ES2127691A1 - Procedure for the covalent bonding of ligands for the preparation of immunochemical reagents for use in homogeneous immunoanalyses using microparticles (MPs) and its application to the measurement of ferritin and the soluble receptor for transferrin in blood serum or plasma - Google Patents
Procedure for the covalent bonding of ligands for the preparation of immunochemical reagents for use in homogeneous immunoanalyses using microparticles (MPs) and its application to the measurement of ferritin and the soluble receptor for transferrin in blood serum or plasmaInfo
- Publication number
- ES2127691A1 ES2127691A1 ES09602216A ES9602216A ES2127691A1 ES 2127691 A1 ES2127691 A1 ES 2127691A1 ES 09602216 A ES09602216 A ES 09602216A ES 9602216 A ES9602216 A ES 9602216A ES 2127691 A1 ES2127691 A1 ES 2127691A1
- Authority
- ES
- Spain
- Prior art keywords
- mps
- procedure
- ferritin
- microparticles
- ligands
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Procedure for the covalent bonding of ligands for the preparation of immunochemical reagents for use in homogeneous immunoanalyses using microparticles (MPs) and its application to the measurement of ferritin and the soluble receptor for transferrin in blood serum or plasma. The procedure consists of a reagent for measuring substances in biological samples that includes the use of microparticles MPs in an aqueous suspension covered with an antibody Ac by means of a ligand-contraligand bridge. In this reagent the MPs have a contraligand such as avidin or streptavidin fixed on their surface and the specific antibodies are marked with a ligand such as biotin. In addition, the present invention describes a specific procedure for the covalent bonding of ligands on the Fc fraction of the antibodies that permits a spatial arrangement of them on the MPs that is very favourable to immunoaggregation. As a result of this spatial arrangement, the number of active antibodies is increased which therefore reduces the quantity of antibody needed to prepare the reagents. Also, and owing to this favourable spatial arrangement, non-specific reactions due to the rheumatoid factor are eliminated and the stability of the reagents is improved. The invention is applicable to the evaluation of the state of iron reserves by turbidimetric/nephalometric determination of the ferritin and the transfer receptors in blood serum or plasma.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9602216A ES2127691B1 (en) | 1996-10-21 | 1996-10-21 | COVALENT JOINT PROCEDURE FOR LEAGUES FOR THE PREPARATION OF IMMUNOCHEMICAL R EAGENTS FOR USE IN HOMOGENEOUS IMMUNOANALYSIS USING MICROPARTICLES (MPS) AND THEIR APPLICATION TO THE MEASUREMENT OF FERRITINE AND SOLUBLE TRANSFERRAL RECEIVER IN SOUTH TRANSFERRINE |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9602216A ES2127691B1 (en) | 1996-10-21 | 1996-10-21 | COVALENT JOINT PROCEDURE FOR LEAGUES FOR THE PREPARATION OF IMMUNOCHEMICAL R EAGENTS FOR USE IN HOMOGENEOUS IMMUNOANALYSIS USING MICROPARTICLES (MPS) AND THEIR APPLICATION TO THE MEASUREMENT OF FERRITINE AND SOLUBLE TRANSFERRAL RECEIVER IN SOUTH TRANSFERRINE |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2127691A1 true ES2127691A1 (en) | 1999-04-16 |
ES2127691B1 ES2127691B1 (en) | 2000-04-01 |
Family
ID=8296425
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9602216A Expired - Fee Related ES2127691B1 (en) | 1996-10-21 | 1996-10-21 | COVALENT JOINT PROCEDURE FOR LEAGUES FOR THE PREPARATION OF IMMUNOCHEMICAL R EAGENTS FOR USE IN HOMOGENEOUS IMMUNOANALYSIS USING MICROPARTICLES (MPS) AND THEIR APPLICATION TO THE MEASUREMENT OF FERRITINE AND SOLUBLE TRANSFERRAL RECEIVER IN SOUTH TRANSFERRINE |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2127691B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109164263A (en) * | 2018-09-05 | 2019-01-08 | 苏州普瑞斯生物科技有限公司 | A kind of kit and preparation method measuring light chain Kappa concentration |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105181962B (en) * | 2015-09-02 | 2016-09-14 | 郁东 | A kind of rheumatoid factor detection reagent |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991010747A1 (en) * | 1990-01-16 | 1991-07-25 | Prutech Research Development Partnership Ii | Combination assay for antibody or antigen |
US5212063A (en) * | 1989-05-09 | 1993-05-18 | Boehringer Mannheim Gmbh | Ligand trap useful in immunoassays of biotin or free biotin containing samples and improved immunoassays using these ligand traps |
-
1996
- 1996-10-21 ES ES9602216A patent/ES2127691B1/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5212063A (en) * | 1989-05-09 | 1993-05-18 | Boehringer Mannheim Gmbh | Ligand trap useful in immunoassays of biotin or free biotin containing samples and improved immunoassays using these ligand traps |
WO1991010747A1 (en) * | 1990-01-16 | 1991-07-25 | Prutech Research Development Partnership Ii | Combination assay for antibody or antigen |
Non-Patent Citations (2)
Title |
---|
LUIS BORQUE LARREA et al. The determination of Rheumatoid Factors by Immunoturbidimetric Assay on Boehringer Mannheim/Hitachi Analysis Systems. Kim. Lab. 1994; 40; paginas 445-453 * |
M. LIEVENS et al. Determination of transferrin without sample predilution by a turbidimetric assay on Boehringer Mannheim/Hitachi analysis systems. Lab. med. 18/3 (1994) paginas 83-90. * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109164263A (en) * | 2018-09-05 | 2019-01-08 | 苏州普瑞斯生物科技有限公司 | A kind of kit and preparation method measuring light chain Kappa concentration |
Also Published As
Publication number | Publication date |
---|---|
ES2127691B1 (en) | 2000-04-01 |
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Legal Events
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EC2A | Search report published |
Date of ref document: 19990416 Kind code of ref document: A1 Effective date: 19990416 |
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Effective date: 20180807 |