ES2129001A1 - Metodo para la deteccion y visualizacion de una secuencia especifica de dna. - Google Patents

Metodo para la deteccion y visualizacion de una secuencia especifica de dna.

Info

Publication number
ES2129001A1
ES2129001A1 ES09701601A ES9701601A ES2129001A1 ES 2129001 A1 ES2129001 A1 ES 2129001A1 ES 09701601 A ES09701601 A ES 09701601A ES 9701601 A ES9701601 A ES 9701601A ES 2129001 A1 ES2129001 A1 ES 2129001A1
Authority
ES
Spain
Prior art keywords
displaying
detecting
dna sequence
specific dna
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09701601A
Other languages
English (en)
Other versions
ES2129001B1 (es
Inventor
Antonio Martinez
Juan Carlos Tercero
Emilio Valverde
Carmen Cabrero
Ana Diez
Amor Guerra
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmagen S A
Original Assignee
Pharmagen S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmagen S A filed Critical Pharmagen S A
Priority to ES009701601A priority Critical patent/ES2129001B1/es
Publication of ES2129001A1 publication Critical patent/ES2129001A1/es
Application granted granted Critical
Publication of ES2129001B1 publication Critical patent/ES2129001B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

El método comprende unir una sonda de DNA específica que se quiere detectar a un pocillo de una plaza microtiter, añadir el producto amplificado desnaturalizado, previamente marcado con dUTP y unido a moléculas de digoxigenina, añadir un anticuerpo antidigoxigenina marcado con una enzima que, en presencia de un sustrato adecuado da lugar a un producto de reacción coloreado, añadir un sustrato para dicha enzima que, tras la reacción, da, un producto coloreado, leer a la densidad óptica en la que el producto de la reacción presenta la máxima absorbancia, y evaluar el resultado comparando el valor de la absorbancia de un control negativo. En una realización particular, el método permite detectar la traslocación t(14; 18) que aparece en aproximadamente el 70% de los linfomas no-Hodgkin.
ES009701601A 1997-07-18 1997-07-18 Metodo para la deteccion y visualizacion de una secuencia especifica de dna. Expired - Fee Related ES2129001B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES009701601A ES2129001B1 (es) 1997-07-18 1997-07-18 Metodo para la deteccion y visualizacion de una secuencia especifica de dna.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009701601A ES2129001B1 (es) 1997-07-18 1997-07-18 Metodo para la deteccion y visualizacion de una secuencia especifica de dna.

Publications (2)

Publication Number Publication Date
ES2129001A1 true ES2129001A1 (es) 1999-05-16
ES2129001B1 ES2129001B1 (es) 2000-04-16

Family

ID=8300114

Family Applications (1)

Application Number Title Priority Date Filing Date
ES009701601A Expired - Fee Related ES2129001B1 (es) 1997-07-18 1997-07-18 Metodo para la deteccion y visualizacion de una secuencia especifica de dna.

Country Status (1)

Country Link
ES (1) ES2129001B1 (es)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006753A1 (en) * 1993-09-03 1995-03-09 The United States of America, represented by The Secretary, Department of Health & Human Services Method and compositions for primer specific solid-phase detection of pcr products
ES2083629T3 (es) * 1991-07-16 1996-04-16 Boehringer Mannheim Gmbh Inmovilizacion de acidos nucleicos.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2083629T3 (es) * 1991-07-16 1996-04-16 Boehringer Mannheim Gmbh Inmovilizacion de acidos nucleicos.
WO1995006753A1 (en) * 1993-09-03 1995-03-09 The United States of America, represented by The Secretary, Department of Health & Human Services Method and compositions for primer specific solid-phase detection of pcr products

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DE BEENHOWER, H. et al.: "Detection and identification of mycobacteria by DNA amplification and oligonucleotide-specific capture plate hybridization". 1995. J. Clin. Microbiol. Vol. 33 (11), paginas 2994-8. *
KING, J.A. et al.: "Detection of HIV-1 by digoxigenin-labeled PCR and microtiter plate solution hybridization assay and prevention of PCR carry-over by uracil-N-glycosylase". 1993. J. Virol. Methods. Vol. 44 (1), paginas 67-76. *
NEVINNY-STICKEL et al.: "HLA class II typing in a microtiter plate formate using digoxigenin-labeled amplified DNA and biotin-labeled oligonucleotide probes". 1993. Eur. J. Immunogenet. 1993. Vol. 20 (5), paginas 419-427. *

Also Published As

Publication number Publication date
ES2129001B1 (es) 2000-04-16

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