 2133085 A1 ES2133085 A1 ES 2133085A1
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Spain
Prior art keywords
acute rejection
ctla
expression
intensity
flow cytometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09700050A
Other languages
Spanish (es)
Other versions
ES2133085B1 (en
Inventor
Lopez M Rocio Alvarez
Puras Alfredo Minguela
Alonso Ana Maria Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INSALUD MINISTERIO DE SANIDAD
HOSPITAL UNIVERSITARIO VIRGEN
Original Assignee
INSALUD MINISTERIO DE SANIDAD
HOSPITAL UNIVERSITARIO VIRGEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by INSALUD MINISTERIO DE SANIDAD, HOSPITAL UNIVERSITARIO VIRGEN filed Critical INSALUD MINISTERIO DE SANIDAD
Priority to ES9700050A priority Critical patent/ES2133085B1/en
Publication of ES2133085A1 publication Critical patent/ES2133085A1/en
Application granted granted Critical
Publication of ES2133085B1 publication Critical patent/ES2133085B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

Method of analysis of acute rejection in orthotropic liver transplant by determination of the intensity of expression of the co-stimulator molecules (CD28, CTLA-4, CD80 and CD86) on the surface of peripheral blood lymphocytes by flow cytometry. This discovery relates to a new process for early and bloodless detection of the onset of acute rejection in orthotropic liver transplant by investigating the intensity of expression of the co-stimulator molecules CD28, CTLA-4, CD80 and CD86 determined from their mean fluorescence channel estimated using flow cytometry techniques in peripheral blood cells extracted 3 days after transplant. Applications: this method can be used for the early detection and preventive treatment of episodes of acute rejection in orthotropic liver transplant, which offers the advantage of avoiding damage to the transplant and the use of more aggressive late treatments.
ES9700050A 1996-12-30 1996-12-30 METHOD OF ANALYSIS OF ACUTE REJECTION IN ORTHOTOPIC LIVER TRANSPLANTATION THROUGH THE DETERMINATION OF THE EXPRESSION INTENSITY OF THE CO-STIMULATING MOLECULES (CD28, CTLA-4, CD80 AND CD86) IN THE SURFACE OF LYMPHOCYTES OF PERIPHERAL BLOOD BY FLORA OF PERETHERAL BLOOD. Expired - Lifetime ES2133085B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9700050A ES2133085B1 (en) 1996-12-30 1996-12-30 METHOD OF ANALYSIS OF ACUTE REJECTION IN ORTHOTOPIC LIVER TRANSPLANTATION THROUGH THE DETERMINATION OF THE EXPRESSION INTENSITY OF THE CO-STIMULATING MOLECULES (CD28, CTLA-4, CD80 AND CD86) IN THE SURFACE OF LYMPHOCYTES OF PERIPHERAL BLOOD BY FLORA OF PERETHERAL BLOOD.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9700050A ES2133085B1 (en) 1996-12-30 1996-12-30 METHOD OF ANALYSIS OF ACUTE REJECTION IN ORTHOTOPIC LIVER TRANSPLANTATION THROUGH THE DETERMINATION OF THE EXPRESSION INTENSITY OF THE CO-STIMULATING MOLECULES (CD28, CTLA-4, CD80 AND CD86) IN THE SURFACE OF LYMPHOCYTES OF PERIPHERAL BLOOD BY FLORA OF PERETHERAL BLOOD.

Publications (2)

Publication Number Publication Date
ES2133085A1 true ES2133085A1 (en) 1999-08-16
ES2133085B1 ES2133085B1 (en) 2000-04-01

Family

ID=8297848

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9700050A Expired - Lifetime ES2133085B1 (en) 1996-12-30 1996-12-30 METHOD OF ANALYSIS OF ACUTE REJECTION IN ORTHOTOPIC LIVER TRANSPLANTATION THROUGH THE DETERMINATION OF THE EXPRESSION INTENSITY OF THE CO-STIMULATING MOLECULES (CD28, CTLA-4, CD80 AND CD86) IN THE SURFACE OF LYMPHOCYTES OF PERIPHERAL BLOOD BY FLORA OF PERETHERAL BLOOD.

Country Status (1)

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ES (1) ES2133085B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113981031A (en) * 2021-11-01 2022-01-28 山西中医药大学 Novel T cell function detection method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005912A1 (en) * 1986-03-31 1987-10-08 T Cell Sciences, Inc. Therapeutic and diagnostic methods using soluble t cell surface molecules
WO1994028912A1 (en) * 1993-06-10 1994-12-22 The Regents Of The University Of Michigan Cd28 pathway immunosuppression
WO1995034320A2 (en) * 1994-06-07 1995-12-21 Regents Of The University Of Minnesota Methods for inhibiting antigen specific t cell responses

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005912A1 (en) * 1986-03-31 1987-10-08 T Cell Sciences, Inc. Therapeutic and diagnostic methods using soluble t cell surface molecules
WO1994028912A1 (en) * 1993-06-10 1994-12-22 The Regents Of The University Of Michigan Cd28 pathway immunosuppression
WO1995034320A2 (en) * 1994-06-07 1995-12-21 Regents Of The University Of Minnesota Methods for inhibiting antigen specific t cell responses

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PAGE et al. "Human endothelial stimulation of allogeneic T cells via CTLA-4 independent pathway". 1994. TRANSPLANT IMMUNOLOGY, Vol. 2 (4), paginas 342-347. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113981031A (en) * 2021-11-01 2022-01-28 山西中医药大学 Novel T cell function detection method

Also Published As

Publication number Publication date
ES2133085B1 (en) 2000-04-01

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