ES2121561B1 - NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE. - Google Patents

NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE.

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Publication number
ES2121561B1
ES2121561B1 ES9701003A ES9701003A ES2121561B1 ES 2121561 B1 ES2121561 B1 ES 2121561B1 ES 9701003 A ES9701003 A ES 9701003A ES 9701003 A ES9701003 A ES 9701003A ES 2121561 B1 ES2121561 B1 ES 2121561B1
Authority
ES
Spain
Prior art keywords
detection
thermostable
nitrates
applicable
high temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9701003A
Other languages
Spanish (es)
Other versions
ES2121561A1 (en
Inventor
Carlos Jose Berenguer
Arcos Sandra Ramirez
Grado Sanz Myriam De
Herrero Luis Angel Fernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Autonoma de Madrid
Original Assignee
Universidad Autonoma de Madrid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Autonoma de Madrid filed Critical Universidad Autonoma de Madrid
Priority to ES9701003A priority Critical patent/ES2121561B1/en
Publication of ES2121561A1 publication Critical patent/ES2121561A1/en
Application granted granted Critical
Publication of ES2121561B1 publication Critical patent/ES2121561B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

NITRATO REDUCTASA TERMOESTABLE APLICABLE PARA LA DETECCION DE NITRATOS DE ALTAS TEMPERATURAS, QUE CONSISTE EN CLONAR LA REGION DE ADN QUE CODIFICA LA NITRATO REDUCTASA TERMOESTABLE APLICABLE PARA LA DETECCION DE NITRATOS A ALTA TEMPERATURA, A PARTIR DE UNA GENOTECA DE ADN DE LA BACTERIA THERMUS THERMOPHILUS HB8, UTILIZANDO PARA ELLO UNA SONDA DE ADN ESPECIFICA PARA SU DETECCION. UN FRAGMENTO DE 8.2 KILOPARES DE BASES, COMPRENDIDO ENTRE DOS DIANAS DE RESTRICCION PSTI FUE AISLADO A PARTIR DE ESTA REGION Y CLONADO EN EL VECTOR BIFUNCIONAL PMK18. EL VECTOR PMK18 ESTA CARACTERIZADO POR POSEER DOS ORIGENES DE REPLICACION FUNCIONALES DE ESCHERICHIA COLI Y THERMUS THERMOPHILUS, UN GEN QUE CONFIERE RESISTENCIA TERMOESTABLE A KANAMICINA PROTETIDO POR LA PATENTE DE INVENCION 9301522 SOLICITADA EN ESPAÑA, UN PROMOTOR INDUCIBLE EN ESCHERICHIA COLI Y UNA REGION DE CLONAJE CON DOS DIANAS DE RESTRICCION MULTIPLES. CUANDO ESTA CONSTRUCCION SE INTRODUCE EN THERMUS THERMOPHILUS HB27 O EN ESCHERICHIA COLI, SESINTETIZA UNA ENZIMA SOLUBLE DE 192 KILODALTONES DE TALLA MOLECULAR, CAPAZ DE CATALIZAR LA REDUCCION DE NITRATO O NITRITO A UNA TEMPERATURA OPTIMA DE 80 C.NITRATE REDUCTASA APPLICABLE FOR THE DETECTION OF NITRATES OF HIGH TEMPERATURES, WHICH CONSISTS IN CLONING THE REGION OF DNA THAT CODES THE THERMOSTABLE NITRATE APPLICABLE FOR THE DETECTION OF NITRATES OF HIGH TEMPERATURE, A PARTICIPATED BY THE GENUINE DEPARTMENT OF A HIGH TEMPERATURE USING A SPECIFIC DNA PROBE FOR IT DETECTION. A FRAGMENT OF 8.2 KILOPARS OF BASES, INCLUDED BETWEEN TWO DAYS OF RESTRICTION PSTI WAS ISOLATED FROM THIS REGION AND CLONED IN THE BIFUNCTIONAL VECTOR PMK18. THE VECTOR PMK18 IS CHARACTERIZED BY POSSESSING TWO FUNCTIONAL REPLICATION SOURCES OF ESCHERICHIA COLI AND THERMUS THERMOPHILUS, A GENE THAT CONFERTS THERMOSTABLE RESISTANCE TO KANAMYCIN PROVIDED BY A REGIOUS PROJECT AND A PROMOTE ENCIIERA ENCYRONIA, A PROMOTOR TWO DAYS OF MULTIPLE RESTRICTION. WHEN THIS CONSTRUCTION INTRODUCES ITSELF IN THERMUS THERMOPHILUS HB27 OR IN ESCHERICHIA COLI, IT MAKES A SOLUBLE ENZYME OF 192 KILODALLONS OF MOLECULAR SIZE, CAPABLE OF CATALYZING THE REDUCTION OF NITRATE OR NITRITE AT AN OPTIMAL TEMPERATURE OF 80 C.

ES9701003A 1997-05-09 1997-05-09 NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE. Expired - Fee Related ES2121561B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9701003A ES2121561B1 (en) 1997-05-09 1997-05-09 NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9701003A ES2121561B1 (en) 1997-05-09 1997-05-09 NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE.

Publications (2)

Publication Number Publication Date
ES2121561A1 ES2121561A1 (en) 1998-11-16
ES2121561B1 true ES2121561B1 (en) 1999-05-16

Family

ID=8299246

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9701003A Expired - Fee Related ES2121561B1 (en) 1997-05-09 1997-05-09 NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE.

Country Status (1)

Country Link
ES (1) ES2121561B1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8900982L (en) * 1989-03-20 1990-09-21 Iceland Tech Inst INSULATION OF A NEW THERMOPHIL BACTERIAL THERMUS ANAEROBICUS PREPARATION AND PREPARATION OF A NEW FORMAT-BONDED NITRATE REDUCTION SYSTEM OF THIS FOR ANALYTICAL AND COMMERCIAL USE.
ES2067414B1 (en) * 1993-07-07 1995-09-16 Univ Madrid CHEMICAL GENE ABLE TO CONFER THERMOSTABLE KANAMYCIN RESISTANCE IN THERMUS THERMUS BACTERIA.

Also Published As

Publication number Publication date
ES2121561A1 (en) 1998-11-16

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