ES2127691A1 - Procedure for the covalent bonding of ligands for the preparation of immunochemical reagents for use in homogeneous immunoanalyses using microparticles (MPs) and its application to the measurement of ferritin and the soluble receptor for transferrin in blood serum or plasma - Google Patents

Procedure for the covalent bonding of ligands for the preparation of immunochemical reagents for use in homogeneous immunoanalyses using microparticles (MPs) and its application to the measurement of ferritin and the soluble receptor for transferrin in blood serum or plasma

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Publication number
ES2127691A1
ES2127691A1 ES09602216A ES9602216A ES2127691A1 ES 2127691 A1 ES2127691 A1 ES 2127691A1 ES 09602216 A ES09602216 A ES 09602216A ES 9602216 A ES9602216 A ES 9602216A ES 2127691 A1 ES2127691 A1 ES 2127691A1
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Spain
Prior art keywords
mps
procedure
ferritin
microparticles
ligands
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Application number
ES09602216A
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Spanish (es)
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ES2127691B1 (en
Inventor
Aguirre Rodriguez-Maimon
De Larrea Luis Angel Borque
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Biolatex S L
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Biolatex S L
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Application filed by Biolatex S L filed Critical Biolatex S L
Priority to ES9602216A priority Critical patent/ES2127691B1/en
Publication of ES2127691A1 publication Critical patent/ES2127691A1/en
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Publication of ES2127691B1 publication Critical patent/ES2127691B1/en
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Expired - Fee Related legal-status Critical Current

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Procedure for the covalent bonding of ligands for the preparation of immunochemical reagents for use in homogeneous immunoanalyses using microparticles (MPs) and its application to the measurement of ferritin and the soluble receptor for transferrin in blood serum or plasma. The procedure consists of a reagent for measuring substances in biological samples that includes the use of microparticles MPs in an aqueous suspension covered with an antibody Ac by means of a ligand-contraligand bridge. In this reagent the MPs have a contraligand such as avidin or streptavidin fixed on their surface and the specific antibodies are marked with a ligand such as biotin. In addition, the present invention describes a specific procedure for the covalent bonding of ligands on the Fc fraction of the antibodies that permits a spatial arrangement of them on the MPs that is very favourable to immunoaggregation. As a result of this spatial arrangement, the number of active antibodies is increased which therefore reduces the quantity of antibody needed to prepare the reagents. Also, and owing to this favourable spatial arrangement, non-specific reactions due to the rheumatoid factor are eliminated and the stability of the reagents is improved. The invention is applicable to the evaluation of the state of iron reserves by turbidimetric/nephalometric determination of the ferritin and the transfer receptors in blood serum or plasma.
ES9602216A 1996-10-21 1996-10-21 COVALENT JOINT PROCEDURE FOR LEAGUES FOR THE PREPARATION OF IMMUNOCHEMICAL R EAGENTS FOR USE IN HOMOGENEOUS IMMUNOANALYSIS USING MICROPARTICLES (MPS) AND THEIR APPLICATION TO THE MEASUREMENT OF FERRITINE AND SOLUBLE TRANSFERRAL RECEIVER IN SOUTH TRANSFERRINE Expired - Fee Related ES2127691B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9602216A ES2127691B1 (en) 1996-10-21 1996-10-21 COVALENT JOINT PROCEDURE FOR LEAGUES FOR THE PREPARATION OF IMMUNOCHEMICAL R EAGENTS FOR USE IN HOMOGENEOUS IMMUNOANALYSIS USING MICROPARTICLES (MPS) AND THEIR APPLICATION TO THE MEASUREMENT OF FERRITINE AND SOLUBLE TRANSFERRAL RECEIVER IN SOUTH TRANSFERRINE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9602216A ES2127691B1 (en) 1996-10-21 1996-10-21 COVALENT JOINT PROCEDURE FOR LEAGUES FOR THE PREPARATION OF IMMUNOCHEMICAL R EAGENTS FOR USE IN HOMOGENEOUS IMMUNOANALYSIS USING MICROPARTICLES (MPS) AND THEIR APPLICATION TO THE MEASUREMENT OF FERRITINE AND SOLUBLE TRANSFERRAL RECEIVER IN SOUTH TRANSFERRINE

Publications (2)

Publication Number Publication Date
ES2127691A1 true ES2127691A1 (en) 1999-04-16
ES2127691B1 ES2127691B1 (en) 2000-04-01

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ES9602216A Expired - Fee Related ES2127691B1 (en) 1996-10-21 1996-10-21 COVALENT JOINT PROCEDURE FOR LEAGUES FOR THE PREPARATION OF IMMUNOCHEMICAL R EAGENTS FOR USE IN HOMOGENEOUS IMMUNOANALYSIS USING MICROPARTICLES (MPS) AND THEIR APPLICATION TO THE MEASUREMENT OF FERRITINE AND SOLUBLE TRANSFERRAL RECEIVER IN SOUTH TRANSFERRINE

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109164263A (en) * 2018-09-05 2019-01-08 苏州普瑞斯生物科技有限公司 A kind of kit and preparation method measuring light chain Kappa concentration

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105181962B (en) * 2015-09-02 2016-09-14 郁东 A kind of rheumatoid factor detection reagent

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991010747A1 (en) * 1990-01-16 1991-07-25 Prutech Research Development Partnership Ii Combination assay for antibody or antigen
US5212063A (en) * 1989-05-09 1993-05-18 Boehringer Mannheim Gmbh Ligand trap useful in immunoassays of biotin or free biotin containing samples and improved immunoassays using these ligand traps

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212063A (en) * 1989-05-09 1993-05-18 Boehringer Mannheim Gmbh Ligand trap useful in immunoassays of biotin or free biotin containing samples and improved immunoassays using these ligand traps
WO1991010747A1 (en) * 1990-01-16 1991-07-25 Prutech Research Development Partnership Ii Combination assay for antibody or antigen

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LUIS BORQUE LARREA et al. The determination of Rheumatoid Factors by Immunoturbidimetric Assay on Boehringer Mannheim/Hitachi Analysis Systems. Kim. Lab. 1994; 40; paginas 445-453 *
M. LIEVENS et al. Determination of transferrin without sample predilution by a turbidimetric assay on Boehringer Mannheim/Hitachi analysis systems. Lab. med. 18/3 (1994) paginas 83-90. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109164263A (en) * 2018-09-05 2019-01-08 苏州普瑞斯生物科技有限公司 A kind of kit and preparation method measuring light chain Kappa concentration

Also Published As

Publication number Publication date
ES2127691B1 (en) 2000-04-01

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