ES2121561B1 - NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE. - Google Patents
NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE.Info
- Publication number
- ES2121561B1 ES2121561B1 ES9701003A ES9701003A ES2121561B1 ES 2121561 B1 ES2121561 B1 ES 2121561B1 ES 9701003 A ES9701003 A ES 9701003A ES 9701003 A ES9701003 A ES 9701003A ES 2121561 B1 ES2121561 B1 ES 2121561B1
- Authority
- ES
- Spain
- Prior art keywords
- detection
- thermostable
- nitrates
- applicable
- high temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
NITRATO REDUCTASA TERMOESTABLE APLICABLE PARA LA DETECCION DE NITRATOS DE ALTAS TEMPERATURAS, QUE CONSISTE EN CLONAR LA REGION DE ADN QUE CODIFICA LA NITRATO REDUCTASA TERMOESTABLE APLICABLE PARA LA DETECCION DE NITRATOS A ALTA TEMPERATURA, A PARTIR DE UNA GENOTECA DE ADN DE LA BACTERIA THERMUS THERMOPHILUS HB8, UTILIZANDO PARA ELLO UNA SONDA DE ADN ESPECIFICA PARA SU DETECCION. UN FRAGMENTO DE 8.2 KILOPARES DE BASES, COMPRENDIDO ENTRE DOS DIANAS DE RESTRICCION PSTI FUE AISLADO A PARTIR DE ESTA REGION Y CLONADO EN EL VECTOR BIFUNCIONAL PMK18. EL VECTOR PMK18 ESTA CARACTERIZADO POR POSEER DOS ORIGENES DE REPLICACION FUNCIONALES DE ESCHERICHIA COLI Y THERMUS THERMOPHILUS, UN GEN QUE CONFIERE RESISTENCIA TERMOESTABLE A KANAMICINA PROTETIDO POR LA PATENTE DE INVENCION 9301522 SOLICITADA EN ESPAÑA, UN PROMOTOR INDUCIBLE EN ESCHERICHIA COLI Y UNA REGION DE CLONAJE CON DOS DIANAS DE RESTRICCION MULTIPLES. CUANDO ESTA CONSTRUCCION SE INTRODUCE EN THERMUS THERMOPHILUS HB27 O EN ESCHERICHIA COLI, SESINTETIZA UNA ENZIMA SOLUBLE DE 192 KILODALTONES DE TALLA MOLECULAR, CAPAZ DE CATALIZAR LA REDUCCION DE NITRATO O NITRITO A UNA TEMPERATURA OPTIMA DE 80 C.NITRATE REDUCTASA APPLICABLE FOR THE DETECTION OF NITRATES OF HIGH TEMPERATURES, WHICH CONSISTS IN CLONING THE REGION OF DNA THAT CODES THE THERMOSTABLE NITRATE APPLICABLE FOR THE DETECTION OF NITRATES OF HIGH TEMPERATURE, A PARTICIPATED BY THE GENUINE DEPARTMENT OF A HIGH TEMPERATURE USING A SPECIFIC DNA PROBE FOR IT DETECTION. A FRAGMENT OF 8.2 KILOPARS OF BASES, INCLUDED BETWEEN TWO DAYS OF RESTRICTION PSTI WAS ISOLATED FROM THIS REGION AND CLONED IN THE BIFUNCTIONAL VECTOR PMK18. THE VECTOR PMK18 IS CHARACTERIZED BY POSSESSING TWO FUNCTIONAL REPLICATION SOURCES OF ESCHERICHIA COLI AND THERMUS THERMOPHILUS, A GENE THAT CONFERTS THERMOSTABLE RESISTANCE TO KANAMYCIN PROVIDED BY A REGIOUS PROJECT AND A PROMOTE ENCIIERA ENCYRONIA, A PROMOTOR TWO DAYS OF MULTIPLE RESTRICTION. WHEN THIS CONSTRUCTION INTRODUCES ITSELF IN THERMUS THERMOPHILUS HB27 OR IN ESCHERICHIA COLI, IT MAKES A SOLUBLE ENZYME OF 192 KILODALLONS OF MOLECULAR SIZE, CAPABLE OF CATALYZING THE REDUCTION OF NITRATE OR NITRITE AT AN OPTIMAL TEMPERATURE OF 80 C.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701003A ES2121561B1 (en) | 1997-05-09 | 1997-05-09 | NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9701003A ES2121561B1 (en) | 1997-05-09 | 1997-05-09 | NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2121561A1 ES2121561A1 (en) | 1998-11-16 |
ES2121561B1 true ES2121561B1 (en) | 1999-05-16 |
Family
ID=8299246
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9701003A Expired - Fee Related ES2121561B1 (en) | 1997-05-09 | 1997-05-09 | NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2121561B1 (en) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE8900982L (en) * | 1989-03-20 | 1990-09-21 | Iceland Tech Inst | INSULATION OF A NEW THERMOPHIL BACTERIAL THERMUS ANAEROBICUS PREPARATION AND PREPARATION OF A NEW FORMAT-BONDED NITRATE REDUCTION SYSTEM OF THIS FOR ANALYTICAL AND COMMERCIAL USE. |
ES2067414B1 (en) * | 1993-07-07 | 1995-09-16 | Univ Madrid | CHEMICAL GENE ABLE TO CONFER THERMOSTABLE KANAMYCIN RESISTANCE IN THERMUS THERMUS BACTERIA. |
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1997
- 1997-05-09 ES ES9701003A patent/ES2121561B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2121561A1 (en) | 1998-11-16 |
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Legal Events
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Date of ref document: 19981116 Kind code of ref document: A1 Effective date: 19981116 |
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Effective date: 20170825 |